Advertisement

Horticulture, Environment, and Biotechnology

, Volume 59, Issue 5, pp 723–728 | Cite as

Low risk of pollen-mediated gene flow in transgenic plants under greenhouse conditions

  • Deuk-Su Kim
  • Ilchan Song
  • Kisung KoEmail author
Research Report Genetics and Breeding
  • 74 Downloads

Abstract

There is concern about the potential for transgene contamination in wild type (WT) plants via pollen-mediated gene flow from transgenic plants. In this study, we investigate pollen-mediated gene flow using tobacco transgenic lines carrying recombinant protein GA733-FcK, which is a putative candidate for producing colorectal cancer vaccine. Transgenic and WT plants were grown in greenhouse, with WT plants placed 0.3, 1, 5, 10, and 20 m away from transgenic plants. Seeds were harvested from randomly selected WT plants and sown on germination media supplemented with or without kanamycin. At 30 days after sowing, none of the WT seedlings produced true leaves and roots when grown on selective media. Polymerase chain reaction analysis revealed that GA733-FcK and nptII genes were expressed in shoots of transgenic plants but not of WT plants. Expression of GA733-FcK and nptII proteins was also abolished in WT leaves when compared to that of transgenic plants. Our findings suggested that there is low risk of pollen-mediated gene flow from transgenic plants expressing GA733-FcK when grown in greenhouse conditions.

Keywords

Pollen-mediated gene flow Transgenic Greenhouse Biosafety Agricultural biotechnology 

Notes

Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1A6A3A11930180), National Research Foundation of Korea Grant funded by the Korean Government (MEST) (NRF-2017R1A2A2A0569788), Korean Rural Development Administration (Code#PJ0134372018).

