Pre-harvest ethylene control affects vase life of cut rose ‘Carola’ by regulating energy metabolism and antioxidant enzyme activity Research Report First Online: 31 October 2018 Abstract
We studied the role of ethylene control in regulating energy metabolism, antioxidant enzyme activity, and vase life of cut rose
Rosa hybrida ‘Carola’. Rose flowers at stage II were sprayed with one of the following solutions: water (control), 10 μL L −1 1-methylcyclopropene (1-MCP), or 0.5 g L −1 2-chloroethanephosphonic acid (ethephon). After harvest, ethylene production rate, respiration intensity, energy charge (EC), activities of energy metabolism-related and antioxidant enzymes, and malondialdehyde (MDA) content were measured. Results showed that 1-MCP enhanced the activities of superoxide dismutase, H +-adenosine triphosphatase, Ca 2+-adenosine triphosphatase, succinic dehydrogenase, and cytochrome c oxidase, increased adenosine triphosphate (ATP) content, maintained high EC levels, inhibited respiration intensity, reduced peroxidase (POD) and polyphenol oxidase (PPO) activity and MDA accumulation, and prolonged vase life. Ethephon promoted ethylene production and respiration intensity, increased POD and PPO activity, reduced ATP content and EC levels, and accelerated senescence. Our results support a novel role for ethylene control in regulating senescence of ‘Carola’. Keywords Energy status Ethephon Longevity Superoxide dismutase 1-methylcyclopropene
Bi Gong and Shuai Huang have contributed equally to this study.
We acknowledge Mr. Jin Wang, Department of Bio-resource Engineering, McGill University, Canada, for his useful modifications on language in this manuscript.
Alaey M, Babalar M, Naderi R, Kafi M (2011) Effect of pre- and postharvest salicylic acid treatment on physio-chemical attributes in relation to vase-life of rose cut flowers. Postharvest Biol Technol 61:91–94.
https://doi.org/10.1016/j.postharvbio.2011.02.002 CrossRef Google Scholar
Arora A, Sairam RK, Srivastava GC (2002) Oxidative stress and antioxidative system in plants. Curr Sci 82:1227–1238
Azad AK, Ishikawa T, Sawa Y, Shibata H (2008) Intracellular energy depletion triggers programmed cell death during petal senescence in tulip. J Exp Bot 59:2085–2095.
https://doi.org/10.1093/jxb/ern066 CrossRef PubMed PubMedCentral Google Scholar
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254.
https://doi.org/10.1016/0003-2697(76)90527-3 CrossRef Google Scholar
Cheng SC, Wei BD, Zhou Q, Tan DH, Ji SJ (2015) 1-Methylcyclopropene alleviates chilling injury by regulating energy metabolism and fatty acid content in ‘Nanguo’ pears. Postharvest Biol Technol 109:130–136.
https://doi.org/10.1016/j.postharvbio.2015.05.012 CrossRef Google Scholar
Duan XW, Jiang YM, Su XG, Liu H, Li YB, Zhang ZQ, Zheng YH, Jiang WB (2004) Role of pure oxygen treatment in browning of litchi fruit after harvest. Plant Sci 167:665–668.
https://doi.org/10.1016/j.plantsci.2004.05.009 CrossRef Google Scholar
Giannopolites CN, Ries SK (1977) Superoxide dismutase, I: occurrence in higher plants. Plant Physiol 59:309–314.
https://doi.org/10.1104/pp.59.2.309 CrossRef Google Scholar
Halevy AH (1986) Flower senescence. In: Leshem YY, Halevy AH, Frenkel C (eds) Processes and control of plant senescence. Elsevier, Amsterdam, pp 150–200
Harwood JL (1998) Fatty acid metabolism. Ann Rev Plant Physiol Mol Biol 39:101–138
CrossRef Google Scholar
Huang S, Gong B, Wei FJ, Ma HL (2017) Pre-harvest 1-methylcyclopropene application affects post-harvest physiology and storage life of the cut rose cv. carola. Hortic Environ Biotechnol 58:144–151.
https://doi.org/10.1007/s13580-017-0081-9 CrossRef Google Scholar
Jiang YM, Jiang YL, Qu HX, Duan XW, Luo YB, Jiang WB (2007) Energy aspects in ripening and senescence of harvested horticultural crops. Stewart Postharvest Rev 3:1–5.
https://doi.org/10.2212/spr.2007.2.5 CrossRef Google Scholar
Jin P, Zhu H, Wang J, Chen JJ, Wang XL, Zheng YH (2013) Effect of methyl jasmonate on energy metabolism in peach fruit during chilling stress. J Sci Food Agric 93:1827–1832.
https://doi.org/10.1002/jsfa.5973 CrossRef PubMed Google Scholar
Jin P, Zhu H, Wang L, Shan TM, Zheng YH (2014) Oxalic acid alleviates chilling injury in peach fruit by regulating energy metabolism and fatty acid contents. Food Chem 161:87–93.
