, Volume 48, Issue 5, pp 648–656 | Cite as

Genetic diversity of Varroa destructor parasitizing Apis mellifera unicolor in Madagascar

  • Johanna CLÉMENCET
  • Adrien SPECK
  • Bernard REYNAUD
  • Hélène DELATTE
Original article


Varroa destructor is an invasive alien species that has been reported parasitizing the endemic honey bee of Madagascar, Apis mellifera unicolor, since 2010. Studying its nuclear genetic diversity and structure was our main goal. Using 11 microsatellite loci and 344 mites collected from 12 apiaries, we observed a low genetic diversity, with only 8 multilocus genotypes (MLG) identified. V. destructor populations form a single genetic cluster, clonal richness ranged from 0.02 to 0.20, and number of MLG within apiaries varied between one and six MLGs. About 69.5% of the mites analyzed harbored the same genotype (100%, homozygous), and 23.3% had a genotype differing by a single allele. The overall low diversity observed suggests one or multiple introductions of similar genotypes. The greater abundance of MLGs in High Land apiaries (eight MLGs) than on the east coast (two MLGs) and the presence of particular MLGs in High Land apiaries favor the hypothesis that V. destructor has been first introduced close to the international airport, and then spread to other regions by commercial exchanges.


microsatellite multilocus genotypes Varroa destructor Madagascar 



We would like to thank Niaina Andrianaivoariseta and Dimby Razafindrazaka for their help with data collection. We are grateful to the Malagasy beekeepers who participated in the study. This work is part of the PhD of Henriette Rasolofoarivao, recipient of a grant of CIRAD-AIRD-Sud. Fieldwork had been partly funded by CIRAD, the Enlargement and sustainability of the Plant Protection Network (ePRPV) project supported by the European Union, the French government, and Réunion collectivities.

Authors’ contributions

HD and JC designed protocols; HR did the fieldwork; AS and HR did the lab work; HR, HD, and JC did the analyses; and HR, HD, JC, BR, and LHRR interacted for writing the manuscript.

Supplementary material

13592_2017_509_MOESM1_ESM.pdf (98 kb)
Fig. S1 (PDF 97 kb)


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Copyright information

© INRA, DIB and Springer-Verlag France 2017

Authors and Affiliations

    • 1
    • 2
  • Johanna CLÉMENCET
    • 3
  • Adrien SPECK
    • 1
    • 2
  • Bernard REYNAUD
    • 1
  • Hélène DELATTE
    • 1
  1. 1.CIRAD, UMR PVBMTSaint PierreFrance
  2. 2.Département d’Entomologie, Faculté de SciencesUniversité d’AntananarivoAntananarivoMadagascar
  3. 3.Université de La Réunion, UMR PVBMTSaint Denis cedex 9France

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