A scientific note on Israeli acute paralysis virus infection of Eastern honeybee Apis cerana and vespine predator Vespa velutina
- 383 Downloads
The Israeli acute paralysis virus (IAPV; Maori et al. 2007; de Miranda et al. 2010) is widespread in Western honeybees (Apis mellifera) for whom it can be a marker of colony losses (Cox-Foster et al. 2007). It has also been found in other Hymenoptera (e.g., Vespula vulgaris; Singh et al. 2010), but the ability of the virus to replicate in such alternative hosts still has to be confirmed, as has been shown recently for the ectoparasite Varroa destructor (Di Prisco et al. 2011). We investigated whether IAPV can be found in Western and Eastern (Apis cerana) honeybees and in their hornet predator Vespa velutina in China and whether this virus is able to replicate in these organisms. Positive-strand RNA virus replication requires the production of negative-strand RNA replicative intermediates. Detection of negative-strand RNA of IAPV in A. cerana and V. velutina would strongly support their function as alternative hosts of this virus.
Eastern honeybee workers (N = 180) were sampled from...
KeywordsApis mellifera Apis cerana Vespa velutina IAPV virus honeybee
We wish to thank Xiao-Ling Su and Jin-Lian Zhang for their help in the field, Qi-Yun Hua for logistical assistance, Zhang-Gen Yu for access to his apiary for sampling and Benjamin Dainat for laboratory support. Financial support was provided to OY and PN by the COST-funded project VIVA and to VD by the Qian-Tang River expert programme. The funding agencies were not involved in study design; in the collection, analysis and interpretation of data; in the writing of the report; and in the decision to submit the paper for publication.
- Anderson, D.L. (1991) Kashmir bee virus—a relatively harmless virus of honey bee colonies. Am. Bee J. 131, 767–770Google Scholar
- Cox-Foster, D.L., Conlan, S., Holmes, E.C., Palacios, G., Evans, J.D., Moran, N.A., Quan, P.L., Briese, T., Hornig, M., Geiser, D.M., Martinson, V., Vanengelsdorp, D., Kalkstein, A.L., Drysdale, A., Hui, J., Zhai, J., Cui, L., Hutchison, S.K., Simons, J.F., Egholm, M., Pettis, J.S., Lipkin, W.I. (2007) A metagenomic survey of microbes in honey bee colony collapse disorder. Science 318, 283–287PubMedCrossRefGoogle Scholar
- Maori, E., Lavi, S., Mozes-Koch, R., Gantman, Y., Peretz, Y., Edelbaum, O., Tanne, E., Sela, I. (2007) Isolation and characterization of Israeli acute paralysis virus, a dicistrovirus affecting honeybees in Israel: evidence for diversity due to intra- and inter-species recombination. J. Gen. Virol. 88, 3428–3438PubMedCrossRefGoogle Scholar
- Singh, R., Levitt, A.L., Rajotte, E.G., Holmes, E.C., Ostiguy, N., Vanengelsdorp, D., Lipkin, W.I., Depamphilis, C.W., Toth, A.L., Cox-Foster, D.L. (2010) RNA viruses in hymenopteran pollinators: evidence of inter-taxa virus transmission via pollen and potential impact on non-Apis hymenopteran species. PLoS ONE 5, e14357. doi: 10.1371/journal.pone.0014357 PubMedCrossRefGoogle Scholar