Horticulture, Environment, and Biotechnology

, Volume 58, Issue 2, pp 144–151 | Cite as

Pre-harvest 1-methylcyclopropene application affects post-harvest physiology and storage life of the cut rose cv. Carola

  • Shuai Huang
  • Bi Gong
  • Fujuan Wei
  • Huiling MaEmail author
Research Report Postharvest Technology


We evaluated and optimized the effects of an alternative pre-harvest application of the non-toxic ethylene action inhibitor 1-methylcyclopropene (1-MCP)on the physiology and storage life of the cut rose (Rosa hybrida) cv. Carola. Cut rose was treated with a single pre-harvest liquid formula spray of 10 μL·L-11-MCP or a single treatment of a modified atmosphere packaging of 50-μm thick polyethylene film (PE50), or a combination of the two (1-MCP+PE50), and the effects on vase life, storage life, and cold storage senescence related physiological changes were evaluated. Cut rose subjected to the pre-harvest 1-MCP spray treatment had the longest storage life (26 days), while those subjected to PE50, 1-MCP+PE50, and the untreated control treatment had mean storage lives of 22, 19, and 16 days, respectively. Additionally, the pre-harvest 1-MCP spray treatment significantly extended the vase life of cold-stored flowers for zero or six days, by 27.08% and 42.55%, respectively. The pre-harvest spray treatment significantly suppressed respiration intensity, ethylene production, soluble protein degradation, 1-aminocyclopropane-1-carboxylic acid (ACC) production, and ACC oxidase activity. The 1-MCP+PE50 treatment compromised all the effects of the 1-MCP treatment except the effect on soluble protein content. We conclude that a pre-harvest 1-MCP spray treatment has positive effects on the vase life and storage longevity of cut rose, and that combining this treatment with a PE50 package diminishes these effects.

