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Apidologie

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Estimating the density of honey bee (Apis mellifera) colonies using trapped drones: area sampled and drone mating flight distance

  • Patsavee UtaipanonEmail author
  • Michael J. Holmes
  • Nadine C. Chapman
  • Benjamin P. Oldroyd
Original article
  • 13 Downloads

Abstract

Reliable information on Western honey bee colony density can be important in a variety of contexts including biosecurity responses, determining the sufficiency of pollinators in an agroecosystem and in determining the impacts of feral honey bees on ecosystems. Indirect methods for estimating colony density based on genetic analysis of sampled males are more feasible and cost efficient than direct observation in the field. Microsatellite genotypes of drones caught using Williams drone trap are used to identify the number of colonies (queens) that contributed drones to a mating lek. From the number of colonies, the density of colonies can be estimated based on assumptions about the area from which drones are drawn. This requires reliable estimates of drone flight distance. We estimated average minimum flight distance of drones from feral colonies along two 7-km transects in Southern NSW, Australia. We found that drones from feral colonies flew at least 3.5 km to drone traps. We then determined that the maximum distance that drones flew from a focal colony to a trap was 3.75 km. We conclude that a drone trap samples an area of 44 km2, and that this area should be used to convert estimated colony numbers to colony densities. This area is much greater than has been previously assumed. The densities of honey bee colonies in Grong Grong and Currawarna, NSW, are 1.38–2.73 and 1.31–3.06 colonies/km2 respectively.

Keywords

drone flight range colony density mating behaviour feral honey bee 

Notes

Acknowledgements

We thank Frank Malfroy and Jenny Douglas for a very nice dinner and extraordinary logistical help and advice at the Lyndhurst site. Madeleine Beekman helped with drone trapping. Lea Ubdulkhalek helped with sample processing.

Funding information

This project was financially supported by AgriFutures Australia, though funding from the Australian Government Department of Agriculture as part of its Rural R&D for Profit program, with further support from Horticulture Innovation Australia, the Almond Board of Australia, Lucerne Australia, Costa Group, and Raspberries and Blackberries Australia.

Supplementary material

13592_2019_671_MOESM1_ESM.xlsx (14 kb)
Supplementary table S1. Distribution of drones among sites from families in which drone frequency was above the 90th percentile (XLSX 14 kb)
13592_2019_671_MOESM2_ESM.xlsx (382 kb)
Supplementary table S2.1. Drone genotypes from transect A. Supplementary Table S2.2 Drone genotypes from transect B (XLSX 381 kb)
13592_2019_671_MOESM3_ESM.xlsx (17 kb)
Supplementary table S3. Genotypes of drone pupa collect from the experimental colony, reconstructed queen genotype, and genotypes of drones collected at 4000 m far from the experimental hive. (XLSX 17 kb)
13592_2019_671_MOESM4_ESM.mp4 (6.7 mb)
Supplementary video file. Catching drones in a Williams drone trap (MP4 6888 kb)

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Copyright information

© INRA, DIB and Springer-Verlag France SAS, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Behaviour and Genetics of Social Insects Laboratory, Ecology and EvolutionUniversity of SydneySydneyAustralia

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