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Apidologie

, Volume 43, Issue 6, pp 731–736 | Cite as

Genome size diversity in stingless bees (Hymenoptera: Apidae, Meliponini)

  • Mara Garcia Tavares
  • Carlos Roberto Carvalho
  • Fernanda Aparecida Ferrari Soares
  • Lucio Antonio de Oliveira Campos
Original article

Abstract

The first studies on the genome size of stingless bee species showed a range from 0.27 pg (Melipona subnitida and Melipona quadrifasciata) to 1.38 pg (Melipona capixaba). Considering this variation, we quantified the DNA content of 26 species of Meliponini, in order to provide input for future comparative studies in this tribe. Haploid genome size (1C) estimates, using flow cytometry analyses (FCM), ranged from 0.26 ± 0.003 pg (Paratrigona subnuda) to 0.98 ± 0.023 pg (Melipona flavolineata), with an average of 0.54 ± 0.17 pg. FCM analyses also demonstrated a small difference in the haploid genome size between males and females of the same species, with the males generally having a smaller genome than females. Our data also evidenciated that variations in the genome size of stingless bees do not correlate with changes in chromosome number and that in some genera the DNA content is more variable than in others.

Keywords

C-value DNA content flow cytometry meliponines 

Notes

Acknowledgments

We are grateful to Dr. Leonardo Lopes Bhering for his statistical assistance with GENES Program and to all researches and bee keepers that kindly provided the specimens analyzed. This work was supported by the Brazilian agency FAPEMIG (Process number: APQ 218 00564-08).

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Copyright information

© INRA, DIB and Springer-Verlag, France 2012

Authors and Affiliations

  • Mara Garcia Tavares
    • 1
  • Carlos Roberto Carvalho
    • 1
  • Fernanda Aparecida Ferrari Soares
    • 1
  • Lucio Antonio de Oliveira Campos
    • 1
  1. 1.Departamento de Biologia GeralUniversidade Federal de ViçosaViçosaBrazil

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