Additional key words

Ethylene regulation Preservation Rosa hybrida 


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Literature Cited

  1. Anderson JW, Rowan KS (1966) Activity of aminoacyl-transfer-ribonucleic acid synthetases in tobacco-leaf tissue in relation to senescence and to the action of 6-furfurylaminopurine. Biochem J 101:15–18CrossRefPubMedPubMedCentralGoogle Scholar
  2. Argenta L, Fan X, Mattheis J (2000) Delaying establishment of controlled atmosphere or CO2 exposure reduces ‘Fuji’ apple CO2 injury without excessive fruit quality loss. Postharvest Biol Technol 20:221–229CrossRefGoogle Scholar
  3. Argenta LC, Mattheis JP, Fan X (2010) Interactive effects of CA storage, 1-methylcyclopropene and methyl jasmonate on quality of apple fruit. Acta Hortic 857:259–266CrossRefGoogle Scholar
  4. Arrom L, Munné-Bosch S (2012) Sucrose accelerates flower opening and delays senescence through a hormonal effect in cut lily flowers. Plant Sci 188–189:41–47CrossRefPubMedGoogle Scholar
  5. Axelrod B, Cheesbrough TM, Laakso S (1981) Lipoxygenase from soybeans: ec linoleate:oxygen oxidoreductase. Methods Enzymol 71:441–451CrossRefGoogle Scholar
  6. Batista RJDR, Grossi JAS, Ribeiro Junior JI, Barbosa JG, Finger FL (2009) Rose flower longevity in response to ethylene and 1-methylcyclopropene (1-mcp). Acta Hortic 847:363–368CrossRefGoogle Scholar
  7. Bessemans N, Verboven P, Verlinden BE, Nicolaï BM (2016). A novel type of dynamic controlled atmosphere storage based on the respiratory quotient (rq-dca). Postharvest Biol Technol 115:91–102CrossRefGoogle Scholar
  8. Borochov A, Drori A, Tirosh T, Borochov-Neori H, Mayak S (1990) Quantitative and qualitative changes in membrane proteins during petal senescence. J Plant Physiol 136:203–207CrossRefGoogle Scholar
  9. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRefPubMedGoogle Scholar
  10. Chang YCA, Lin WL, Hou JY, Yen WY, Lee N (2013) Concentration of 1-methylcyclopropene and the duration of its application affect anti-ethylene protection in Phalaenopsis. Sci Hortic 153:117–123CrossRefGoogle Scholar
  11. Cock LS, Valenzuela LST, Aponte AA (2013) Physical, chemical and sensory changes of refrigerated yellow pitahaya treated preharvest with 1-MCP. Dyna 80:11–20, ISSN: 0012-7353Google Scholar
  12. Cohen AS, Popovic RB, Zalik S (1979) Effects of Polyamines on Chlorophyll and Protein Content, Photochemical Activity, and Chloroplast Ultrastructure of Barley Leaf Discs during Senescence. Plant Physiol 64:717–720CrossRefPubMedPubMedCentralGoogle Scholar
  13. Daneshi Nergi MA, Ahmadi N (2014) Effects of 1-MCP and ethylene on postharvest quality and expression of senescence-associated genes in cut rose cv. Sparkle. Sci Hortic 166:78–83CrossRefGoogle Scholar
  14. Devecchi M, Meeteren JPJ, Woltering EJ (2003) Effects of low O2 on cut rose flowers at suboptimal temperature. Acta Hortic 628:855–861CrossRefGoogle Scholar
  15. Elfving DC, Drake SR, Reed AN, Visser DB (2007) Preharvest applications of sprayable 1-methylcyclopropene in the orchard for management of apple harvest and postharvest condition. HortScience 42:1192–1199, ISSN: 0018-5345Google Scholar
  16. Fawbush F, Nock JF, Watkins CB (2008) External carbon dioxide injury and 1-methylcyclopropene (1-MCP) in the ‘Empire’ apple. Postharvest Biol Technol 48:92–98CrossRefGoogle Scholar
  17. Freiman ZE, Rodov V, Yablovitz Z, Horev B, Flaishman MA (2012) Preharvest application of 1-methylcyclopropene inhibits ripening and improves keeping quality of ‘Brown Turkey’ figs (Ficus carica L.). Sci Hortic 138:266–272CrossRefGoogle Scholar
  18. Hershkovitz V, Saguy SI, Pesis E (2005) Postharvest application of 1-MCP to improve the quality of various avocado cultivars. Postharvest Biol Technol 37:252–264CrossRefGoogle Scholar
  19. Hunter DA, Yi M, Xu X, Reid MS (2004) Role of ethylene in perianth senescence of daffodil (Narcissus pseudonarcissus L. ‘Dutch Master’). Postharvest Biol Technol 32:269–280CrossRefGoogle Scholar
  20. Hyodo H, Nishino T (1981) Wound-induced Ethylene Formation in Albedo Tissue of Citrus Fruit. Plant Physiol 67:421–423CrossRefPubMedPubMedCentralGoogle Scholar
  21. Imsabai W, Ketsa S, Van Doorn WG (2010) Role of ethylene in the lack of floral opening and in petal blackening of cut lotus (Nelumbo nucifera) flowers. Postharvest Biol Technol 58:57–64CrossRefGoogle Scholar
  22. Jin J, Shan N, Ma N, Bai J, Gao J (2006) Regulation of ascorbate peroxidase at the transcript level is involved in tolerance to postharvest water deficit stress in the cut rose (Rosa hybrida L.) cv. Samantha. Postharvest Biol Technol 40:236–243CrossRefGoogle Scholar
  23. Jung SK, Watkins CB (2011) Involvement of ethylene in browning development of controlled atmosphere-stored ‘Empire’ apple fruit. Postharvest Biol Technol 59:219–226CrossRefGoogle Scholar
