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Annals of Forest Science

, 75:75 | Cite as

Genetic structure and phylogeography of Juniperus phoenicea complex throughout Mediterranean and Macaronesian regions: different stories in one

  • Pedro Sánchez-Gómez
  • Juan F. Jiménez
  • Jose Luis Cánovas
  • Juan Bautista Vera
  • Isabell Hensen
  • Miloud Aouissat
Research Paper

Abstract

Key message

The genetic structure of Juniperus phoenicea in the Mediterranean Basin is inferred using amplified fragment length polymorphism markers (AFLP) markers. As other Mediterranean conifers, J. phoenicea populations show moderate levels of genetic diversity and interpopulational differentiation. The pattern of distribution of genetic diversity seems highly influenced by the climatic fluctuations which occurred in the Pleistocene.

Context

It has been stated that the genetic structure of Mediterranean conifers is mediated by the historical climatic changes and the geological rearrangements which occurred in the Mediterranean Basin. J. phoenicea provides an excellent example to test how its genetic structure is influenced by these events.

Aims

In this work, we study the amount and distribution of genetic diversity of J. phoenicea complex, in order to evaluate its taxonomic status and to reveal underlying phylogeographic patterns.

Methods

The molecular diversity was analyzed for 805 individuals from 46 populations throughout its distribution range using AFLP markers. Principal coordinate analysis, analysis of molecular variance (AMOVA), and Bayesian-based analysis were applied to examine the population structure.

Results

AFLP markers revealed moderate levels of intrapopulation genetic diversity, pairwise genetic differentiation, and a clear pattern of isolation by distance. Bayesian analysis of population structure showed five clusters related to the taxonomic status of J. phoenicea and J. turbinata, and a geographic pattern of genetic structure in J. turbinata.

Conclusion

All the analysis separate J. phoenicea from J. turbinata. For J. turbinata, up to four groups can be distinguished from a phylogeographic point of view. The genetic structure of J. turbinata seems highly influenced by climatic and geologic fluctuations occurring since the Oligocene.

Keywords

AFLP markers Biogeography Cupressaceae Genetic diversity Inter-populational differentiation Taxonomy 

Notes

Acknowledgements

Authors wish to thank to Jaime Güemes, Gianluigi Bachetta, Jorge Balibrea, Laura Aznar, Jesús Muñoz, Mohammad Al-Gharaibeh, and David López for field assistance in collecting samples.

Funding

This work has been supported by a Ministry of Economy and Competitiveness CGL2011-30099 project grant to Pedro Sánchez Gómez.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13595_2018_741_MOESM1_ESM.xls (58 kb)
Table S1 Pairwise genetic differentiation among populations. Upper section, Fst values obtained with AMOVA. Lower section, Fst values obtained with AFLPsurv. (XLS 57 kb)
13595_2018_741_Fig5_ESM.png (1.3 mb)
Fig. S1

Map of sampled populations. Codes of populations are listed in Table 1 (PNG 1.28 mb)

13595_2018_741_MOESM2_ESM.tif (690 kb)
High resolution image (TIF 690 kb)
13595_2018_741_Fig6_ESM.png (1.4 mb)
Fig. S2

Neighbour-joining phenogram based on Nei's unbiased genetic distance for the 46 populations. Bootstrap values (>70%) over loci (based on 1000 replicates) are indicated for each node (PNG 1.39 mb)

13595_2018_741_MOESM3_ESM.tif (536 kb)
High resolution image (TIF 536 kb)

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Copyright information

© INRA and Springer-Verlag France SAS, part of Springer Nature 2018

Authors and Affiliations

  • Pedro Sánchez-Gómez
    • 1
  • Juan F. Jiménez
    • 1
    • 2
  • Jose Luis Cánovas
    • 1
  • Juan Bautista Vera
    • 1
  • Isabell Hensen
    • 3
  • Miloud Aouissat
    • 4
  1. 1.Departamento de Biología Vegetal (Botánica)Universidad de MurciaMurciaSpain
  2. 2.Departamento de Botánica, Facultad de CienciasUniversidad de GranadaGranadaSpain
  3. 3.Institut für BiologieMartin-Luther UniversitatHalleGermany
  4. 4.Centre Universitaire Salhi Ahmed NaamaNaâmaAlgeria

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