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Apidologie

pp 1–13 | Cite as

Antibiotic treatment impairs protein digestion in the honeybee, Apis mellifera

  • Esther E. du RandEmail author
  • Christian Stutzer
  • Hannelie Human
  • Christian W. W. Pirk
  • Susan W. Nicolson
Original article

Abstract

Nutritional stress due to habitat transformation and loss is one of several factors contributing to current declines in global bee populations. Bees obtain protein from pollen, which in honeybees is consumed and digested by nurse bees. They then distribute the protein to the rest of the colony in the form of hypopharyngeal gland secretions. Little is known of how efficiently honeybees digest protein. Moreover, antibiotics are used by beekeepers as in-hive treatments for diseases and may interfere with microbial contributions to protein digestion. Caged, newly emerged workers of Apis mellifera scutellata were fed caseinate as protein source, to investigate the effects of protein intake and antibiotic treatment on digestive efficiency. These workers were fed protein:carbohydrate ratios of 1:120, 1:50 and 1:15 or pure sucrose for 9 days. Half the cages received dietary oxytetracycline at a concentration used by beekeepers. Antibiotic exposure did not affect survival or protein consumption. Protein digestive efficiency increased with increasing levels of protein in the diet, although a decrease would have contributed to maintaining nutrient balance. Importantly, we show that antibiotic exposure impaired protein digestive efficiency, especially on low-protein diets. This may be particularly important when colonies are restricted to a single protein deficient source of pollen.

Keywords

Bee nutrition Protein digestion Digestive efficiency Antibiotic Oxytetracycline 

Notes

Acknowledgements

We thank G. Wright and Z. Apostolides for useful discussions.

Authors’ contributions

S.W.N conceived the study. All authors designed the study. E.E.dR, C.S. and H.H. performed the experiments. E.E.dR performed data analysis and wrote the first draft of the manuscript. C.W.W.P. and S.W.N. contributed to revisions and secured funding. All authors gave final approval for publication.

Funding information

This work was supported by the University of Pretoria, including a postdoctoral scholarship to E.E.dR, and by the National Research Foundation of South Africa to S.W.N. and C.W.W.P.

Un traitement antibiotique entrave la digestion des protéines chez Apis mellifera.

nutrition des abeilles / digestion des protéines / antibiotique / oxytétracycline.

Eine Antibiotikabehandlung beeinträchtigt die Eiweißverdauung bei der Honigbiene Apis mellifera .

Bienenernährung / Eiweißverdauung / Antibiotika / Oxytetracycline.

Supplementary material

13592_2019_718_MOESM1_ESM.pdf (683 kb)
ESM 1 (PDF 683 kb)

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Copyright information

© INRA, DIB and Springer-Verlag France SAS, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Zoology and EntomologyUniversity of PretoriaHatfieldSouth Africa
  2. 2.Department of Biochemistry, Genetics and MicrobiologyUniversity of PretoriaHatfieldSouth Africa

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