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A new interspecific hybrid of Torenia obtained through ovule culture

  • Tanapoom LaojuntaEmail author
  • Takako Narumi-Kawasaki
  • Takejiro Takamura
  • Seiichi Fukai
Research Report
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Abstract

Torenia is one of the most popular summer bedding plants in Japan. Commercially available hybrids are assumed to be interspecific hybrids derived from T. fournieri, T. concolor, and T. baillonii. Use of new Torenia species is required to produce novel flower types of interspecific hybrids. Three species from Thailand, T. bicolor, T. hirsutissima, and T. siamensis, were selected and crossed with T. fournieri, T. concolor, and T. baillonii. The chromosome number of six Torenia species varied from 2n = 16 to 2n = 34. Mature seeds were obtained from only 3 of 30 reciprocal cross combinations: T. fournieri × T. bicolor, T. fournieri × T. baillonii, and T. bicolor × T. baillonii. Ovule culture was applied to overcome the interspecific crossing barrier of Torenia, ovule culture was applied. Swollen ovules (250) were taken from pods at 10 days after crossing and cultured on MS medium. Progenies were obtained in 14 cross combinations, through ovule culture. This is the first interspecific Torenia hybrid obtained using T. bicolor, T. hirsutissima, and T. siamensis. The hybridity of all the obtained plants was confirmed using PCR–RFLP analysis of rDNA. The obtained hybrids showed intermediate morphological characteristics and pigmentation of floral organs between parents. This study shows that ovule culture is a powerful tool for overcoming the crossing barrier in interspecific crosses in Torenia.

Keywords

Pollen germination Sterile pollen Chromosome number Mature seed PCR–RFLP Pigments 

Notes

Acknowledgements

We thank Dr. Piyakaset Suksatan, Director of Garden and Development Department of the Queen Sirikit Botanical Garden for his valuable comments and support for the Torenia species.

Funding

This study was supported by the (JSPS KAKENHI) under Grant Number (JP17K017640).