https://doi.org/10.1016/j.foodchem.2014.03.103 CrossRef PubMed Google Scholar
Kebrom TH, Mckinley B, Mullet JE (2017) Dynamics of gene expression during development and expansion of vegetative stem internodes of bioenergy sorghum. Biotechnol Biofuels 10:159.
https://doi.org/10.1186/s13068-017-0848-3 CrossRef PubMed PubMedCentral Google Scholar
Kibinza S, Vinel D, Côme D, Bailly C, Corbineau F (2006) Sunflower seed deterioration as related to moisture content during ageing, energy metabolism and active oxygen species scavenging. Physiol Plant 128:496–506.
https://doi.org/10.1111/j.1399-3054.2006.00771.x CrossRef Google Scholar
Li D, Cheng Y, Dong Y, Shang Z, Guan J (2017) Effects of low temperature conditioning on fruit quality and peel browning spot in ‘huangguan’ pears during cold storage. Postharvest Biol Technol 131:68–73.
https://doi.org/10.1016/j.postharvbio.2017.05.005 CrossRef Google Scholar
Lin D, Zhao YY (2007) Innovations in the development and application of edible coatings for fresh and minimally processed fruits and vegetables. Compr Rev Food Sci Food Saf 6:60–75.
https://doi.org/10.1111/j.1541-4337.2007.00018.x CrossRef Google Scholar
Liu SH, Zang XP, Sun GM (2013) Changes in endogenous hormone concentrations during inflorescence induction and development in pineapple (
cv. Smooth Cayenne) by ethephon. Afr J Biotechnol 10:10892–10899.
https://doi.org/10.5897/AJB11.124 CrossRef Google Scholar
Lou BG, Wang AY, Lin C, Xu T, Zheng XD (2011) Enhancement of defense responses by oligandrin against botrytis cinerea in tomatoes. Afr J Biotechnol 10:11442–11449.
https://doi.org/10.5897/AJB11.618 CrossRef Google Scholar
Ma N, Cai L, Lu WJ, Tan H, Gao J (2005) Exogenous ethylene influences flower opening of cut roses (
) by regulating the genes encoding ethylene biosynthesis enzymes. Sci China Ser C 48:434–444.
https://doi.org/10.1360/062004-37 CrossRef Google Scholar
Mayak S, Halevy AH (1980) Flower senescence. In: Thimann K (ed) Senescence in plants. CRC Press, Boca Raton, pp 131–156
Meng XC, Yang GH, Sun H, Yu DM, Wang XJ, Wang P, Niu YM (2012) Effects of H
, paraquat, and ethephon on herbal drug quality of
based on reactive oxygen species system. Pharmacogn Mag 8:54–59.
https://doi.org/10.4103/0973-1296.93325 CrossRef Google Scholar
Meng Y, Li N, Tian J, Gao J, Zhang C (2013) Identification and validation of reference genes for gene expression studies in postharvest rose flower (
). Sci Hortic 158:16–21.
https://doi.org/10.1016/j.scienta.2013.04.019 CrossRef Google Scholar
Nergi MAD, Ahmadi N (2014) Effects of 1-MCP and ethylene on postharvest quality and expression of senescence-associated genes in cut rose cv. Sparkle. Sci Hortic 166:78–83.
https://doi.org/10.1016/j.scienta.2013.12.015 CrossRef Google Scholar
Rubinstein B (2000) Regulation of cell death in flower petals. Plant Mol Biol 44:303–318.
https://doi.org/10.1023/A:1026540524990 CrossRef PubMed Google Scholar
Saquet AA, Streif J, Bangerth F (2000) Changes in ATP, ADP and pyridine nucleotide levels related to the incidence of physiological disorders in ‘Conference’ pears and ‘Jonagold’ apples during controlled atmosphere storage. J Hortic Sci Biotech 75:243–249.
https://doi.org/10.1080/14620316.2000.11511231 CrossRef Google Scholar
Scariot V, Paradiso R, Rogers H, De Pascale S (2014) Ethylene control in cut flowers: classical and innovative approaches. Postharvest Biol Technol 97:83–92.
https://doi.org/10.1016/j.postharvbio.2014.06.010 CrossRef Google Scholar
Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative CT method. Nat Protoc 36:1101–1108.
https://doi.org/10.1038/nprot.2008.73 CrossRef Google Scholar
Serek M (1993) Ethephon and silver thiosulfate affect postharvest characteristics of
‘Victory Parade’. Hortscience 28:279–282
Shimizu-Yumoto H, Ichimura K (2013) Postharvest characteristics of cut dahlia flowers with a focus on ethylene and effectiveness of 6-benzylaminopurine treatments in extending vase life. Postharvest Biol Technol 86:479–486.
https://doi.org/10.1016/j.postharvbio.2013.07.036 CrossRef Google Scholar
Siboza XI, Bertling I, Odindo AO (2014) Salicylic acid and methyl jasmonate improve chilling tolerance in cold-stored lemon fruit (
). J Plant Physiol 171:1722–1731.
