Horticulture, Environment, and Biotechnology

, Volume 58, Issue 5, pp 479–487 | Cite as

Effects of methyl jasmonate on physicochemical qualities and internal browning of ‘queen’ pineapple fruit during cold storage

  • Panida BoonyaritthongchaiEmail author
  • Suriyan Supapvanich
Research Report


The effects of methyl jasmonate (MeJA) as a protectant against chilling injury on physicochemical qualities of ‘Trad-see-thong’ pineapple (‘Queen’ group) fruit was investigated. The pineapple fruit were immersed in 1 mM MeJA for 5 min and then stored at 10 ± 1°C for 20 days. MeJA had no effect on respiratory rate and pulp colour, but suppressed ethylene production during cold storage. Weight loss, ion leakage (IL), internal browning (IB), and loss of pulp firmness were inhibited by MeJA. The MeJA treatment also suppressed the reduction of lightness (L*) and yellowness (b*) and the increase of total phenols (TP) that commonly occurs during cold storage, and increased the polyphenol oxidase (PPO) activity of pulp adjacent to the core (PAC) tissue. Certain bioactive compounds, such as ascorbic acid (AsA) and superoxide dismutase (SOD) in PAC tissues were enhanced by MeJA. No significant difference in peroxidase (POD) activity in both pulp and PAC tissues were detected between the treated and control fruits. These results suggest that immersion in MeJA is an effective means to maintain quality, alleviate IB symptoms, and enhance certain bioactive compounds in ‘Queen’ pineapple fruit cv. Trad-see-thong during cold storage.

