Annals of Forest Science

, Volume 69, Issue 1, pp 49–57 | Cite as

ITS-based comparison of endophytic mycota in twigs of native Populus nigra and cultivated P. x euramericana (cv. I-214) stands in Northern Spain

  • Jorge Martín-García
  • Michael M. Müller
  • Julio Javier Diez
Original Paper
First Online:
Received:
Accepted:

Abstract

Context

The European black poplar (Populus nigra) is considered one of the most seriously endangered indigenous tree species. However, the total area covered by monoclonal plantations of hybrid poplar is growing, because of the high profitability of these trees.

Aim

The aim of this study is to describe and compare the endophytic mycota in twigs from native poplar (P. nigra) and hybrid poplar plantations (P. x euramericana clone I-214).

Methods

Twig endophytes were isolated from three native and three hybrid poplar stands in Palencia (N. Spain). Three trees were chosen at random within each stand sampled. Isolates were identified according to sequences of the internal transcribed spacer region of their rDNA. The study focused on dominant species.

Results

A total of 13 species were found. Six species originated from P. nigra only (Stemphylium vesicarium, Alternaria spp., Aureobasidium pullulans, Fimetariella rabenhorstii, Plagiostoma fraxini and Cryptodiaporthe salicella) and four from P. x euramericana only (Cytospora chrysosperma, Athelia bombacina, Biscogniauxia mediterranea and Unidentified sp. 1), whereas three were found on both types of trees (Lewia infectoria, Alternaria alternata and Epicoccum nigrum).

Conclusion

The results show that the endophyte community on poplar twigs differs between native and hybrid poplars

Keywords

Native Hybrid Poplar Endophyte Twig 
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Notes

Acknowledgements

We thank Marja-Leena Santanen and Antonio Sanz for their technical assistance. The authors also thank Leticia Botella for helpful comments on earlier versions of the manuscript. Christine Francis checked the English version and provided generous linguistic advice.

Funding

Financial support for this study was provided by the European Union and the Regional Government of Castile and Leon, through the INTERREG IIIB Atlantic Area programme (FORSEE project).

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Copyright information

© INRA and Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Jorge Martín-García
    • 1
    • 2
  • Michael M. Müller
    • 3
  • Julio Javier Diez
    • 1
  1. 1.Sustainable Forest Management Research InstituteUniversity of Valladolid - INIAPalenciaSpain
  2. 2.Forestry EngineeringUniversity of ExtremaduraPlasenciaSpain
  3. 3.The Finnish Forest Research InstituteVantaaFinland

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