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Apidologie

, Volume 50, Issue 5, pp 616–631 | Cite as

Bioactivity of gallic acid–conjugated silica nanoparticles against Paenibacillus larvae and their host, Apis mellifera honeybee

  • Enzo DomínguezEmail author
  • María P. Moliné
  • María S. Churio
  • Valeria B. Arce
  • Daniel O. Mártire
  • Sara N. Mendiara
  • Brenda S. Álvarez
  • Liesel B. Gende
  • Natalia Damiani
Original article

Abstract

The aim of this work was to evaluate antimicrobial activity against Paenibacillus larvae and oral toxicity against workers and larvae of Apis mellifera of gallic acid (GA) and two nanohybrids of GA and silica. Also, the physicochemical, structural, and energetic properties of GA and the nanohybrids were determined through structure–activity relationship (SAR). The minimum inhibitory concentration (MIC) against P. larvae was determined. GA showed MIC values between 62.5 and 125 μg/ml, whereas the nanoparticle functionalized through the GA carboxylic moiety (NP2) showed the best antimicrobial activity with a MIC value of 23 μg GA/ml for four of the five isolates used. SAR analysis showed that electronegativity, chemical hardness, and dipolar moment are reliable estimators of the antimicrobial activity. NP2 showed the lowest toxicity against workers and was innocuous for bee larvae. Therefore, the nanohybrid NP2 was the best antibacterial and resulted in non-toxic against workers and larvae of honeybees, becoming a potentially effective and safe agent for the treatment of American Foulbrood disease.

Keywords

Apis mellifera Paenibacillus larvae gallic acid silica nanohybrid 

Notes

Author Contributions

AVB and MDO synthesized nanohybrids; MSN performed computer analyzes; ABS and MMP collaborated in antimicrobial assays; CMS read and corrected the manuscript; DN and GLB led the work.

Funding information

This work was supported by CONICET, EXA Project No. 779/16 (UNMDP) and CONICET PIP Project No. 0804 to LG.

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Copyright information

© INRA, DIB and Springer-Verlag France SAS, part of Springer Nature 2019

Authors and Affiliations

  • Enzo Domínguez
    • 1
    • 2
    Email author
  • María P. Moliné
    • 1
    • 2
    • 3
  • María S. Churio
    • 3
    • 2
  • Valeria B. Arce
    • 4
    • 2
  • Daniel O. Mártire
    • 5
    • 2
  • Sara N. Mendiara
    • 6
  • Brenda S. Álvarez
    • 1
    • 2
  • Liesel B. Gende
    • 1
    • 2
  • Natalia Damiani
    • 1
    • 2
  1. 1.Centro de Investigación en Abejas Sociales (CIAS), Instituto de Investigaciones en Producción Sanidad y Ambiente (IIPROSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Comisión de Investigaciones Científicas de la provincia de Buenos Aires (CIC), Facultad de Ciencias Exactas y NaturalesUniversidad Nacional de Mar del PlataMar del Plata, Buenos AiresArgentina
  2. 2.Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)CABAArgentina
  3. 3.Departamento de Química, Facultad de Ciencias Exactas y Naturales (FCEyN). Instituto de Investigaciones Físicas de Mar del Plata (IFIMAR), CONICETUniversidad Nacional de Mar del PlataMar del Plata, Buenos AiresArgentina
  4. 4.Centro de Investigaciones Ópticas (CIOp)CONICET - CIC - Universidad Nacional de La Plata (UNLP)Manuel B. Gonnet, Buenos AiresArgentina
  5. 5.Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), CONICETUniversidad Nacional de La Plata (UNLP)La Plata, Buenos AiresArgentina
  6. 6.Departamento de Química, Facultad de Ciencias Exactas y Naturales (FCEyN)Universidad Nacional de Mar del Plata (UNMdP)Mar del Plata, Buenos AiresArgentina

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