References

  1. Abbas SR, Gardazi SDA, Sabir SM, Shah AH, Abbas MR, Batool A (2013) “ABBAS” DNA extraction method from plant. Int J Sci Eng Res 4:989–994Google Scholar
  2. Aida R, Shibata M (1995) Agrobacterium-mediated transformation of torenia (Torenia fournieri). Breed Sci 45:71–74.  https://doi.org/10.1270/jsbbs1951.45.71 Google Scholar
  3. Asano Y (1984) Fertility of a hybrid between distantly related species in Lilium. Cytologia 49:447–456.  https://doi.org/10.1508/cytologia.49.447 CrossRefGoogle Scholar
  4. Compton ME (1994) Statistical methods suitable for the analysis of plant tissue culture data. Plant Cell Tissue Org 37:217–242.  https://doi.org/10.1007/BF00042336 Google Scholar
  5. De Jeu MJ, Jacobsen E (1995) Early postfertilization ovule culture in Alstroemeral L. and barriers to interspecific hybridization. Euphytica 86:15–23.  https://doi.org/10.1007/BF00035934 CrossRefGoogle Scholar
  6. Haruki K, Hosoki T, Nako Y, Ohta K (1997) Possibility of classification in some species of Lilium by PCR-PFLP of ribulose-1,5-biphosphate carboxylase large subunit (rbcL) gene and ribosomal RNA gene. J Jpn Soc Hortic Sci 66:189–192.  https://doi.org/10.2503/jjshs.66.189 CrossRefGoogle Scholar
  7. Hsieh HM, Yang CK (2002) Revision of Torenia L. (Scophulariaceae) in Taiwan. Taiwania 47:281–289.  https://doi.org/10.6165/tai.2002.47(4).281 Google Scholar
  8. Jiranapapan J, Kikuchi S, Manachai B, Taychasinpitak T, Tanaka H, Tsujimoto H (2011) A simple method of chromosome doubling using colchicine in Torenia (Linderniaceae), and the behavior of meiotic chromosome in amphidiploids. Chromosome Sci 14:29–32.  https://doi.org/10.11352/scr.14.29 Google Scholar
  9. Kikuchi S, Kishii M, Shimizu M, Tsujimoto H (2005) Centromere-specific repetitive sequence from Torenia, a model plant for interspecific fertilization, and whole-mount FISH of its interspecific hybrid embryos. Cytogenet Genome Res 109:228–235.  https://doi.org/10.1159/000082405 CrossRefGoogle Scholar
  10. Kikuchi S, Kino H, Tanaka H, Tsujimoto H (2007) Pollen tube growth in cross combination between Torenia fournieri and fourteen related species. Breed Sci 57:117–122.  https://doi.org/10.1270/jsbbs.57.177 CrossRefGoogle Scholar
  11. Laojunta T, Narumi-Kawasaki T, Fukai S (2017) Characteristics of commercial Torenia cultivars. Acta Hortic 1167:205–211.  https://doi.org/10.17660/ActaHortic.2017.1167.31 CrossRefGoogle Scholar
  12. Lim KB, Ramanna MS, De Jong JH, Jacobsen E, Van Tuyl JM (2001) Indeterminate meiotic restitution (IMR): a novel type of meiotic nuclear restitution mechanism detected in interspecific lily hybrids by GISH. Theor Appl Genet 103:219–230.  https://doi.org/10.1007/s001220100638 CrossRefGoogle Scholar
  13. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plantarum 15:473–497.  https://doi.org/10.1111/j.1399-3054.1962.tb08052.x CrossRefGoogle Scholar
  14. Nontaswatsri C, Suksathan P (2014) Interspecific hybridization in the genus Globba using in vitro embryo culture. Acta Hortic 1025:31–36.  https://doi.org/10.17660/ActaHortic.2014.1025.3 CrossRefGoogle Scholar
  15. Nuntha B, Kikuchi S, Taychasinpitak T, Sassa H, Koba T (2016) New karyotypes of an interspecific hybrid of Torenia fournieri and Torenia baillonii and its progenies. Chromosome Sci 19:37–40.  https://doi.org/10.11352/scr.19.37 Google Scholar
  16. Pathirana R, Wiedow C, Pathirana S, Hedderley D, Morgan E, Scalzo J, Frew T, Timmerman-Vaughan G (2015) Ovule culture and embryo rescue facilitate in interspecific hybridization in blueberry (Vaccinium spp.). Acta Hortic.  https://doi.org/10.17660/actahortic.2015.1083.13 Google Scholar
  17. Ramanna MS (1979) A re-examination of the mechanisms of 2n gamete formation in potato and it implication for breeding. Euphytica 28:537–561.  https://doi.org/10.1007/BF00038921 CrossRefGoogle Scholar
  18. Rodrigo JM, Zappacosta DC, Selva JP, Garbus I, Albertini E, Echenique V (2017) Apomixis frequency under stress conditions in weeping lovegrass (Eragrostis curvula). PLoS ONE 12:e0175852.  https://doi.org/10.1371/journal.pone.0175852 CrossRefGoogle Scholar
  19. Sawangmee W, Taychasinpitak T, Jompuk P, Kikuchi S (2011) Effect of gamma-ray irradiation in plant morphology of interspecific hybrids between Torenia fournieri and Torenia baillonii. Kasetsart J Nat Sci 45:803–810Google Scholar
  20. Tomasi P, Dierig D, Dahlquist G (2002) An ovule culture technique for producing interspecific Lesquerella hybrids. In: Janick J, Whipkey A (eds) Trends in new crops and new uses. ASHS Press, Alexandria, VA, pp 208–212Google Scholar
  21. Van Tuyl JM, De Jeu MJ (1997) Methods for overcoming interspecific crossing barriers. Chapter 13. In: Sawhney, Shivanna (eds) Pollen biotechnology for crop production and improvement. Cambridge University Press, Cambridge, pp 273–293CrossRefGoogle Scholar
  22. Van Tuyl JM, Van Dien MP, Van Creij MGM, Van Kleinwee TCM, Franken J, Bino RJ (1991) Application of in vitro pollination, ovary culture, ovule culture and embryo rescue for overcoming incongruity barriers in interspecific Lilium crosses. Plant Sci 74:115–126.  https://doi.org/10.1016/0168-9452(91)90262-7 CrossRefGoogle Scholar
  23. Van Tuyl JM, Maas IWGM, Lim KB (2002) Introgression in interspecific hybrids of lily. Acta Hortic 570:213–218.  https://doi.org/10.17660/ActaHortic.2002.570.25 CrossRefGoogle Scholar
  24. Yamasaki T (1985) A revision of the genera Limnophila and Torenia from Indochina. J Fac Sci Univ Tokyo III 13:575–625Google Scholar

Copyright information

© Korean Society for Horticultural Science 2019

Authors and Affiliations

  • Tanapoom Laojunta
    • 1
    Email author
  • Takako Narumi-Kawasaki
    • 1
  • Takejiro Takamura
    • 1
  • Seiichi Fukai
    • 1
  1. 1.Horticultural Science, Faculty of AgricultureKagawa UniversityKagawaJapan

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