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The establishment of a DNA fingerprinting database for 73 varieties of Lactuca sativa capitate L. using SSR molecular markers

  • Huiyan Zhou
  • Penghang Zhang
  • Jiang Luo
  • Xueying Liu
  • Shuangxi Fan
  • Chaojie Liu
  • Yingyan Han
Research Report
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Abstract

Head lettuce originating near the Mediterranean coast was introduced to China in the fifth century. It is difficult to identify different varieties of head lettuce due to their close genetic relationships. Simple sequence repeats (SSR) technology is a practical method that has been widely used in variety identification, fingerprint construction and genetic diversity analysis. In this paper, 23 pairs of SSR primers were selected to identify and analyse 73 varieties of head lettuce. The results identified a total of 117 mutated alleles detected in 23 loci, with the number of each loci ranging from 2 to 11, with an average of 5.1 mutated alleles per locus. Additionally, 152 genotypes were detected in 23 loci, with an average of 6.6 genotypes per locus derived from a range of 2–13. The polymorphism information content ranged from 0.071 to 0.702, with an average of 0.499. The selected primer pairs could clearly reflect the genotypic diversity level of the 73 head lettuce varieties tested, and can be used in the detection and analysis of the head lettuce varieties. The results of our sequence analysis also showed that these 73 varieties of head lettuce could be effectively identified by a combination of 19 primer pairs even though the genetic similarity among the 73 varieties ranged from 0.647 to 0.991, with an average of 0.798. Based on a capillary electrophoresis platform, a DNA fingerprinting database of 73 head lettuce varieties was established.

Keywords

Head lettuce Lactuca sativa L. SSR Variety identification DNA fingerprinting database 

Notes

Acknowledgements

This work was supported by National Natural Science Foundation of China [31401883], the Beijing Innovation Consortium of Agriculture Research System (BAIC08), and the Beijing Municipal National Science Foundation (KZ201610020019).

Supplementary material

13580_2018_102_MOESM1_ESM.jpg (78 kb)
Trend analysis: the variety differentiation of primer pair combination (JPEG 78 kb)
13580_2018_102_MOESM2_ESM.pdf (74 kb)
DNA fingerprints of 73 lettuce varieties (PDF 74 kb)
13580_2018_102_MOESM3_ESM.pdf (102 kb)
Allelic loci of each primer pair (PDF 101 kb)

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Copyright information

© Korean Society for Horticultural Science 2018

Authors and Affiliations

  • Huiyan Zhou
    • 1
  • Penghang Zhang
    • 1
  • Jiang Luo
    • 1
  • Xueying Liu
    • 1
  • Shuangxi Fan
    • 1
  • Chaojie Liu
    • 1
  • Yingyan Han
    • 1
  1. 1.Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production EducationBeijing University of AgricultureBeijingChina

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