References

  1. Baltazar BM, Espinoza LC, Banda AE, Martinez JMD, Tiznado JAG, Garcia JG, Gutierrez MA, Rodriguez JLG, Diaz OH et al (2015) Pollen-mediated gene flow in maize: implications for isolation requirements and coexistence in Mexico, the center of origin of maize. PLoS ONE 10:e0131549CrossRefPubMedPubMedCentralGoogle Scholar
  2. Bohn T, Aheto DW, Mwangala FS, Fischer K, Bones IL, Simoloka C, Mbeule I, Schmidt G, Breckling B (2016) Pollen-mediated gene flow and seed exchange in small-scale Zambian maize farming, implications for biosafety assessment. Sci Rep 6:34483CrossRefPubMedPubMedCentralGoogle Scholar
  3. Chandler S, Dunwell JM (2008) Gene flow, risk assessment and the environmental release of transgenic plants. Crit Rev Plant Sci 27:25–49CrossRefGoogle Scholar
  4. Chen J, Ying GG, Wei XD, Liu YS, Liu SS, Hu LX, He LY, Chen ZF, Chen FR et al (2016) Removal of antibiotics and antibiotic resistance genes from domestic sewage by constructed wetlands: effect of flow configuration and plant species. Sci Total Environ 571:974–982CrossRefPubMedGoogle Scholar
  5. DiFazio SP, Leonardi S, Slavov GT, Garman SL, Adams WT, Strauss SH (2012) Gene flow and simulation of transgene dispersal from hybrid poplar plantations. New Phytol 193:903–915CrossRefPubMedGoogle Scholar
  6. Dong S, Liu Y, Yu C, Zhang Z, Chen M, Wang C (2016) Investigating pollen and gene flow of WYMV-resistant transgenic wheat N12-1 using a dwarf male-sterile line as the pollen receptor. PLoS ONE 11:e0151373CrossRefPubMedPubMedCentralGoogle Scholar
  7. Ellstrand NC (2003) Current knowledge of gene flow in plants: implications for transgene flow. Philos Trans R Soc Lond B Biol Sci 358:1163–1170CrossRefPubMedPubMedCentralGoogle Scholar
  8. Goggi AS, Lopez-Sanchez H, Caragea P, Westgate M, Arritt R, Clark CA (2007) Gene flow in maize fields with different local pollen densities. Int J Biometeorol 51:493–503CrossRefPubMedGoogle Scholar
  9. Heuberger S, Crowder DW, Brevault T, Tabashnik BE, Carriere Y (2011) Modeling the effects of plant-to-plant gene flow, larval behavior, and refuge size on pest resistance to Bt cotton. Environ Entomol 40:484–495CrossRefGoogle Scholar
  10. Husken A, Prescher S, Schiemann J (2010) Evaluating biological containment strategies for pollen-mediated gene flow. Environ Biosafety Res 9:67–73CrossRefPubMedGoogle Scholar
  11. Jamal A, Lee J-H, Lee K-J, Oh D-B, Kim D-S, Lee K-K, Choo Y-K, Hwang K-A, Ko K (2012) Chimerism of multiple monoclonal antibodies expressed in a single plant. Hortic Environ Biotechnol 53:544–551CrossRefGoogle Scholar
  12. Kang Y, Shin YK, Park S-W, Ko K (2016) Effect of nitrogen deficiency on recombinant protein production and dimerization and growth in transgenic plants. Hortic Environ Biotechnol 57:299–307CrossRefGoogle Scholar
  13. Kim KI, Yoo KH, Chung HY, Lee JH, Lee HH, Seok YJ, Hwang-Bo J, Cui EJ, Ko KS et al (2009) Expression of recombinant colorectal cancer antigen GA733-2 in plants and its immune response in mice. J Biosci Bioeng 108:S14–S15CrossRefGoogle Scholar
  14. Kim DS, Qiao L, Lee KJ, Ko K (2015) Optimization of colorectal cancer vaccine candidate protein GA733-Fc expression in a baculovirus-insect cell system. Entomol Res 45:39–48CrossRefGoogle Scholar
  15. Kwit C, Moon HS, Warwick SI, Stewart CN (2011) Transgene introgression in crop relatives: molecular evidence and mitigation strategies. Trends Biotechnol 29:284–293CrossRefPubMedGoogle Scholar
  16. Lee JH, Qiao L, Song MR, Ko K (2016) Murine response studies of insect cell (Sf9) expressed recombinant colorectal cancer vaccine candidate using surface plasmon resonance studies. Entomol Res 46:5–14CrossRefGoogle Scholar
  17. Lim CY, Lee KJ, Oh DB, Ko K (2015) Effect of the developmental stage and tissue position on the expression and glycosylation of recombinant glycoprotein GA733-FcK in transgenic plants. Front Plant Sci 5:778CrossRefPubMedPubMedCentralGoogle Scholar
  18. Loureiro I, Garcia-Ruiz E, Gutierrez E, Gomez P, Escorial MC, Chueca MC (2016) Pollen-mediated gene flow in the cultivation of transgenic cotton under experimental field conditions in Spain. J Indcrop 85:22–28Google Scholar
  19. Lu Z, Lee KJ, Shao Y, Lee JH, So Y, Choo YK, Oh DB, Hwang KA, Oh SH et al (2012) Expression of GA733-Fc fusion protein as a vaccine candidate for colorectal cancer in transgenic plants. J Biomed Biotechnol 2012:11Google Scholar
  20. Miroshnichenko D, Pushin A, Dolgov S (2016) Assessment of the pollen-mediated transgene flow from the plants of herbicide resistant wheat to conventional wheat (Triticum aestivum L.). Euphytica 209:71–84CrossRefGoogle Scholar
  21. Mosolits S, Harmenberg U, Rudén U, Ohman L, Nilsson B, Wahren B, Fagerberg J, Mellstedt H (1999) Autoantibodies against the tumour-associated antigen GA733-2 in patients with colorectal carcinoma. Cancer Immunol Immunother 47:315–320CrossRefPubMedGoogle Scholar
  22. Oh E-Y, Kim YK, Park D-Y, Lu Z, Choo YK, Han YS, Park JM, Ko K (2011) Expression of recombinant proteins in plants by using baculovirus vectors. Hortic Environ Biotechnol 52:95–104CrossRefGoogle Scholar
  23. Qaim M (2009) The economics of genetically modified crops. Ann Rev Resour Econ 1:665–693CrossRefGoogle Scholar
  24. Serrat X, Esteban R, Penas G, Catala MM, Mele E, Messeguer J (2013) Direct and reverse pollen-mediated gene flow between GM rice and red rice weed. Aob Plants.  https://doi.org/10.1093/aobpla/plt050 CrossRefPubMedCentralGoogle Scholar
  25. Snow AA (2002) Transgenic crops—why gene flow matters. Nat Biotechnol 20:542CrossRefPubMedGoogle Scholar
  26. Song I, Kim DS, Kim MK, Jamal A, Hwang K-A, Ko K (2015) Comparison of total soluble protein in various horticultural crops and evaluation of its quantification methods. Hortic Environ Biotechnol 56:123–129CrossRefGoogle Scholar

Copyright information

© Korean Society for Horticultural Science and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Medicine, College of MedicineChung-Ang UniversitySeoulKorea

Personalised recommendations