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Ginsenoside accumulation profiles in long- and short-term cell suspension and adventitious root cultures in Panax ginseng

  • Kim-Cuong Le
  • Cheol-Seung Jeong
  • Hyoshin Lee
  • Kee-Yoeup Paek
  • So-Young Park
Research Report
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Abstract

In this study, we compared two in vitro culture systems, dedifferentiated cell suspension culture and differentiated adventitious root culture, using inoculum from long-term (20-year culture period) and short-term (1-year culture period) cultures, for the production of ginsenosides in wild ginseng (Panax ginseng). Increases in biomass and ginsenoside content were monitored in a 3-L bioreactor. The biomass in short-term cell suspension and adventitious root cultures increased at a faster rate than that in the long-term cultures, reaching a peak after 4 and 8 weeks of culture, respectively. In long term cultured materials, the biomass of cell suspension and adventitious root cultures decreased by 1.67- and 1.52-fold, respectively. Although the amount of ginsenosides in cell suspension and adventitious root cultures varied during the culture periods, the total ginsenoside content remained constant. These data suggest that both culture systems are advantageous for the production of different ginsenosides for pharmaceutical purposes.

Keywords

Adventitious roots Biomass Bioreactor culture Cell suspension Ginsenoside Panax ginseng 

Notes

Acknowledgements

This study was funded by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through the Advanced Production Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (Grant Number 315013-4), and Kim-Cuong Le was supported by the Brain Korea 21 (BK21) Plus program.

Author’s contribution

K-C L contributed to data acquisition and the writing of the manuscript. C-S J, H L, and K-Y P participated in data interpretation and revising of the manuscript to include important intellectual content. S-Y P made substantial contributions to data interpretation, revising of the manuscript, the conception, and design of this study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

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Copyright information

© Korean Society for Horticultural Science 2018

Authors and Affiliations

  • Kim-Cuong Le
    • 1
  • Cheol-Seung Jeong
    • 1
    • 2
  • Hyoshin Lee
    • 3
  • Kee-Yoeup Paek
    • 1
    • 2
  • So-Young Park
    • 1
  1. 1.Division of Animal, Horticulture and Food SciencesChungbuk National UniversityCheongjuRepublic of Korea
  2. 2.WellGreen Co.CheongjuRepublic of Korea
  3. 3.Department of Forest Genetic ResourcesNational Institute of Forest ScienceSuwonRepublic of Korea

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