Horticulture, Environment, and Biotechnology

, Volume 58, Issue 1, pp 12–20 | Cite as

Blue and red light-emitting diodes improve the growth and physiology of in vitro-grown carnations ‘Green Beauty’ and ‘Purple Beauty’

  • Abinaya Manivannan
  • Prabhakaran Soundararajan
  • Yoo Gyeong Park
  • Hao Wei
  • Soo Hoon Kim
  • Byoung Ryong JeongEmail author
Research Report


The objective of this study was to determine the effect of light quality on the growth, physiology, and antioxidant enzyme activity of two important domestic carnation (Dianthus caryophyllus) cultivars: ‘Green Beauty’ and ‘Purple Beauty’. In vitro-grown shoot tip explants were cultured on the plant growth regulator (PGR)-free Murashige and Skoog (MS) medium under a conventional cool white fluorescent lamp (control), blue light-emitting diode (LED), or red LED. Growth traits, photosynthetic and biochemical parameters, activity of antioxidant enzymes, and nutrient content were measured after 8 weeks. Interestingly, the blue and red LED treatments resulted in a significant increase in growth, photosynthetic parameters, and nutrient content in comparison to the conventional cool white florescent lamp treatment. In addition, red LED treatment increased the activities of antioxidant enzymes and elemental contents in both cultivars. Thus, incorporating blue or red LED treatments enhances the quality of ‘Green Beauty’ and ‘Purple Beauty’ carnations propagated in vitro.

Additional key words

antioxidant enzymes macronutrients micronutrients photosynthesis reactive oxygen species scanning electron microscopy stomata 


