, Volume 44, Issue 5, pp 519–521

A scientific note on diploid males in a reproductive event of a eusocial bee

  • Charles Fernando dos Santos
  • Cristiano Menezes
  • Vera Lucia Imperatriz-Fonseca
  • Maria Cristina Arias
Scientific note


bees diploid male Hymenoptera inbreeding 


  1. Alves, D.A., Imperatriz-Fonseca, V.L., Francoy, T.M., Santos-Filho, P.S., Billen, J., Wenseleers, T. (2011) Successful maintenance of a stingless bee population despite a severe genetic bottleneck. Conserv. Genet. 12, 647–658CrossRefGoogle Scholar
  2. Borges, A.A., Ferreira-Caliman, M.J., Nascimento, F.S., Campos, L.A.O., Tavares, M.G. (2012) Characterization of cuticular hydrocarbons of diploid and haploid males, workers and queens of the stingless bee Melipona quadrifasciata. Insect. Soc.. doi:10.1007/s00040-012-0242-x
  3. Brito, R.M., Francisco, F.O., Domingues-Yamada, A.M.T., Gonçalves, P.H.P., Pioker, F.C., Soares, A.E.E., Arias, M.C. (2009) Characterization of microsatellite loci of Tetragonisca angustula (Hymenoptera, Apidae, Meliponini). Conserv. Genet. Resour. 1, 183–187CrossRefGoogle Scholar
  4. Cameron, E.C., Franck, P., Oldroyd, B.P. (2004) Genetic structure of nest aggregations and drone congregations of the southeast Asian stingless bee Trigona collina. Mol. Ecol. 13, 2357–2364PubMedCrossRefGoogle Scholar
  5. Cook, J.M., Crozier, R.H. (1995) Sex determination and population biology in the Hymenoptera. Trends Ecol. Evol. 10, 281–286PubMedCrossRefGoogle Scholar
  6. Cournault, L., Aron, S. (2009) Diploid males, diploid sperm production, and triploid females in the ant Tapinoma erraticum. Naturwissenschaften 96, 1393–1400PubMedCrossRefGoogle Scholar
  7. Darvill, B., Lepais, O., Woodall, L.C., Goulson, D. (2012) Triploid bumblebees indicate a direct cost of inbreeding in fragmented populations. Mol. Ecol. 21, 3988–3995PubMedCrossRefGoogle Scholar
  8. Francini, I.B., Nunes-Silva, C.G., Carvalho-Zilse, G.A. (2012) Diploid male production of two Melipona bees (Hymenoptera: Apidae). Psyche. doi:10.1155/2012/484618
  9. Kerr, W.E., Zucchi, R., Nakadaira, J.T., Butolo, J.E. (1962) Reproduction in the social bees (Hymenoptera: Apidae). J. New York Entomol. Soc. 70, 265–276Google Scholar
  10. Konovalov, D.A., Manning, C., Henshaw, M.T. (2004) Kingroup: a program for pedigree relationship reconstruction and kin group assignments using genetic markers. Mol. Ecol. Notes 4, 779–782CrossRefGoogle Scholar
  11. Kraus, F.B., Weinhold, S., Moritz, R.F.A. (2008) Genetic structure of drone congregations of the stingless bee Scaptotrigona mexicana. Insect. Soc. 55, 22–27CrossRefGoogle Scholar
  12. Liebert, A.E., Johnson, R.N., Switz, G.T., Starks, P.T. (2004) Triploid females and diploid males: underreported phenomena in Polistes wasps? Insect. Soc. 51, 205–211Google Scholar
  13. Nogueira-Neto, P. (1954) Notas bionômicas sobre meliponíneos III—sobre a enxameagem. Arq. Mus. Nac. 42, 419–452Google Scholar
  14. Paxton, R.J. (2000) Genetic structure of colonies and a male aggregation in the stingless bee Scaptotrigona postica, as revealed by microsatellite analysis. Insect. Soc. 47, 63–69CrossRefGoogle Scholar
  15. Peters, J.M., Queller, D.C., Imperatriz-Fonseca, V.L., Roubik, D.W., Strassmann, J.E. (1999) Mate number, kin selection, and social conflicts in stingless bees and honey bees. Proc. Roy. Soc. Lond. B 266, 379–384CrossRefGoogle Scholar
  16. Sommeijer, M.J., Bruijn, L.L.M. (1995) Drone congregations apart from the nest in Melipona favosa. Insect. Soc. 42, 123–127CrossRefGoogle Scholar
  17. Van Veen, J.W., Sommeijer, M.J. (2000) Observations on gynes and drones around nuptial flights in the stinglees bees Tetragonisca angustula and Melipona beecheii (Hymenoptera, Apidae). Apidologie 31, 47–54CrossRefGoogle Scholar
  18. Van Veen, J.W., Sommeijer, M.J., Meeuwsen, F. (1997) Behaviour of drones in Melipona (Apidae, Meliponinae). Insect. Soc. 44, 435–447CrossRefGoogle Scholar
  19. Walsh, P.S., Metzger, D.A., Higuchi, R. (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10, 506–513PubMedGoogle Scholar
  20. Whiting, P.W. (1943) Multiple alleles in complementary sex determination of Habrobracon. Genetics 28, 365–382PubMedGoogle Scholar
  21. Zayed, A., Packer, L. (2005) Complementary sex determination substantially increases extinction proneness of haplodiploid populations. Proc. Natl. Acad. Sci. 102, 10742–10746PubMedCrossRefGoogle Scholar

Copyright information

© INRA, DIB and Springer-Verlag France 2013

Authors and Affiliations

  • Charles Fernando dos Santos
    • 1
    • 2
  • Cristiano Menezes
    • 3
  • Vera Lucia Imperatriz-Fonseca
    • 2
    • 4
  • Maria Cristina Arias
    • 5
  1. 1.Departamento de Zoologia, IBUniversidade de São PauloSão PauloBrazil
  2. 2.Laboratório de Abelhas, Departamento de Ecologia, IBUniversidade de São PauloSão PauloBrazil
  3. 3.Empresa Brasileira de Pesquisa Agropecuária–Amazônia OrientalBelémBrazil
  4. 4.Universidade Federal Rural do SemiáridoMossoróBrazil
  5. 5.Departamento de Genética e Biologia Reprodutiva, IBUniversidade de São PauloSão PauloBrazil

Personalised recommendations