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Apidologie

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Genetic origins of honey bees (Apis mellifera) on Kangaroo Island and Norfolk Island (Australia) and the Kingdom of Tonga

  • Nadine C. Chapman
  • Jiani Sheng
  • Julianne Lim
  • Samuel F. Malfroy
  • Brock A. Harpur
  • Amro Zayed
  • Michael H. Allsopp
  • Thomas E. Rinderer
  • John M. K. Roberts
  • Emily J. Remnant
  • Benjamin P. Oldroyd
Original article
  • 21 Downloads

Abstract

We examine the origin of honey bee (Apis mellifera) populations in Kangaroo Island (Australia), Norfolk Island (Australia) and the Kingdom of Tonga using a highly polymorphic mitochondrial DNA region and a panel of 37 single nucleotide polymorphisms that assigns ancestry to three evolutionary lineages: Eastern Europe, Western Europe and Africa. We also examine inbreeding coefficients and genetic variation using microsatellites and mitochondrial sequencing. The honey bees of Kangaroo Island have a high proportion of Eastern European ancestry (90.2%), consistent with claims that they are of the subspecies A. m. ligustica. The honey bees of Norfolk Island also had a majority of ancestry from Eastern Europe (73.1%) with some contribution from Western Europe (21.2%). The honey bees of Tonga are mainly of Western European (70.3%) origin with some Eastern European ancestry (27.4%). Despite the suspected severe bottlenecks experienced by these island population, inbreeding coefficients were low.

Keywords

Apis mellifera ancestry assignment population genetics isolated populations single nucleotide polymorphisms 

Notes

Acknowledgements

We thank Peter Davis for collecting bees from Kangaroo Island, Bruce White and Lamorna Osborne for collecting bees from Tonga and the beekeepers of Norfolk Island for providing access to their colonies to SFM. We thank two anonymous reviewers for their helpful comments which improved the manuscript.

Author contributions

NCC, BPO, SFM, JMKR and EJR designed the experiment. NCC and JS extracted DNA and performed genotyping. NCC performed data analysis. AZ and BAH provided SNPs and data. NCC, BPO, SFM, JMKR, EJR, JL, MHA and TER provided samples. NCC and BPO wrote the paper, with suggestions from co-authors. All authors read and approved the article prior to publication.

Funding information

This project was supported by Rural Industries Research and Development Corporation (AgriFutures) Australia PRJ-007774 (BPO), Science and Innovation Award for Young People in Agriculture, Fisheries and Forestry: CSIRO Biosecurity Flagship 2015 (NCC), Natural Sciences and Engineering Research Council Canada Discovery grant (AZ), Ontario Ministry of Research and Innovation’s Early Researcher Award (AZ), Natural Sciences and Engineering Research Council Canada Postdoctoral Fellowship (BAH), Australian and Pacific Science Foundation grant APSF15-02 (EJR), the Australian Government and the Norfolk Island Administration (SFM) and Rural Industries Research and Development Corporation (AgrFutures) Australia PRJ-008540 (JMKR).

Supplementary material

13592_2018_615_MOESM1_ESM.docx (19 kb)
ESM 1. (DOCX 18 kb)

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Copyright information

© INRA, DIB and Springer-Verlag France SAS, part of Springer Nature 2019

Authors and Affiliations

  • Nadine C. Chapman
    • 1
  • Jiani Sheng
    • 1
  • Julianne Lim
    • 1
  • Samuel F. Malfroy
    • 2
  • Brock A. Harpur
    • 3
    • 4
  • Amro Zayed
    • 3
  • Michael H. Allsopp
    • 5
  • Thomas E. Rinderer
    • 6
  • John M. K. Roberts
    • 7
  • Emily J. Remnant
    • 1
  • Benjamin P. Oldroyd
    • 1
  1. 1.School of Life and Environmental Science, Behaviour and Genetics of Social Insects LaboratoryUniversity of SydneySydneyAustralia
  2. 2.Australian Government Department of Agriculture and Water ResourcesCanberraAustralia
  3. 3.Department of BiologyYork UniversityTorontoCanada
  4. 4.Donnelly Centre for Cellular and Biomolecular ResearchUniversity of TorontoTorontoCanada
  5. 5.ARC-Plant Protection Research InstituteStellenboschSouth Africa
  6. 6.Honey Bee Breeding, Genetics and Physiology Research LaboratoryUSDA-ARSBaton RougeUSA
  7. 7.Health and Biosecurity, Commonwealth Scientific and Industrial Research OrganisationBlack MountainAustralia

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