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Horticulture, Environment, and Biotechnology

, Volume 58, Issue 1, pp 66–77 | Cite as

Transcript profiling of native Korean grapevine species Vitis flexuosa exposed to dehydration and rehydration treatment

  • Jin Seok Moon
  • Youn Young HurEmail author
  • Sung-Min Jung
  • Youn-Jung Choi
  • Jong-Chul Nam
  • Jeong-Gwan Park
  • Sang Wook Koh
Research Report

Abstract

Vitis flexuosa, a native Korean wild grapevine species, is more tolerant to abiotic stress than cultivated grapevines, but little is known about the mechanism underlying this tolerance. To better characterize the responses of V. flexuosa to drought stress and subsequent rehydration, we treated V. flexuosathe plants with four stress conditions: short- and long-term dehydration, rehydration immediately after long-term drought, and long-term rehydration after long-term drought. We examined gene expression profiles in response to these treatments using whole-genome microarray analysis at 12X coverage (using the NimbleGen protocol). We identified a 5,964 genes that were differentially expressed between the stress treatments and the well-watered control. Gene ontology analysis showed that most of the differentially expressed genes (DEGs), regardless of treatment, participate in the metabolism of proteins or phosphatecontaining compounds and function in the binding of metal ions, as kinases, or as phosphotransferases. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that genes involved in secondary metabolite biosynthesis were downregulated during dehydration but upregulated during rehydration. Among the DEGs encoding transcription factors, genes in the MYB family were the most common differentially genes in all treatment groups. The NAC family was the second most common group during dehydration, while the AP2/ERF family was the second most common during rehydration. These results will help elucidate the mechanisms underlying the response of V. flexuosa to drought stress and will be valuable for breeding drought-tolerant grapevines.

Additional key words

differentially expressed gene gene ontology KEGG pathway analysis transcription factor 

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Copyright information

© Korean Society for Horticultural Science and Springer-Verlag GmbH 2017

Authors and Affiliations

  • Jin Seok Moon
    • 1
  • Youn Young Hur
    • 1
    Email author
  • Sung-Min Jung
    • 1
  • Youn-Jung Choi
    • 2
  • Jong-Chul Nam
    • 1
  • Jeong-Gwan Park
    • 1
  • Sang Wook Koh
    • 3
  1. 1.Fruit Research Division, Institute of Horticultural and Herbal ScienceRural Development AdministrationWanjuRepublic of Korea
  2. 2.Floriculture Research Division, Institute of Horticultural and Herbal ScienceRural Development AdministrationWanjuRepublic of Korea
  3. 3.Research Institute of Climate Change and Agriculture, Institute of Horticultural and Herbal ScienceRural Development AdministrationJejuRepublic of Korea

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