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Literature Cited

  1. Arney SE, Mitchell DL (1969) The effect of abscisic acid on stem elongation and correlative inhibition. New Phytol 68: 1001–1015CrossRefGoogle Scholar
  2. Baque, MA, Elgirban A, Lee EJ, Paek KY (2012) Sucrose regulated enhanced induction of anthraquinone, phenolics, flavonoids biosynthesis and activities of antioxidant enzymes in adventitious root suspension cultures of Morinda citrifolia L. Acta Physiol Plant 34: 405–415CrossRefGoogle Scholar
  3. Barker AV, Pilbeam DJ (2015) Handbook of plant nutrition. CRC press, Florida, USAGoogle Scholar
  4. Bienfait HF (1985) Regulated redox processes at the plasmalemma of plant root cells and their function in iron uptake. J Bioenerg Biomembr 17: 73–83CrossRefPubMedGoogle Scholar
  5. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254.CrossRefPubMedGoogle Scholar
  6. Cakmak I, Marschner H (1992) Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. Plant Physiol 98: 1222–1227CrossRefPubMedPubMedCentralGoogle Scholar
  7. Cakmak I, Marschner H, Bangerth F (1989) Effect of zinc nutritional status on growth, protein metabolism and levels of indole-3-acetic acid and other phytohormones in bean (Phaseolus vulgaris L.). J Exp Bot 40: 405–412CrossRefGoogle Scholar
  8. Chatterjee M, Tabi Z, Galli MS, Malcomber A, Buck M, Muszynski S, Gallavotti A (2014) The boron efflux transporter ROTTEN EAR is required for maize inflorescence development and fertility. Plant Cell 26: 2962–2977CrossRefPubMedPubMedCentralGoogle Scholar
  9. Christou A, Manganaris GA, Fotopoulos V (2014) Systemic mitigation of salt stress by hydrogen peroxide and sodium nitroprusside in strawberry plants via transcriptional regulation of enzymatic and non-enzymatic antioxidants. Environ Exp Bot 107: 46–54CrossRefGoogle Scholar
  10. Correll MJ, Wu Y, Weathers PJ (2000) Controlling hyperhydration of carnations Dianthus caryophyllus L. grown in a mist reactor. Biotechnol Bioeng 71: 307–314Google Scholar
  11. Daud N, Faizal A, Geelen D (2013) Adventitious rooting of Jatropha curcas L. is stimulated by phloroglucinol and by red LED light. In Vitro Cell Dev Biol Plant 49: 183–190CrossRefGoogle Scholar
  12. Falk KL, Tokuhisa JG, Gershenzon J (2007) The effect of sulfur nutrition on plant glucosinolate content: Physiology and molecular mechanisms. Plant Biol 9: 573–581CrossRefPubMedGoogle Scholar
  13. Fankhauser C, Chory J (1997) Light control of plant development. Annu Rev Cell Dev Biol 13: 203–229CrossRefPubMedGoogle Scholar
  14. Giannopolitis CN, Ries SK (1977) Superoxide dismutases. Plant Physiol 59: 309–314CrossRefPubMedPubMedCentralGoogle Scholar
  15. Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48: 909–930CrossRefPubMedGoogle Scholar
  16. Gransee A, Fuhrs H (2013) Magnesium mobility in soils as a challenge for soil and plant analysis, magnesium fertilization and root uptake under adverse growth conditions. Plant Soil 368: 5–21CrossRefGoogle Scholar
  17. Gupta SD, Sahoo TK (2015) Light emitting diode (LED)-induced alteration of oxidative events during in vitro shoot organogenesis of Curculigo orchioides Gaertn. Acta Physiol Plant 37: 1–9CrossRefGoogle Scholar
  18. Hahn EJ, Kozai T, Paek KY (2000) Blue and red light-emitting diodes with or without sucrose and ventilation affect in vitro growth of Rehmannia glutinosa plantlets. J Plant Biol 43: 247–250CrossRefGoogle Scholar
  19. Heo J, Lee C, Chakrabarty D, Paek K (2002) Growth responses of marigold and salvia bedding plants as affected by monochromic or mixture radiation provided by a light-emitting diode (LED). Plant Growth Regul 38: 225–230CrossRefGoogle Scholar
  20. Johkan M, Shoji K, Goto F, Hashida S, Yoshihara T (2010) Blue light-emitting diode light irradiation of seedlings improves seedling quality and growth after transplanting in red leaf lettuce. HortScience 45: 1809–1814Google Scholar
  21. Kaiser BN, Gridley KL, Brady JN, Phillips T, Tyerman SD (2005) The role of molybdenum in agricultural plant production. Ann Bot 96: 745–754CrossRefPubMedPubMedCentralGoogle Scholar
  22. Kim SJ, Hahn EJ, Heo JW (2004) Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro. Sci Hortic 101: 143–151CrossRefGoogle Scholar
  23. Konieczny R, Banas AK, Surówka E, Michalec Z, Miszalski Z, Libik-Konieczny M (2014) Pattern of antioxidant enzyme activities and hydrogen peroxide content during developmental stages of rhizogenesis from hypocotyl explants of Mesembryanthemum crystallinum L. Plant Cell Rep 33: 165–177CrossRefPubMedGoogle Scholar
