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Apidologie

pp 1–14 | Cite as

Retained metabolic activity in honey bee collected pollen has implications for pollen digestion and effects on honey bee health

  • Mia McKinstry
  • Sofia R. Prado-Irwin
  • Tara Reyes Adames
  • Jonathan W. SnowEmail author
Original article

Abstract

The mechanisms by which pollen is digested by honey bees are incompletely understood. Potential methods are thought to include pseudogermination, mechanical disruption, enzymatic breakdown, or osmotic shock. Understanding the role of pseudogermination in this process has been hampered by a lack of tools demonstrating retention of metabolic activity in pollen collected by honey bees. Here, we show that pollen collected by honey bees produces reactive oxygen species (ROS) at robust levels upon germination, suggesting that ROS is a suitable marker of this process in pollen. ROS can be readily found in the digestive tract of honey bees and is localized to pollen grains within the lumen. Finally, manipulating pollen levels in the midgut can change ROS levels in the digestive tract. These data provide evidence of retained metabolic activity in bee-collected pollen that lends support to pseudogermination as a mechanism for pollen digestion in honey bees, and points to novel approaches for better understanding of pollen digestion in this species and beyond.

Keywords

ecology honey bee pollen pollinator digestion 

Notes

Acknowledgments

The authors acknowledge Heather Mattila for helpful comments about this study.

Authors’ contributions

MM, SRP, and JWS conceived and designed the experiments. MM, SRP, TRA, and JWS performed experiments and analyzed the data. All authors contributed to the drafting and revision of the article.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13592_2019_703_MOESM1_ESM.docx (22 kb)
ESM 1 (DOCX 22 kb)
13592_2019_703_MOESM2_ESM.docx (22 kb)
ESM 2 (DOCX 168 kb)

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Copyright information

© INRA, DIB and Springer-Verlag France SAS, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Biology DepartmentBarnard CollegeNew YorkUSA

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