  24. Kou L, Turner ER, Luo Y (2012) Extending the shelf life of edible flowers with controlled release of 1-methylcyclopropene and modified atmosphere packaging. J Food Sci 77:188–193CrossRefGoogle Scholar
  25. Liao WB, Zhang ML, Yu JH (2013) Role of nitric oxide in delaying senescence of cut rose flowers and its interaction with ethylene. Sci Hortic 155:30–38CrossRefGoogle Scholar
  26. Lizada MCC, Yang SF (1979) A simple and sensitive assay for 1-aminocyclopropane-1-carboxylic acid. Anal Biochem 100:140–145CrossRefPubMedGoogle Scholar
  27. Macnish AJ, Joyce DC, Hofman PJ, Simons DH, Reid MS (2000) 1-methylcyclopropene treatment efficacy in preventing ethylene perception in banana fruit and grevillea and waxflower flowers. Aust J Exp Agric 40:471–481CrossRefGoogle Scholar
  28. Müller R, Sisler EC, Serek M (2000) Stress induced ethylene production, ethylene binding, and the response to the ethylene action inhibitor 1-MCP in miniature roses. RSci Hortic 83:51–59CrossRefGoogle Scholar
  29. Nakatsuka A, Shiomi S, Kubo Y, Inaba A (1997) Expression and internal feedback regulation of ACC synthase and ACC oxidase genes in ripening tomato fruit. Plant Cell Physiol 38:1103–1110CrossRefPubMedGoogle Scholar
  30. Paliyath G, Murr DP, Handa AK, Lurie S (2008) Postharvest biology and technology of fruits, vegetable and flowers. Wiley Black Well Publishing, Hoboken, New Jersey, USAGoogle Scholar
  31. Pascale SD, Maturi T, Nicolais V (2005) Modified atmosphere packaging (MAP) for preserving gerbera, lilium and rosa cut flowers. Acta Hortic 682:1145–1152CrossRefGoogle Scholar
  32. Perik RRJ, Razé D, Ferrante A, Doorn WGV (2014) Stem bending in cut Gerbera jamesonii, flowers: Effects of a pulse treatment with sucrose and calcium ions. Postharvest Biol Technol 98:7–13CrossRefGoogle Scholar
  33. Scariot V, Paradiso R, Rogers H, Pascale SD (2014) Ethylene control in cut flowers: Classical and innovative approaches. Postharvest Biol Technol 97:83–92CrossRefGoogle Scholar
  34. Serek M, Sisler EC, Reid MS (1994) Novel gaseous ethylene binding inhibitor prevents ethylene effects in potted flowering plants. J. 119:1230–1233, ISSN: 0003-1062Google Scholar
  35. Shahri W, Tahir I (2011) Flower Senescence-strategies and some associated events. Bot Rev 77:152–184CrossRefGoogle Scholar
  36. Sisler EC, Dupille E, Serek M (1996) Effect of 1-methylcyclopopene and methylenecyclopropane on ethylene binding and ethylene action on cut carnations. Plant Growth Regul 18:79–86CrossRefGoogle Scholar
  37. Sisler EC, Serek M (1997) Inhibitors of ethylene responses in plants at the receptor level: recent developments. Physiol. Plant 100:577–582Google Scholar
  38. Song L, Liu H, Su X, You Y, Jiang Y (2006) Effects of adenosine triphosphate on the vase life of cut carnation flowers. 46:137–139Google Scholar
  39. Sood S, Vyas D, Nagar PK (2006) Physiological and biochemical studies during flower development in two rose species. 108:390–396Google Scholar
  40. Vonabrams GJ, Pratt HK (1968) Effect of the kinetin-naphthaleneacetic acid interaction upon total RNA and protein in senescing detached leaves. Plant Physiol 43:1271–1278CrossRefPubMedPubMedCentralGoogle Scholar
  41. Wang QG, Mou WL, Chen QM June 2, 2010. 1-methylcyclopropene liquid formula. China patent Application No. CN200910230685.8Google Scholar
  42. Watkins CB, Nock JF (2012) Rapid 1-methylcyclopropene (1-MCP) treatment and delayed controlled atmosphere storage of apples. 69:24–31Google Scholar
  43. Watkins CB, Silsby KJ, Goffinet MC (1997) Controlled Atmosphere and Antioxidant Effects on External CO2 Injury of ‘Empire’ Apples. Hortsci 32:1242–1246, ISSN: 0018-5345Google Scholar
  44. Woltering EJ, van Doorn WG (1988) Role of ethylene in senescence of petals morphological and taxonomical relationships. 39:1605–1616Google Scholar
  45. Yamane K, Yamaki Y, Fujishige N (2004) Effects of exogenous ethylene and 1-MCP on ACC oxidase activity, ethylene production and vase life in Cattleya alliances. J Jpn Soc Hortic Sci 73:128–133CrossRefGoogle Scholar
  46. Yuan RC, Carbaugh DH (2007) Effects of NAA, AVG, and 1-MCP on ethylene biosynthesis, preharvest fruit drop, fruit maturity, and quality of ‘Golden Supreme’ and ‘Golden Delicious’ apples. HortScience 42:101–105, ISSN: 0018-5345Google Scholar
  47. Zhang F, Wang Y, Li L, Liu T (2013) Effects of phosphine fumigation on postharvest quality of four Chinese cut flower species. Postharvest Biol Technol 86:66–72CrossRefGoogle Scholar

Copyright information

© Korean Society for Horticultural Science and Springer-Verlag GmbH 2017

Authors and Affiliations

  1. 1.College of Life ScienceNorthwest A&F UniversityYangling, ShaanxiChina
  2. 2.College of Architecture and ArtNorthwest A&F UniversityYangling, ShaanxiChina

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