Apidologie

, Volume 44, Issue 2, pp 188–197 | Cite as

Microsatellite analysis in museum samples reveals inbreeding before the regression of Bombus veteranus

  • Kevin Maebe
  • Ivan Meeus
  • Jafar Maharramov
  • Patrick Grootaert
  • Denis Michez
  • Pierre Rasmont
  • Guy Smagghe
Original article

Abstract

The worldwide decline of pollinators is an emerging threat and is a matter both for ecological and economic concerns. Population genetics can be used to correlate bumblebee decline with genetic parameters. In order to do so, historical data are needed. We used eight microsatellite loci to genotype pinned museum specimens of the declining bumblebee Bombus veteranus. Bumblebee samples were collected spanning a period of three decades (1895–1923). We detected low genetic diversity and inbreeding in the samples collected during the respective time periods. Inbreeding was also confirmed by the occurrence of sterile diploid males. Based on the data obtained, we speculate that low genetic diversity and inbreeding did not directly result in the collapse of B. veteranus in Belgium. However, inbreeding might still play an indirect role in the decline of bumblebee populations because of the appearance of diploid males and because a low HE might reduce the capacity to react to the drivers of bumblebee decline.

Keywords

microsatellites ancient DNA museum collections genetic diversity bumblebee decline 

Notes

Acknowledgments

This project was supported by the Special Research Fund of Ghent University, the Fund for Scientific Research-Flanders (FWO-Vlaanderen), and the Fonds de la Recherche Scientifique–Fonds de la Recherche Fondamentale Collective (FNRS-FRFC) (FRFC convention no. 2.4618.12).

Bombus veteranus :l’analyse de microsatellites de cette espèce à partir d’échantillons d’un museum révèle une consanguinité avant leur régression.

Microsatellites / ADN ancien / collections muséographiques / diversité génétique / déclin des bourdons

Mikrosatelliten-Analyse von Museumsproben verrät das Auftreten von Inzucht bereits vor dem Rückgang vonBombus veteranus.

Mikrosatelliten / ancient DNA / Museumssammlungen / geneRückgang von Hummeln / Rückgang von Hummeln

Supplementary material

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Copyright information

© INRA, DIB and Springer-Verlag France 2012

Authors and Affiliations

  • Kevin Maebe
    • 1
  • Ivan Meeus
    • 1
  • Jafar Maharramov
    • 1
  • Patrick Grootaert
    • 2
  • Denis Michez
    • 3
  • Pierre Rasmont
    • 3
  • Guy Smagghe
    • 1
  1. 1.Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience EngineeringGhent UniversityGhentBelgium
  2. 2.Royal Belgian Institute of Natural Sciences, Department EntomologyBrusselsBelgium
  3. 3.Laboratoire de ZoologyUniversité de Mons-HainautMonsBelgium

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