Propagation methods for gametophyte proliferation and sporophyte formation in silver cloak fern (Cheilanthes argentea)

  • Bo Kook Jang
  • Ju Sung Cho
  • Cheol Hee LeeEmail author
Research Report


Cheilanthes argentea (S.G. Gmel.) Kunze is a highly valued indigenous/endemic Korean bracken species used for ornamental purposes. In this study, we developed a practical proliferation method for mass production of C. argentea plants using tissue culture. The gametophyte proliferation using the chopping method produced gametophytes that were morphologically identical to those obtained from in vitro spore germination. This new method increased plant fresh weight by more than 40-fold, from 300 mg to 12.7 g, in Murashige and Skoog medium. A blending method was used to produce 74.3 sporophytes in 7.5-cm2 plastic pots using 1 g of gametophytes. Furthermore, addition of exogenous gibberellin promoted sporophyte development and growth, suggesting the possibility of controlling the number of sporophytes formed. However, further studies are needed to explain the effect of exogenous gibberellin, which is closely related to the sex-determining hormone antheridiogen, on the number of sporophytes and its mechanism of action. Our new tissue culture system is capable of mass proliferation of C. argentea gametophytes in vitro and the formation of sporophytes by ex vitro preparation using the generated gametophytes.


Cheilanthes argentea (S.G. Gmel.) Kunze gametophyte proliferation Gibberellin In vitro spore germination Sporophytes 



This study was financially supported by the project “Development of Mass propagation and Production Techniques on Useful Exploration Plant Resources” of the Korea National Arboretum, Project No. KNA1-2-15, 11-6. This study was also financially supported by the project “Development of Mass Propagation Protocol for the Establishment of Application Base Using Forest Plants” of the Korea National Arboretum, Project No. KNA1-2-25, 16-3.


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Copyright information

© Korean Society for Horticultural Science 2019

Authors and Affiliations

  1. 1.Division of Animal, Horticultural and Food SciencesChungbuk National UniversityCheongjuRepublic of Korea
  2. 2.Brain Korea 21 Center for Bio-Resource DevelopmentChungbuk National UniversityCheongjuRepublic of Korea

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