Effects of high temperature on in vitro tuberization and accumulation of stress-responsive proteins in potato
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Potato (Solanum tuberosum L.) plants are highly vulnerable to heat stress. Even moderately elevated temperatures can disturb the process of tuberization in this important crop, causing a decline in tuber initiation, a reduction in tuber bulking, and tuber disorders. In the present study, we investigated the effects of heat stress on tuberization in two potato cultivars, the heat-sensitive cultivar Désirée and the heat-tolerant cultivar Festival, using an in vitro system. A temperature of 29 °C reduced tuber initiation and tuber bulking, and stimulated shoot elongation in cv. Désirée, while this temperature treatment did not significantly alter tuberization or shoot elongation in cv. Festival. In addition, high temperature interfered with the onset of microtuber dormancy and promoted growth of tuber apical buds during the tuber bulking stage in both cultivars. Stress-responsive proteins HSP17.6-CI, HSP101, and eEF1A showed heat-induced accumulation patterns in shoots and microtubers of these two cultivars, with the exception of a decline in the abundance of eEF1A in cv. Désirée microtubers under heat stress. High levels of HSP17.6-CI in microtubers of cv. Désirée did not ameliorate the effects of heat stress on tuberization of this relatively heat-sensitive cultivar. Conversely, a higher level of eEF1A under heat stress in microtubers of the heat-tolerant cv. Festival indicated a possible function of this protein in alleviating the negative effects of high temperature on potato tuberization. This study suggested that analysis of stress-responsive proteins in potato microtubers combined with assessment of tuberization parameters in vitro may represent a useful screening procedure for selection of heat-tolerant potato genotypes.
KeywordsHeat stress Heat tolerance Solanum tuberosum HSP Eukaryotic elongation factor 1A
This study was funded by the Ministry of Education, Science and Technological Development of the Republic of Serbia, Project Grant No. TR31049.
- Delaplace P, Fauconnier ML, Sergeant K, Dierick JF, Oufir M, van der Wal F, America AHP, Renaut J, Hausman JF, du Jardin P (2009) Potato (Solanum tuberosum L.) tuber ageing induces changes in the proteome and antioxidants associated with the sprouting pattern. J Exp Bot 60:1273–1288CrossRefPubMedPubMedCentralGoogle Scholar
- FAO (2015) Statistical pocketbook: World Food and Agriculture. Food and Agriculture Organization of the United Nations, Rome, Italy. http://www.fao.org/3/a-i4691e.pdf. Accessed 07 Sept 2017
- Hancock RD, Morris WL, Ducreux LJM, Morris JA, Usman M, Verrall SR, Fuller J, Simpson CG, Zhang R, Hedley PE, Taylor MA (2014) Physiological, biochemical and molecular responses of the potato (Solanum tuberosum L.) plant to moderately elevated temperature. Plant Cell Environ 37:439–450CrossRefPubMedGoogle Scholar