Apidologie

, Volume 46, Issue 3, pp 346–358 | Cite as

Mitochondrial DNA of Euglossa iopoecila (Apidae, Euglossini) reveals two distinct lineages for this orchid bee species endemic to the Atlantic Forest

  • Rafael E. S. Penha
  • Maria C. Gaglianone
  • Fernanda S. Almeida
  • Samuel V. Boff
  • Silvia H. Sofia
Original article
First Online:
Received:
Revised:
Accepted:

Abstract

This study analysed the population genetic structure of Euglossa iopoecila, an orchid bee species endemic to the Atlantic Forest which shows a variation in the colour of its metallic integument across its distribution. Our analyses were based on microsatellite and mitochondrial markers. From ten microsatellite loci surveyed, six are described herein for the first time. Mitochondrial markers were obtained by sequencing 651 bp of Cytb gene. Bees were collected from six Atlantic Forest remnants distributed along the Serra do Mar Biodiversity Corridor, which is one of the largest remnants of dense forest within the Atlantic Forest of southern and south-eastern Brazil. The microsatellite markers showed lower levels of genetic structure than those found for mitochondrial markers. Our findings based on mitochondrial markers indicate the existence of at least two different evolutionarily significant units of E. iopoecila populations along the Serra do Mar Biodiversity Corridor.

Keywords

euglossine genetic diversity microsatellite markers mtDNA evolutionarily significant units 
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Notes

Acknowledgements

The authors thank CNPq, CAPES/Procad (158/2007), Fundação Araucária and FBPN for financial support; CAPES for a scholarship awarded to R.E.S. Penha; Maria C. Arias and Elaine Françoso (USP) for the valuable support in constructing the microsatellite-enriched library; ICMBio and IAP, Salto Morato Reserve and IF-São Paulo for the collecting permits; the staff of the State Parks of São Sebastião, Ilhabela and UB (Picinguaba) and the Salto Morato Reserve for providing infrastructure and for their assistance during sampling. Silvia H. Sofia and Maria C. Gaglianone are research fellows from CNPq. Finally, the authors would like to thank the two anonymous reviewers for their valuable comments and suggestions.

Supplementary material

13592_2014_329_MOESM1_ESM.pdf (519 kb)
ESM 1 (PDF 519 kb)

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Copyright information

© INRA, DIB and Springer-Verlag France 2014

Authors and Affiliations

  • Rafael E. S. Penha
    • 1
    • 2
  • Maria C. Gaglianone
    • 3
  • Fernanda S. Almeida
    • 1
  • Samuel V. Boff
    • 4
  • Silvia H. Sofia
    • 1
  1. 1.Laboratório de Genética e Ecologia Animal, Departamento de Biologia GeralUniversidade Estadual de LondrinaLondrinaBrazil
  2. 2.Programa de Pós-Graduação em Genética e Biologia MolecularUniversidade Estadual de LondrinaLondrinaBrazil
  3. 3.Laboratório de Ciências Ambientais, Programa de Pós-Graduação em Ecologia e Recursos NaturaisUniversidade Estadual do Norte Fluminense Darcy RibeiroCampos dos GoytacazesBrazil
  4. 4.General ZoologyMartin-Luther-Universität Halle-WittenbergHalleGermany

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