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Apidologie

, Volume 43, Issue 6, pp 698–714 | Cite as

Is flower selection influenced by chemical imprinting to larval food provisions in the generalist bee Osmia bicornis (Megachilidae)?

  • Heidi E. M. DobsonEmail author
  • Manfred Ayasse
  • Katherine A. O’Neal
  • Jesse A. Jacka
Original article

Abstract

To investigate whether flower selection in polylectic solitary bees is modulated by chemical imprinting to nest provisions, larvae of Osmia bicornis (L.) were reared on either Brassica napus L. (Brassicaceae) or Onobrychis viciifolia Scop. (Fabaceae). Flower preferences by adults were evaluated in multiple-choice behavioral tests based on visit number and duration, and flowers selected in the first three visits; data were compared to control bees from the wild. Females reared on B. napus showed only subtle increases in selection for this species, which was highly attractive to both control and experimental bees, masking any effects of imprinting; however, in the first three visits, experimental bees tended to select B. napus more frequently and consistently than controls. Bees reared on O. viciifolia were few and mostly males, which tended to visit this species more than controls. Rearing larvae on either plant affected bee attraction to other plant species. Overall, the data do not provide clear evidence of imprinting, but suggest that rearing bees on a single plant can both directly and indirectly affect flower selection by adults.

Keywords

solitary bees polylecty flower selection imprinting larval food 

Notes

Acknowledgments

We deeply thank staff and faculty at the University of Vienna for all their support and assistance, particularly the Department of Evolutionary Biology, Prof. Dr. H.F. Paulus and Eva Zellinger, Dr. H. Pechhacker of the Institut für Bienenkunde for setting up bee tents over specially planted fields, the Botanical Garden for permission to rear bees and collect flowers, and the Zoological Institute for greenhouse space to conduct tests. We are grateful to two anonymous reviewers who provided very helpful and thoughtful comments to improve our manuscript. Many thanks are given to the following for funding various parts of this project: M.J. Murdock Charitable Trust College Science Research Program through Whitman College (KAO and HEMD), Whitman Science Summer Research (JAJ and HEMD), Whitman College ASID funds (HEMD), and Fellowship for Foreign Research from the University of Vienna (HEMD).

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Copyright information

© INRA, DIB and Springer-Verlag, France 2012

Authors and Affiliations

  • Heidi E. M. Dobson
    • 1
    Email author
  • Manfred Ayasse
    • 2
  • Katherine A. O’Neal
    • 1
    • 3
  • Jesse A. Jacka
    • 1
    • 4
  1. 1.Department of BiologyWhitman CollegeWalla WallaUSA
  2. 2.Institute of Experimental EcologyUniversity of UlmUlmGermany
  3. 3.Las VegasUSA
  4. 4.Colorado SpringsUSA

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