, 42:642 | Cite as

Detection and identification of Lactobacillus bacteria found in the honey stomach of the giant honeybee Apis dorsata

  • Naser Tajabadi
  • Makhdzir Mardan
  • Mohd Yazid Abdul Manap
  • Mustafa Shuhaimi
  • Amir Meimandipour
  • Leila Nateghi
Original article


This is the first assay that describes the isolation and identification of strains and species of Lactobacillus from the honey stomach of the Asiatic giant honeybee, Apis dorsata. Samples of honeybees were collected from A. dorsata colonies in different bee trees, and Lactobacillus was isolated from honey stomachs using selective media. The isolates were Gram-stained and tested for catalase reaction. The 16S rRNA genes from extracted DNA of bacterial colonies were amplified with polymerase chain reaction using lactobacilli genus primers (27F and 1492R). All bacterial 16S rRNA genes were sequenced and deposited in GenBank. The 34 isolated strains yielded three distinct rRNA sequences of 15 different strains. Lactobacillus sequences isolated from the bees' honey stomachs were comprised of Lactobacillus kunkeei related-sequences (56%) with other abundant sequences being related to other Lactobacillus sp. (38%) and Lactobacillus vermiform (6%). These strains can be good candidates for potential application as probiotics in honeybees and also as natural food preservatives, which, in turn, may be useful in the food industry.


Apis dorsata honey stomach Lactobacillus bacteria probiotics 



This study had funding support from the Food Biotechnology Research Laboratory (Professor Yazid Laboratory), Faculty of Food Sciences and Technology, Universiti Putra Malaysia. The authors would like to thank Mr. A. Javanmard, Mr. M.M. Saberioon (Department of Agriculture Technology), Dr. Andony Melathopoulos (Agriculture and Agri-food of Canada) and all the honey hunters in Kedah state of Malaysia for providing honeybee samples.

Détection et identification de bactéries Lactobacillus du jabot de l’abeille géante Apis dorsata

Apis dorsata / jabot / Lactobacillus / probiotiques

Nachweis und Identifizierung von Lactobacillus Bakterien im Honigmagen der Riesenhonigbiene Apis dorsata

Apis dorsata / honigmagen / Lactobacillus Bakterien / probiotische Lebensmittel


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Copyright information

© INRA, DIB-AGIB and Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Naser Tajabadi
    • 1
    • 4
  • Makhdzir Mardan
    • 1
  • Mohd Yazid Abdul Manap
    • 2
  • Mustafa Shuhaimi
    • 3
  • Amir Meimandipour
    • 5
  • Leila Nateghi
    • 6
  1. 1.Department of Agriculture Technology, Faculty of AgricultureUniversiti Putra MalaysiaSerdangMalaysia
  2. 2.Department of Food Technology, Faculty of Food Sciences and TechnologyUniversiti Putra MalaysiaSerdangMalaysia
  3. 3.Department of Microbiology, Faculty of Biotechnology and Biomolecular SciencesUniversiti Putra MalaysiaSerdangMalaysia
  4. 4.Department of Honey BeeAnimal Science Research Institute of IranKarajIran
  5. 5.Department of Animal and Marine BiotechnologyNational Institute of Genetic Eengineering and Biotechnology (NIGEB)TehranIran
  6. 6.Department of Food Science and TechnologyVaramin-Pishva Branch, Islamic Azad UniversityVaraminIran

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