Thinning affects microbial biomass without changing enzyme activity in the soil of Pinus densiflora Sieb. et Zucc. forests after 7 years
- 110 Downloads
Thinning increased microbial biomass but did not alter enzyme activities in the soil of Pinus densiflora Sieb. et Zucc. forests in South Korea. This effect of thinning was larger under a relatively heavy thinning intensity, but there was divergence in the magnitude between sites.
The balance between microbial biomass accumulation and enzymatic C and N assimilation determines the level of bio-available C and N. However, the effects of thinning on these parameters remain contradictory and unconfirmed.
The effects of thinning intensity on microbial biomass and enzyme activity were assessed in the soil of Pinus densiflora Sieb. et Zucc. forests in South Korea.
Un-thinned control and 15 and 30% basal area thinning treatments were applied to two 51- to 60-year-old P. densiflora forests with different management histories, topographies, rainfall amounts, and soils. Seven years after thinning, microbial biomass and activities of N-acetyl-glucosaminidase, β-glucosidase, cellobiohydrolase, β-xylosidase, phenol oxidase, and peroxidase were measured before and after seasonally concentrated rains and at 0–10 cm depth.
Microbial biomass was generally highest under the 30% basal area thinning and lowest under the control, and was positively correlated to total soil C and N. The increase in microbial biomass was lower at the site displaying sandier, drier, and more acidic soils and retaining smaller amounts of thinning residue. Conversely, thinning had no significant effect on activities of all enzymes at both sites in both periods.
Thinning can promote accumulation of microbial biomass without significant change in enzyme activities participating in the assimilation of C and N. This effect of thinning tended to increase with thinning intensity but differed in magnitude between sites.
KeywordsEnzyme assay Forest management Korean red pine Soil microbes
We thank Jongyeol LEE, Sohye LEE, Hanna CHANG, Hyeon Min YUN, Min Ji PARK, Suwon CHOI, Jiae AN, and Yujin ROH for their assistance in both field and laboratory.
The present study was supported by the National Institute of Forest Science (project number FM0101-2009-01) and the Korea University (2017).
- Baena CW, Andrés-Abellán M, Lucas-Borja ME, Martínez-García E, García-Morote FA, Rubio E, López-Serrano FR (2013) Thinning and recovery effects on soil properties in two sites of a Mediterranean forest, in Cuenca Mountain (south-eastern of Spain). For Ecol Manag 308:223–230. https://doi.org/10.1016/j.foreco.2013.06.065 CrossRefGoogle Scholar
- Benesch M, Glaser B, Dippold M, Zech W (2015) Soil microbial C and N turnover under Cupressus lusitanica and natural forests in southern Ethiopia assessed by decomposition of 13C- and 15N-labelled litter under field conditions. Plant Soil 388:133–146. https://doi.org/10.1007/s11104-014-2317-0 CrossRefGoogle Scholar
- Flint LE, Flint AL (2002) Porosity. In: Campbell GS, Horton R, Jury WA, Nielsen DR, van Es HM, Wierenga PJ, Dane JH, Topp GC (eds) Methods of soil analysis. Part 4-physical methods. SSSA, Wisconsin, pp 255–293Google Scholar
- Gee GW, Or D (2002) Particle-size analysis. In: Campbell GS, Horton R, Jury WA, Nielsen DR, van Es HM, Wierenga PJ, Dane JH, Topp GC (eds) Methods of soil analysis. Part 4-physical methods. SSSA, Wisconsin, pp 255–293Google Scholar
- Korea Forest Research Institute (2012) Forestry handbook. Korea Forest Research Institute, Seoul (in Korean) Google Scholar
- Korea Forest Service (2015) Statistical yearbook of forestry. Korea Forest Service, Daejeon (in Korean) Google Scholar
- Korea Meteorological Administration (2011) Climatological normals of Korea. Korea Meteorological Administration, Seoul (in Korean) Google Scholar
- Korea Meteorological Administration (2015) Annual climatological report. Korea Meteorological Administration, Seoul (in Korean) Google Scholar
- Lee CS, Kim JH (1987) Relationships between soil factors and growth of annual ring in Pinus densiflora on stony mountain. Korea J Ecol 10:151–159Google Scholar
- Mulvaney RL (1996) Nitrogen-inorganic forms. In: Sparks DL, Page AL, Helmke PA, Loeppert RH, Soltanpour PN, Tabatabai MA, Johnston CT, Sumner ME (eds) Methods of soil analysis. Part 3-chemical methods. SSSA and ASA, Wisconsin, pp 1146–1155Google Scholar
- Nelson DW, Sommers LE (1996) Total carbon, organic carbon, and organic matter. In: Sparks DL, Page AL, Helmke PA, Loeppert RH, Soltanpour PN, Tabatabai MA, Johnston CT, Sumner ME (eds) Methods of soil analysis. Part 3-chemical methods. SSSA and ASA, Wisconsin, pp 961–1010Google Scholar
- Page-Dumroese DS, Jurgensen MF, Brown RE, Mroz GD (1999) Comparison of methods for determining bulk densities of rocky forest soils. Soil Sci Soc Am J 63:379–383. https://doi.org/10.2136/sssaj1999.03615995006300020016x CrossRefGoogle Scholar
- Park J, Matzner E (2003) Controls on the release of dissolved organic carbon and nitrogen from a deciduous forest floor investigated by manipulations of aboveground litter inputs and water flux. Biogeochemistry 66:265–286. https://doi.org/10.1023/B:BIOG.0000005341.19412.7b CrossRefGoogle Scholar
- Perakis SS, Hedin LO (2001) Fluxes and fates of nitrogen in soil of an unpolluted old-growth temperate forest, southern Chile. Ecology 82(8):2245–2260. https://doi.org/10.1890/0012-9658(2001)082[2245:FAFONI]2.0.CO;2Google Scholar
- Tan X, Chang SX, Comeau PG, Wang Y (2008) Thinning effects on microbial biomass, N mineralization, and tree growth in a mid-rotation fire-origin lodgepole pine stand in the lower foothills of Alberta, Canada. For Sci 54:465–474Google Scholar