Detection and identification of Lactobacillus bacteria found in the honey stomach of the giant honeybee Apis dorsata
- 422 Downloads
This is the first assay that describes the isolation and identification of strains and species of Lactobacillus from the honey stomach of the Asiatic giant honeybee, Apis dorsata. Samples of honeybees were collected from A. dorsata colonies in different bee trees, and Lactobacillus was isolated from honey stomachs using selective media. The isolates were Gram-stained and tested for catalase reaction. The 16S rRNA genes from extracted DNA of bacterial colonies were amplified with polymerase chain reaction using lactobacilli genus primers (27F and 1492R). All bacterial 16S rRNA genes were sequenced and deposited in GenBank. The 34 isolated strains yielded three distinct rRNA sequences of 15 different strains. Lactobacillus sequences isolated from the bees' honey stomachs were comprised of Lactobacillus kunkeei related-sequences (56%) with other abundant sequences being related to other Lactobacillus sp. (38%) and Lactobacillus vermiform (6%). These strains can be good candidates for potential application as probiotics in honeybees and also as natural food preservatives, which, in turn, may be useful in the food industry.
KeywordApis dorsata honey stomach Lactobacillus bacteria probiotics
This study had funding support from the Food Biotechnology Research Laboratory (Professor Yazid Laboratory), Faculty of Food Sciences and Technology, Universiti Putra Malaysia. The authors would like to thank Mr. A. Javanmard, Mr. M.M. Saberioon (Department of Agriculture Technology), Dr. Andony Melathopoulos (Agriculture and Agri-food of Canada) and all the honey hunters in Kedah state of Malaysia for providing honeybee samples.
Détection et identification de bactéries Lactobacillus du jabot de l’abeille géante Apis dorsata
Apis dorsata / jabot / Lactobacillus / probiotiques
Nachweis und Identifizierung von Lactobacillus Bakterien im Honigmagen der Riesenhonigbiene Apis dorsata
Apis dorsata / honigmagen / Lactobacillus Bakterien / probiotische Lebensmittel
- Huang, Y.C., Edwards, C.G., Peterson, J.C. (1996) Relationship between sluggish fermentations and the antagonism of yeast by lactic acid bacteria. Am. J. Enol. Vitic. 47, 1–10Google Scholar
- Lane, D.J. (1991) 16S/23S rRNA sequencing. In: Stackebrandt, E., Goodfellow, M. (eds.) Nucleic acid techniques in bacterial systematics, pp. 115–175. Wiley, NewYorkGoogle Scholar
- Lee, C.Y., Kime, R.W. (1984) The use of honey for clarifying apple juice. J. Apic. Res. 23, 45–49Google Scholar
- Mardan, M., Kiew, R. (1985) Flowering periods of plants visited by honeybees in two areas of Malaysia, Proc. 3 Int. Conf Apic, Trop, Climates, Nairobi, 209–216.Google Scholar
- Shuhaimi, M. (2003) Species classification and molecular studies of bile salt hydrolase gene in Bifidobacterium spp, Doctor of philosophy, University Putra Malaysia, 1–174Google Scholar
- Sokal, R., Rohlf, F. (1995) Biometry. WH Freeman and Company, New YorkGoogle Scholar
- Steinkraus K.H. (1995) Handbook of indigenous fermented foods, Dekker New York NY.Google Scholar