Horticulture, Environment, and Biotechnology

, Volume 60, Issue 5, pp 647–657 | Cite as

Root pruning increased bioactive compounds of hydroponically-grown Agastache rugosa in a greenhouse

  • Vu Phong Lam
  • Sung Jin Kim
  • Hyun Joo Lee
  • Jong Seok ParkEmail author
Research Report


The aim of this study was to determine the proper root pruning time and ratio for enhancing bioactive compound formation in Agastache rugosa without plant growth inhibition. The control (without root pruning) and five treatments (1, 3, 5, 7, and 9 days of root pruning before harvesting (RPBH)) with 50% root pruning (Experiment 1) and four treatments of root pruning ratios with 30, 50, 70, and 90% root length at 5 days RBPH (Experiment 2) were performed in a hydroponic culture system. The results showed that shoot fresh and dry weights did not differ significantly between the 1, 3, 7, and 9 days RPBH and the control. There were no significant differences in shoot fresh and dry weights between 30 and 50% root pruning ratios and the control. The soil–plant analysis development (SPAD) chlorophyll meter was significantly decreased under ratios of 70 and 90% and 1 and 3 days RPBH, compared to other treatments. The rosmarinic acid (RA) and tilianin concentrations of A. rugosa under 9 days RPBH with 50% root pruning were significantly (105% and 141%) higher than those of the control. The acacetin concentration under 7 days RPBH with 50% root pruning was significantly (316%) higher than that of the control, while the RA and acacetin concentrations under 30% root pruning at 5 days were significantly (108% and 251%) higher than that of the control. These results indicated that 50% root pruning at 7 or 9 days before harvesting increased the concentrations of acacetin, RA, and tilianin and 30% root pruning at 5 days before harvesting increased in the levels of acacetin and RA concentration in A. rugosa without plant growth inhibition.


Acacetin Hoagland solution Plant growth Rosmarinic acid Tilianin 



This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through the Advanced Production Technology Development Program funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (317005–04-3).

Author Contributions

JSP Constructing idea, experimental design, data analysis, writing- original manuscript, writing- review and editing; VPL Setting up experiments, data collection and analysis, writing- original manuscript, writing- review and editing; SJK Preparation for the manuscript; HJL Rosmarinic acid, tilianin, and acacetin concentration analysis.

Compliance with Ethical Standards

Informed consent

All study participants provided informed consent.

Conflict of interest

There are no conflicts of interest to declare.