Additional key words

peroxidase Polyphenol oxidase postharvest quality Superoxide dismutase 


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Literature Cited

  1. Aghdam MS, Bodbodak, S. (2013) Physiological and biochemical mechanism regulating chilling tolerance in fruit and vegetables under postharvest salicylates and jasmonates treatments. Sci Hortic 156:73–85CrossRefGoogle Scholar
  2. Andrade Cuvi MJ, Vincente AR, Concellón A, Chaves AR (2011) Changes in red pepper antioxidants as affected by UV-C treatments and storage at chilling temperature. LWT -Food Sci Technol 44: 1666–1671CrossRefGoogle Scholar
  3. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 737:248–254CrossRefGoogle Scholar
  4. Brummell DA (2006) Cell wall disassembly in ripening fruit. Functional Plant Biol 33:103–119CrossRefGoogle Scholar
  5. Charles B, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44: 276–287CrossRefGoogle Scholar
  6. Cao SF, Zhen YH, Wang KT, Jin P, Rui HJ (2009) Methyl jasmonate reduces chilling injury and enhances antioxidant enzyme activity in postharvest loquat fruit. Food Chem 115:1458–1463CrossRefGoogle Scholar
  7. Cao S, Zhen Y, Wang K, Rui H, Tang S (2010) Effect of methyl jasmonate on cell wall modification of loquat fruit in relation to chilling injury after harvest. Food Chem 118:641–647CrossRefGoogle Scholar
  8. Department of Internal Trade of Thailand (2010) The trade situation of pineapple. Ministry of Commerce. agri_7_1053 pdf. Accessed 1 Febuary 2014.Google Scholar
  9. Ding CK, Wang CY, Gross KC, Smith DL (2001) Reduction of chilling injury and transcript accumulation of heat shock proteins in tomato fruit by methyl jasmonate and methyl salicylate. Plant Science 161:1153–1159CrossRefGoogle Scholar
  10. Ding CK, Wang CY, Gross KC, Smith DL (2002) Jasmonate and salicylate induce the expression of pathogenesis-related-protein genes and increase resistance to chilling injury in tomato fruit. Planta 214:895–901CrossRefPubMedGoogle Scholar
  11. Fan X, Mattheis JP, Fellman JK, Patterson ME (1997) Effect of methyl jasmonate on ethylene and volatile production by summered apples depends on fruit developmental stage. J Agric Food Chem 45: 208–211CrossRefGoogle Scholar
  12. FAO (2008) FAOSTAT database. aspx. Accessed 5 January 2011.Google Scholar
  13. Galeazzi MAM, Sgarbieri VC, Constantinides SM (1981) Isolation, purification and physicochemical characterization of polyphenoloxidases (PPO) from a dwarf variety of banana (Musa cavendishii L.). J Food Sci 46:150–155CrossRefGoogle Scholar
  14. González-Aguilar GA, Buta JG, Wang CY (2003) Methyl jasmonate and modified atmosphere packaging (MAP) reduce decay and maintain postharvest quality of papaya ‘Sunrise’. Postharvest Biol Technol 28:361–370CrossRefGoogle Scholar
  15. González-Aguilar GA, Fortiz J, Cruz R, Baez R, Wang CY (2000) Methyl jasmonate reduces chilling injury and maintains postharvest quality of mango fruit. J Agric Food Chem 48:515–519CrossRefPubMedGoogle Scholar
  16. González-Aguilar GA, Tiznado-Hernández ME, Zavaleta-Gatica R, Martínez-Téllez MA (2004) Methyl jasmonate treatments reduce chilling injury and activate the defense response of guava fruits. Biochem Biophys Res Commun 313:694–701CrossRefPubMedGoogle Scholar
  17. Hashimoto S, Yamafuji K (2001) The determination of diketo-L-gulonic acid, dehydro-L-ascorbic acid, and L-ascorbic acid in the same tissue extract by 2, 4-dinitrophenol hydrazine method. J Biol Chem 147:201–208Google Scholar
  18. Hong K, Xu H, Wang J, Zhang L, Hu H, Jia Z, Gu H, He Q, Gong D (2013) Quality changes and internal browning developments of summer pineapple fruit during storage at different temperature. Sci Hortic 151:68–74CrossRefGoogle Scholar
  19. Jin P, Zheng Y, Tang S, Rui H, Wang CY (2009) A combination of hot air and methyl jasmonate vapor treatment alleviates chilling injury of peach fruit. Postharvest Biol Technol 52:24–29CrossRefGoogle Scholar
  20. Lamb C, Dixon RA (1997) The oxidative burst in plant disease resistance. Annual Reveiw Plant Physiology. Plant Mol Biol 48 251–275Google Scholar
  21. Lim CS, Kang SM, Cho JL, Gross KC (2009) Antioxidizing enzyme activities in chilling-sensitive and chilling-tolerant pepper fruit as affected by stage of ripeness and storage temperature. J Am Soc Hortic Sci 134:156–163Google Scholar
  22. Lu X, Sun D, Li Y, Shi W, Sun G (2011). Pre-and post-harvest salicylic acid treatments alleviate internal browning and maintain quality of winter pineapple fruit. Scientia Horticulturae, 130:97–101CrossRefGoogle Scholar
  23. MacRae EA (1987) Development of chilling injury in New Zealand grown ‘Fuyu’ persimmon during storage. N Z J Exp Agric 15: 333–344Google Scholar
  24. Mayer AM (1987) Polyphenol oxidase and peroxidase in plants - recent progress. Phytochemistry 26:11–20CrossRefGoogle Scholar
  25. McCollum TG, McDonald RE (1991) Electrolyte leakage, respiration, and ethylene production as indices of chilling injury in grapefruit. HortScience 26:1191–1192Google Scholar