https://doi.org/10.1016/j.jplph.2014.05.012 CrossRef PubMed Google Scholar
Singh SP, Singh Z, Swinny EE (2012) Climacteric level during fruit ripeninginfluences lipid peroxidation and enzymatic and non-enzymatic antioxidativesystems in Japanese plums (
Lindell). Postharvest Biol Technol 65:22–32.
https://doi.org/10.1016/j.postharvbio.2011.10.007 CrossRef Google Scholar
Song LL, Peng YH (2004) Effect of cold storage on sensitivity of cut lily to ethylene. J Hortic Sci Biotech 79:723–728.
https://doi.org/10.1080/14620316.2004.11511833 CrossRef Google Scholar
Song LL, Liu H, You YL, Sun J, Yi C, Li YB (2014) Quality deterioration of cut carnation flowers involves in antioxidant systems and energy status. Sci Hortic 170:45–52.
https://doi.org/10.1016/j.scienta.2014.02.035 CrossRef Google Scholar
Sun J, You XR, Li L, Peng HX, Su WQ, Li CB, He QG, Liao F (2011) Effects of a phospholipase D inhibitor on postharvest enzymatic browning and oxidative stress of litchi fruit. Postharvest Biol Technol 62:288–294.
https://doi.org/10.1016/j.postharvbio.2011.07.001 CrossRef Google Scholar
Tomás-Barberán FA, Espín JC (2001) Phenolic compounds and related enzymes asdeterminants of quality in horticultural crops. J Sci Food Agric 81:853–876
CrossRef Google Scholar
Trippi VS, Paulin A, Pradet A (1988) Effect of oxygen concentration on the senescence and energy-metabolism of cut carnation flowers. Physiol Plant 73:374–379.
https://doi.org/10.1111/j.1399-3054.1988.tb00613.x CrossRef Google Scholar
Veltman RH, Lenthéric I, Van der Plas LHW, Peppelenbos HW (2003) Internal browning in pear fruit (
L., cv. Conference) may be a result of a limited availability of energy and antioxidants. Postharvest Biol Technol 28:295–302.
https://doi.org/10.1016/S0925-5214(02)00198-9 CrossRef Google Scholar
Wang H, Qian Z, Ma S, Zhou Y, Patrick JW, Duan X (2013) Energy status of ripening and postharvest senescent fruit of litchi (
Litchi chinensis S
onn.). BMC Plant Biol.
https://doi.org/10.1186/1471-2229-13-55 CrossRef PubMed PubMedCentral Google Scholar
Wisutiamonkul A, Ampomah-Dwamena C, Allan AC, Ketsa S (2017) Carotenoid accumulation in durian (
) fruit is affected by ethylene via modulation of carotenoid pathway gene expression. Plant Physiol Biochem 115:308–319.
https://doi.org/10.1016/j.plaphy.2017.03.021 CrossRef PubMed Google Scholar
Wu F, Zhang C, Wang X, Guo J, Dong L (2017) Ethylene-influenced development of tree peony cut flowers and characterization of genes involved in ethylene biosynthesis and perception. Postharvest Biol Technol 125:150–160.
https://doi.org/10.1016/j.postharvbio.2016.11.014 CrossRef Google Scholar
Yi C, Jiang YM, Shi J, Qu HX, Xue S, Duan XW, Shi JY, Prasad NK (2010) ATP-regulation of antioxidant properties and phenolics in litchi fruit during browning and pathogen infection process. Food Chem 118:42–47.
https://doi.org/10.1016/j.foodchem.2009.04.074 CrossRef Google Scholar
Zhang W, Jiang B, Li W, Song H, Yu Y, Chen J (2009) Polyamines enhance chilling tolerance of cucumber (
L.) through modulating antioxidative system. Sci Hortic 122:200–208.
https://doi.org/10.1016/j.scienta.2009.05.013 CrossRef Google Scholar
Zhang H, Hu S, Zhang Z, Hu L, Jiang C, Wei Z, Liu J, Wang H, Jiang S (2011a) Hydrogen sulfide acts as a regulator of flower senescence in plants. Postharvest Biol Technol 60:251–257.
https://doi.org/10.1016/j.postharvbio.2011.01.006 CrossRef Google Scholar
Zhang Y, Li H, Shu W, Zhang C, Zhang W, Ye Z (2011b) Suppressed expression of ascorbate oxidase gene promotes ascorbic acid accumulation in tomato fruit. Plant Mol Biol Rep 29:638–645.
https://doi.org/10.1007/s11105-010-0271-4 CrossRef Google Scholar
Zhang D, Ren L, Yue JH, Wang L, Zhuo LH, Shen XH (2013) A comprehensive analysis of flowering transition in
ssp. orientalis (leighton) leighton by using transcriptomic and proteomic techniques. J Proteomics 80:1–25.
https://doi.org/10.1016/j.jprot.2012.12.028 CrossRef PubMed Google Scholar Copyright information
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