  1. Azquez DAJ, Hernandez GBM, Rodríguez SDC, Cao CM, Zevallos LC (2011) Plants as biofactories: physiological role of reactive oxygen species on the accumulation of phenolic antioxidants in carrot tissue under wounding and hyperoxia stress. J Agric Food Chem 59:6583–6593. CrossRefGoogle Scholar
  2. Bar-Tal A, Feigin A, Sheinfeld S, Rosenberg R, Sternbaum B, Rylski I, Pressman E (1995) Root restriction and N-NO3 solution concentration effects on nutrient-uptake, transpiration and dry-matter production of tomato. Sci Hortic 63:195–208. CrossRefGoogle Scholar
  3. Budiarto R, Poerwanto R, Santosa E, Efendi D (2019) A review of root pruning to regulate citrus growth. J Trop Crop 6:1–7Google Scholar
  4. Caliskan O, Radusiene J, Temizel KE, Staunis Z, Cirak C, Kurt D, Odabas MS (2017) The effects of salt and drought stress on phenolic accumulation in greenhouse-grown Hypericum pruinatum. Ital J Agron 12:271–275. CrossRefGoogle Scholar
  5. Carmi A (1995) Growth, water transport and transpiration in root-restricted plants of bean, and their relation to abscisic-acid accumulation. Plant Sci 107:69–76. CrossRefGoogle Scholar
  6. Du ZY, Xing SJ, Ma BY, Liu FC, Ma HL, Wang QH (2012) Effects of root pruning on the growth and rhizosphere soil characteristics of short-rotation closed-canopy poplar. Forest Syst 21:236–246. CrossRefGoogle Scholar
  7. Dubik SP, Krizek DT, Stimart DP (1990) Influence of root zone restriction on mineral element concentration, water potential, chlorophyll concentration, and partitioning of assimilate in spreading euonymus (Euonymus-Kiautschovica Loes Sieboldiana). J Plant Nutr 13:677–699. CrossRefGoogle Scholar
  8. Ekor M (2014) The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol 4:177. CrossRefPubMedPubMedCentralGoogle Scholar
  9. Esfandiari E, Shakiba MR, Mahboob SA, Alyari H, Shahabivand S (2008) The effect of water stress on the antioxidant content, protective enzyme activities, proline content and lipid peroxidation in wheat seedling. Pak J Biol Sci 11:1916–1922. CrossRefPubMedGoogle Scholar
  10. Jeong KJ, Park CM, Kang JS, Choi KO, Yun JG (2017) Rooting response of foliage plants affected by rooting stimulator, shading, and root pruning during transplanting to hydroball for hydroculture. Hortic Sci Technol 35:667–679Google Scholar
  11. Jing DW, Liu FC, Wang MY, Ma HL, Du ZY, Ma BY, Dong YF (2017) Effects of root pruning on the physicochemical properties and microbial activities of poplar rhizosphere soil. PLoS ONE 12(11):e0187685. CrossRefPubMedPubMedCentralGoogle Scholar
  12. Jing DW, Du ZY, Wang MY, Wang QH, Ma HL, Liu FC, Dong YF (2018) Regulatory effects of root pruning on leaf nutrients, photosynthesis, and growth of trees in a closed-canopy poplar plantation. PLoS ONE 13(5):e0197515. CrossRefPubMedPubMedCentralGoogle Scholar
  13. Kharkina TG, Ottosen CO, Rosenqvist E (1999) Effects of root restriction on the growth and physiology of cucumber plants. Physiol Plant 105:434–441. CrossRefGoogle Scholar
  14. Kim SJ, Bok KJ, Lam VP, Park JS (2017) Response of nutrient solution and photosynthetic photon flux density for growth and accumulation of antioxidant in Agastache rugosa under hydroponic culture systems. Protected Hortic Plant Factory 26:249–257. CrossRefGoogle Scholar
  15. Kim SJ, Park JE, Bok GJ, Kanth BK, Lam VP, Park JS (2018) High electrical conductivity of nutrient solution and application of methyl jasmonate promote phenylpropanoid production in hydroponically grown Agastache rugosa. Hortic Sci Technol 36:841–852. CrossRefGoogle Scholar
  16. Kudoyarova GR, Dodd IC, Veselov DS, Rothwell SA, Veselov SY (2015) Common and specific responses to availability of mineral nutrients and water. J Exp Bot 66:2133–2144. CrossRefPubMedPubMedCentralGoogle Scholar
  17. Li Q, Krauss MR, Hempfling WP (2006) Wounding of root or basal stalk prior to harvest affects pre-harvest antioxidant accumulation and tobacco-specific nitrosamine formation during air curing of burley tobacco (Nicotiana tabacum L.). J Agron Crop Sci 192:267–277. CrossRefGoogle Scholar
  18. Ma SC, Li FM, Yang SJ, Li CX, Xu BC, Zhang XC (2013) Effects of root pruning on non-hydraulic root-sourced signal, drought tolerance and water use efficiency of winter wheat. J Integr Agric 12:989–998. CrossRefGoogle Scholar
  19. MacKay BR (1995) Root restriction and root-shoot relationships in tomato (Lycopersicon esculentum Mill.). Doctoral dissertation. doi:10179/3004Google Scholar
  20. Mugnai HA, Da S (2011) Starch accumulation in the leaves of root-restricted pepper affects plant growth by a feedback-inhibition of the photosynthesis. Adv Hortic Sci 25:253–259Google Scholar