  26. Meir S, Philosoph-Hadas S, Lurie S, Droby S, Akerman M, Zauberman G, Shapiro B, Cohen E, Fuchs Y (1996) Reduction of chilling injury in stored avocado, grapefruit, and bell pepper by methyl jasmonate. Can J Bot 74:870–874CrossRefGoogle Scholar
  27. Meng X, Han J, Wang Q, Tian S (2009) Changes in physiology and quality of peach fruits treated by methyl jasmonate under low temperature stress. Food Chem 114:1028–1035CrossRefGoogle Scholar
  28. Nilprapruck P, Pradisthakarn N, Authanithee F, Keepjan P (2008) Effect of exogenous methyl jasmonate on chilling injury and quality of pineapple (Ananas comosus L.) cv. Pattavia. Silpakorn Univ Sci Tech J 2:33–42Google Scholar
  29. Nilprapruck P, Yodmingkhwan P (2009) Effect of exogenous methyl jasmonate on the internal browning of pineapple fruit (Ananas comosus L.) cv. Pattavia. Khon Kean Res J 14:489–497Google Scholar
  30. Paull RE, Rohrbach KG (1985) Symptom development of chilling injury in pineapple fruit. J Am Soc Hortic Sci 110:100–105Google Scholar
  31. Pusittigul I, Kondo S, Siriphanich J (2012) Internal browning of pineapple (Ananas comosus L.) fruit and endogenous concentrations of abscisic acid and gibberellins during low temperature storage. Sci Hortic 146:45–51CrossRefGoogle Scholar
  32. Promyou S, Supapvanich S, Boodkord B, Thangapiradeekajorn M (2012) Alleviation of chilling injury in jujube fruit (Ziziphus jujube Mill) by dipping in 35ºC water. Kasetsart J Nat Sci 46:107–119Google Scholar
  33. Quyen DTM, Jommwong A, Rachtanapun P (2013) Influence of storage temperature on ethanol content, microbial growth and other properties of queen pineapple fruit. Int J Agric Biol 15:207–214Google Scholar
  34. Raimbault AK, Marie-Alphonsine PA, Horry JP, Francois-Haugrin M, Romuald K, Soler A (2011) Polyphenol oxidase and peroxidase expression in four pineapple varieties (Ananas comosus L.) after a chilling injury. J Agric Food Chem 59:342–348CrossRefPubMedGoogle Scholar
  35. Saniewski M, Czapski J, Nowacki J, Lange E (1987a) The effect of methyl jasmonate on ethylene and 1-amiocyclopropane-1-carboxylic acid production in apple fruits. Biol Plant 29:199–203CrossRefGoogle Scholar
  36. Saniewski M, Nowacki J, Czapski J (1987b) The effect of methyl jasmonate on ethylene production and ethylene-forming enzyme activity in tomatoes. J Plant Physiol 129:175–180CrossRefGoogle Scholar
  37. Sayyari M, Babalar M, Kalantari S, Martínez-Romero D, Guillén F, Serrano M, Valero D (2011) Vapour treatments with methyl salicylic acid or methyl jasmonate alleviated chilling injury and enhanced antioxidant potential during postharvest storage of pomegranates. Food Chem 124:964–970CrossRefGoogle Scholar
  38. Selvarajah S, Bauchot AD, John P (2001) Internal browning in cold stored pineapples is suppressed by a postharvest application of 1-methylcyclopropene. Postharvest Biol Technol 23:167–170CrossRefGoogle Scholar
  39. Slinkard K, Singleton VL (1977) Total phenol analysis: automation and comparison with manual methods. Am J Enol Viticul 28:49–55Google Scholar
  40. Stewart RJ, Sawyer BJB, Bucheli CS, Robinson SP (2001) Polyphenol oxidase is induced by chilling and wouding in pineapple. Aust J Plant Physiol 28:181–191Google Scholar
  41. Supapvanich S, Pimsaga J, Srisujan P (2011) Physiochemical changes in fresh-cut wax apple (Syzygium samarangenese [Blume] Merrill & L.M. Perry) during storage. Food Chem 127:912–917PubMedGoogle Scholar
  42. Supapvanich S, Promyou S (2013) Efficiency of salicylic acid application on postharvest perishable crops. Salicylic Acid; Plant Growth and Development, XVIII, Hayat, Shamsul; Ahmad, Aqil; Alyemeni, Mohammed Nasser (Eds.), New York, Springer Publication.Google Scholar
  43. Supapvanich S, Tucker GA (2013) The effect of 1-methylcyclopropene (1-MCP) on quality and cell wall hydrolases activities of fresh-cut muskmelon (Cucumis melo var reticulates L.) during storage. Food Bioproc Tech 6:2196–2201CrossRefGoogle Scholar
  44. Wills R, McGlasson B, Graham D, Joyce D (2007) Postharvest, An Introduction to the Physiology and Handling of Fruit, Vegetables and Ornamentals, 5th, Wills Ron, McGlasson Barry, Graham Doug, Joyce Darly (Eds.), Sydney, University of New South Wales Press Ltd.Google Scholar
  45. Zhou Y, Dahler JM, Underhill SJR, Wills RBH (2003) Enzymes associated with blackheart development in pineapple fruit. Food Chem 80:565–572CrossRefGoogle Scholar

Copyright information

© Korean Society for Horticultural Science and Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Panida Boonyaritthongchai
    • 1
    • 2
    Email author
  • Suriyan Supapvanich
    • 2
    • 3
  1. 1.Postharvest Technology Program, School of Bioresources and TechnologyKing Mongkut’s University of Technology ThonburiThakham, Bangkhuntien District, BangkokThailand
  2. 2.Postharvest Technology Innovation CenterCommission of Higher EducationBangkokThailand
  3. 3.Department of Agricultural Education, Faculty of Industrial EducationKing Mongkut’s Institute of Technology LadkrabangBangkokThailand

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