Properties of self-sterile but cross-fertile allopolyploids synthesized between Brassica rapa and Raphanus sativus
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To expand the availability of intergeneric allopolyploids between Brassica rapa and Raphanus sativus beyond BB#1, a stable xBrassicoraphanus koranhort (baemoochae) cultivar (2n = 38, aAARR genome), we developed four intergeneric hybrids between different materials from B. rapa and R. sativus. These hybrids produced neither self-pollinated seeds nor embryos in microspore culture. The hybrids, however, did produce seeds (F1F1) via cross-pollination with existing baemoochae and mooyangchae, another xBrassicoraphanus line (2n = 36, rCCRR genome) generated from a cross between R. sativus and B. oleracea at Huazhong Agriculture University, China. The hybrid plants (F1F1) produced more seeds than the previous generation in both self- and cross-pollination and embryos, even in the presence of an induced microspore mutation. The self-sterile, cross-fertile properties of the newly synthesized allopolyploids suggest possible mechanisms for fertility restoration and provide valuable information for the development of intergeneric baemoochae cultivars.
KeywordsIntergeneric hybrid xBrassicoraphanus koranhort Baemoochae Mooyangchae Synthetic line
This work was supported by the Institute of Planning and Evaluation for Technology, Ministry of Food, Agriculture, Forestry, and Fisheries of Korea (117045-3). We would also like to thank the personnel at BBI for their assistance in this work.
S–S L conducted all procedures of this study. C Y S worked on the microspore culture of xBrassicoraphanus koranhort. J K proposed ideas and worked on medium development. J E P worked on pollination and made CAPS markers. S H Y made and tested CAPS. G Y led work on the CAPS markers and prepared the manuscript. J H H instructed students as a professor.
Compliance with ethical standards
Conflict of interest
The authors declare no competing interests or conflicts of interest.
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