Enhanced accumulation of triterpenoid saponins in in vitro plantlets and dedifferentiated cultures of Eryngium planum L.: a medicinal plant
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We investigated the conditions leading to the accumulation of saponins in plants using in vitro cultures of Eryngium planum L. The aim was to establish a system that produced saponins of the same quality as those found in field-grown plants and to enhance their accumulation in the laboratory. We observed that the content of saponins in organs from in vitro plantlets and plants grown in an experimental plot after micropropagation was higher (up to 8.7 times) compared with plants from natural sites. Untreated callus accumulated saponins in amounts similar to those of the roots from field-grown plants. Applying sucrose to the growth medium at higher concentrations (40, 50, or 60 g L−1) resulted in enhanced saponin production in callus (1.2–1.7-fold). The elicitation with 100 µmol L−1 methyl jasmonate of callus grown on Murashige and Skoog medium supplemented with 30 g L−1 sucrose resulted in a 1.2-fold increased saponin accumulation compared with callus maintained on the same medium (30 g L−1 sucrose). This study provides the first evidence that triterpenoid saponins can be biosynthesized in in vitro plantlets and dedifferentiated cultures of E. planum.
KeywordsFlat sea holly In vitro cultures Methyl jasmonate Saponins Sucrose
This study was supported by the Ministry of Science and Higher Education, Warsaw, Poland, from educational sources: in 2008–2011 as Grant No. NN 405 065334, and in 2011–2013 as Grant No. NN 405 683340.
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