Advertisement

Apidologie

, Volume 43, Issue 5, pp 587–589 | Cite as

A scientific note on Israeli acute paralysis virus infection of Eastern honeybee Apis cerana and vespine predator Vespa velutina

  • Orlando Yañez
  • Huo-Qing Zheng
  • Fu-Liang Hu
  • Peter Neumann
  • Vincent DietemannEmail author
Scientific Note

The Israeli acute paralysis virus (IAPV; Maori et al. 2007; de Miranda et al. 2010) is widespread in Western honeybees (Apis mellifera) for whom it can be a marker of colony losses (Cox-Foster et al. 2007). It has also been found in other Hymenoptera (e.g., Vespula vulgaris; Singh et al. 2010), but the ability of the virus to replicate in such alternative hosts still has to be confirmed, as has been shown recently for the ectoparasite Varroa destructor (Di Prisco et al. 2011). We investigated whether IAPV can be found in Western and Eastern (Apis cerana) honeybees and in their hornet predator Vespa velutina in China and whether this virus is able to replicate in these organisms. Positive-strand RNA virus replication requires the production of negative-strand RNA replicative intermediates. Detection of negative-strand RNA of IAPV in A. cerana and V. velutina would strongly support their function as alternative hosts of this virus.

Eastern honeybee workers (N = 180) were sampled from...

Keywords

Apis mellifera Apis cerana Vespa velutina IAPV virus honeybee 

Notes

Acknowledgements

We wish to thank Xiao-Ling Su and Jin-Lian Zhang for their help in the field, Qi-Yun Hua for logistical assistance, Zhang-Gen Yu for access to his apiary for sampling and Benjamin Dainat for laboratory support. Financial support was provided to OY and PN by the COST-funded project VIVA and to VD by the Qian-Tang River expert programme. The funding agencies were not involved in study design; in the collection, analysis and interpretation of data; in the writing of the report; and in the decision to submit the paper for publication.

References

  1. Anderson, D.L. (1991) Kashmir bee virus—a relatively harmless virus of honey bee colonies. Am. Bee J. 131, 767–770Google Scholar
  2. Boncristiani, H.F., Di Prisco, G., Pettis, J.S., Hamilton, M., Chen, Y.P. (2009) Molecular approaches to the analysis of deformed wing virus replication and pathogenesis in the honey bee, Apis mellifera. Virol. J. 6, 221PubMedCrossRefGoogle Scholar
  3. Celle, O., Blanchard, P., Olivier, V., Schurr, F., Cougoule, N., Faucon, J.P., Ribière, M. (2008) Detection of chronic bee paralysis virus (CBPV) genome and its replicative RNA form in various hosts and possible ways of spread. Virus Res. 133, 280–284PubMedCrossRefGoogle Scholar
  4. Cox-Foster, D.L., Conlan, S., Holmes, E.C., Palacios, G., Evans, J.D., Moran, N.A., Quan, P.L., Briese, T., Hornig, M., Geiser, D.M., Martinson, V., Vanengelsdorp, D., Kalkstein, A.L., Drysdale, A., Hui, J., Zhai, J., Cui, L., Hutchison, S.K., Simons, J.F., Egholm, M., Pettis, J.S., Lipkin, W.I. (2007) A metagenomic survey of microbes in honey bee colony collapse disorder. Science 318, 283–287PubMedCrossRefGoogle Scholar
  5. Dainat, B., Ken, T., Berthoud, H., Neumann, P. (2009) The ectoparasitic mite Tropilaelaps mercedesae (Acari: Laelapidae) as a vector of honeybee viruses. Insect Soc. 56, 40–43CrossRefGoogle Scholar
  6. de Miranda, J.R., Cordoni, G., Budge, G. (2010) The acute bee paralysis virus–Kashmir bee virus–Israeli acute paralysis virus complex. J. Invertebr. Pathol. 103, 30–47CrossRefGoogle Scholar
  7. Di Prisco, G., Pennacchio, F., Emilio, C., Boncristiani, H., Evans, J.D., Chen, Y.P. (2011) Varroa destructor is an effective vector of Israeli acute paralysis virus in honey bees, Apis mellifera. J. Gen. Virol. 92, 151–155PubMedCrossRefGoogle Scholar
  8. Gauthier, L., Tentcheva, D., Tournaire, M., Dainat, B., Cousserans, F., Colin, M.E., Bergoin, M. (2007) Viral load estimation in asymptomatic honey bee colonies using the quantitative RT-PCR technique. Apidologie 38, 426–435CrossRefGoogle Scholar
  9. Genersch, E., Yue, C., Fries, I., de Miranda, J.R. (2006) Detection of Deformed wing virus, a honey bee viral pathogen, in bumble bees (Bombus terrestris and Bombus pascuorum) with wing deformities. J. Invertebr. Pathol. 91, 61–63PubMedCrossRefGoogle Scholar
  10. Maori, E., Lavi, S., Mozes-Koch, R., Gantman, Y., Peretz, Y., Edelbaum, O., Tanne, E., Sela, I. (2007) Isolation and characterization of Israeli acute paralysis virus, a dicistrovirus affecting honeybees in Israel: evidence for diversity due to intra- and inter-species recombination. J. Gen. Virol. 88, 3428–3438PubMedCrossRefGoogle Scholar
  11. Shen, M., Cui, L., Ostiguy, N., Cox-Foster, D. (2005) Intricate transmission routes and interactions between picorna-like viruses (Kashmir bee virus and Sacbrood virus) with the honeybee host and the parasitic varroa mite. J. Gen. Virol. 86, 2281–2289PubMedCrossRefGoogle Scholar
  12. Singh, R., Levitt, A.L., Rajotte, E.G., Holmes, E.C., Ostiguy, N., Vanengelsdorp, D., Lipkin, W.I., Depamphilis, C.W., Toth, A.L., Cox-Foster, D.L. (2010) RNA viruses in hymenopteran pollinators: evidence of inter-taxa virus transmission via pollen and potential impact on non-Apis hymenopteran species. PLoS ONE 5, e14357. doi: 10.1371/journal.pone.0014357 PubMedCrossRefGoogle Scholar
  13. Woolhouse, M.E.J., Taylor, L.H., Haydon, D.T. (2001) Population biology of multihost pathogens. Science 292, 1109–1112PubMedCrossRefGoogle Scholar
  14. Yue, C., Genersch, E. (2005) RT-PCR analysis of Deformed wing virus (DWV) in honey bees (Apis mellifera) and mites (Varroa destructor). J. Gen. Virol. 86, 3419–3424PubMedCrossRefGoogle Scholar

Copyright information

© INRA, DIB and Springer-Verlag, France 2012

Authors and Affiliations

  • Orlando Yañez
    • 1
  • Huo-Qing Zheng
    • 2
  • Fu-Liang Hu
    • 2
  • Peter Neumann
    • 1
    • 3
  • Vincent Dietemann
    • 1
    • 4
    Email author
  1. 1.Swiss Bee Research Centre, Federal Department of Economic Affairs EVDResearch Station Agroscope Liebefeld-Posieux ALPBernSwitzerland
  2. 2.College of Animal SciencesZhejiang UniversityHangzhouChina
  3. 3.Department of Zoology and EntomologyRhodes UniversityGrahamstownSouth Africa
  4. 4.Social Insect Research Group, Zoology and Entomology DepartmentUniversity of PretoriaPretoriaSouth Africa

Personalised recommendations