  21. Murshed R, Lopez-Lauri F, Sallanon H (2013) Effect of water stress on antioxidant systems and oxidative parameters in fruits of tomato (Solanum lycopersicon L, cv. Micro-tom). Physiol Mol Biol Plants 19:363–378. CrossRefPubMedPubMedCentralGoogle Scholar
  22. Nishizawa T, Saito K (1998) Effects of rooting volume restriction on the growth and carbohydrate concentration in tomato plants. J Am Soc Hortic Sci 123:581–585CrossRefGoogle Scholar
  23. Reyes L, Cisneros-Zevallos L (2003) Wounding stress increases the phenolic content and antioxidant capacity of purple-flesh potatoes (Solanum tuberosum L.). J Agric Food Chem 51:5296–5300. CrossRefPubMedGoogle Scholar
  24. Reyes LF, Villarreal JE, Cisneros-Zevallos L (2007) The increase in antioxidant capacity after wounding depends on the type of fruit or vegetable tissue. Food Chem 101:1254–1262. CrossRefGoogle Scholar
  25. Robbins NS, Pharr DM (1988) Effect of restricted root-growth on carbohydrate-metabolism and whole plant-growth of Cucumis-Sativus L. Plant Physiol 87:409–413. CrossRefPubMedPubMedCentralGoogle Scholar
  26. Saeedfar S, Negahban M, Soorestani MM (2015) The effect of drought stress on the essential oil content and some of the biochemical characteristics of anise hyssop (Agastache foeniculum [Pursh] Kuntze). Eur J Mol Biotechnol 8:103–114. CrossRefGoogle Scholar
  27. Salachas G, Savvas D, Argyropoulou K, Tarantillis PA, Kapotis G (2015) Yield and nutritional quality of aeroponically cultivated basil as affected by the available root-zone volume. Emir J Food Agric 27:911–918. CrossRefGoogle Scholar
  28. Sato F, Yoshioka H, Fujiwara, T, (2001) Effect of root volume restriction on growth and carbohydrate status of cabbage (Brassica oleracea capitata) seedlings. Environ Control Biol 39:235–243. CrossRefGoogle Scholar
  29. Seo TC, Rhee HC, Yun HK, Chun C (2010) Effect of root zone restriction on the growth response and phytonutrients contents of leafy vegetables grown in a DFT system. Korean J Hortic Sci 28:415–422Google Scholar
  30. Shi K, Ding XT, Dong DK, Zhou YH, Yu JQ (2008) Root restriction-induced limitation to photosynthesis in tomato (Lycopersicon esculentum Mill.) leaves. Sci Hortic 117:197–202. CrossRefGoogle Scholar
  31. Shin S (2004) Essential oil compounds from Agastache rugosa as antifungal agents against Trichophyton species. Arch Pharm Res 27:295–299. CrossRefPubMedGoogle Scholar
  32. Sun C, Li X, Hu Y, Zhao P, Xu T, Sun J, Gao X (2015) Proline, sugars, and antioxidant enzymes respond to drought stress in the leaves of strawberry plants. Hortic Sci Technol 33:625–632. CrossRefGoogle Scholar
  33. Tuan P, Park W, Xu H, Park N, Park S (2012) Accumulation of tilianin and rosmarinic acid and expression of phenylpropanoid biosynthetic genes in Agastache rugosa. J Agric Food Chem 60:5945–5951. CrossRefPubMedGoogle Scholar
  34. Vaniersel M (1997) Root restriction effects on growth and development of salvia (Salvia splendens). HortScience 32:1186–1190CrossRefGoogle Scholar
  35. Vysotskaya LB, Cherkoz'yanova AV, Veselov SY, Kudoyarova GR (2007) Role of auxins and cytokinins in the development of lateral roots in wheat plants with several roots removed. Russ J Plant Physiol 54:402–406. CrossRefGoogle Scholar
  36. Wang KC, Chang JS, Chiang LC, Lin CC (2009) 4-Methoxycinnamaldehyde inhibited human respiratory syncytial virus in a human larynx carcinoma cell line. Phytomedicine 16:882–886. CrossRefPubMedGoogle Scholar
  37. Wang YF, Bertelsen MG, Petersen KK, Andersen MN, Liu F (2014) Effect of root pruning and irrigation regimes on leaf water relations and xylem ABA and ionic concentrations in pear trees. Agric Water Manag 135:84–89. CrossRefGoogle Scholar
  38. Yadav SR, Helariutta Y (2014) Programmed cell death: new role in trimming the root tips. Curr Biol 24:374–376. CrossRefGoogle Scholar
  39. Yang S, Xing S, Liu C, Du Z, Wang H, Xu Y (2010) Effects of root pruning on the vegetative growth and fruit quality of Zhanhuadongzao trees. Hortic Sci 37:14–21. CrossRefGoogle Scholar
  40. Zielinska S, Matkowski A (2014) Phytochemistry and bioactivity of aromatic and medicinal plants from the genus Agastache (Lamiaceae). Phytochem Rev 13:391–416. CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Korean Society for Horticultural Science 2019

Authors and Affiliations

  1. 1.Department of Horticultural ScienceChungnam National UniversityDaejeonKorea

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