  24. Kumawat S, Kachhwaha S, Kothari SL (2013) Micronutrient optimization in the basal medium improves in vitro plant regeneration in carnation (Dianthus caryophyllus L.) and Chinese pink (Dianthus chinensis L.). Propag Ornam Plants 13: 3–11Google Scholar
  25. Lin Y, Li J, Li B, He T, Chun Z (2011) Effects of light quality on growth and development of protocorm-like bodies of Dendrobium officinale in vitro. Plant Cell Tissue Organ Cult 105: 329–335CrossRefGoogle Scholar
  26. Lu Z, Quinones M.A, Zeiger E (1993) Abaxial and adaxial stomata from Pima cotton (Gossypium barbadense L.) differ in their pigment content and sensitivity to light quality. Plant Cell Environ 16: 851–858CrossRefGoogle Scholar
  27. Manivannan A, Soundararajan P, Halimah N, Ko CH, Jeong BR (2015a) Blue LED Light enhances growth, phytochemical contents, and antioxidant enzyme activities of Rehmannia glutinosa cultured in vitro. Hortic Environ Biotechnol 56: 105–113CrossRefGoogle Scholar
  28. Manivannan A, Jana S, Soundararajan P, Ko CH, Jeong BR (2015b) Antioxidant enzymes metabolism and cellular differentiation during the developmental stages of somatic embryogenesis in Torilis japonica (Houtt.) DC. Plant Omics 8: 461–471Google Scholar
  29. Marschner H (1995) Mineral nutrition of higher plants. Ed 2, Academic Press, San Diego, CA, USAGoogle Scholar
  30. Mengxi L, Zhigang X, Yang Y, Yijie F (2011) Effects of different spectral lights on Oncidium PLBs induction, proliferation, and plant regeneration. Plant Cell Tissue Organ Cult 106: 1–10CrossRefGoogle Scholar
  31. Mohamed AH (2011) A protocol for the mass-micropropagation of carnation (Dianthus caryophyllus L.). J Horticult Sci Biotechnol 86: 135–140CrossRefGoogle Scholar
  32. Mujib A, Tonk D, Ali M (2014) Plant regeneration from protoplasts in Indian local Coriandrumsativum L.: Scanning electron microscopy and histological evidences for somatic embryogenesis. Plant Cell Tissue Organ Cult 117: 323–334CrossRefGoogle Scholar
  33. Muneer S, Kim EJ, Jeong SP, Jeong HL (2014) Influence of green, red and blue light emitting diodes on multiprotein complex proteins and photosynthetic activity under different light intensities in lettuce leaves (lactuca sativa L.). Int J Mol Sci 15: 4657–4670CrossRefPubMedPubMedCentralGoogle Scholar
  34. Murashige T, Skoog F (1962) A revised medium of rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  35. Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22: 867–880Google Scholar
  36. Nhut DT, Takamura T, Watanabe H (2003) Responses of strawberry plantlets cultured in vitro under superbright red and blue light emitting diodes (LEDs). Plant Cell Tissue Organ Cult 73: 43–52CrossRefGoogle Scholar
  37. Pedas P, Stokholm MS, Hegelund JN, Ladegård AH, Schjoerring JK, Husted S (2014) Golgi localized barley MTP8 proteins facilitate Mn transport. PLoS ONE 9, e113759CrossRefPubMedPubMedCentralGoogle Scholar
  38. Poudel PR., Kataoka I, Mochioka R (2008) Effect of red-and blue-light-emitting diodes on growth and morphogenesis of grapes Plant Cell Tissue Organ Cult 92: 147–153Google Scholar
  39. Rodríguez N, Menéndez N, Tornero J, Amils R, De La Fuente V (2005) Internal iron biomineralization in Imperata cylindrica, a perennial grass: Chemical composition, speciation and plant localization. New Phytol 165: 781–789CrossRefPubMedGoogle Scholar
  40. Samuoliene G, Sirtautas R, Brazaityte A, Duchovskis P (2012) LED lighting and seasonality effects antioxidant properties of baby leaf lettuce. Food Chem 134: 1494–1499CrossRefPubMedGoogle Scholar
  41. Shah K, Kumar RG, Verma S, Dubey RS (2001) Effect of cadmium on lipid peroxidation, superoxide anion generation and activities of antioxidant enzymes in growing rice seedlings. Plant Sci 161: 1135–1144CrossRefGoogle Scholar
  42. Sharkey TD, Raschke K (1981) Effect of light quality on stomatal opening in leaves of Xanthium strumarium L. Plant Physiol 68: 1170–1174CrossRefPubMedPubMedCentralGoogle Scholar
  43. Sharma P, Jha AB, Dubey RS, Pessarakli M (2012) Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. J Bot 1–26Google Scholar
  44. Shohael AM, Ali MB, Yu KW, Hahn EJ, Islam R, Paek KY (2006) Effect of light on oxidative stress, secondary metabolites and induction of antioxidant enzymes in Eleutherococcus senticosus somatic embryos in bioreactor. Process Biochem 41: 1176–1185Google Scholar
  45. Sivanesan I, Son MS, Soundararajan P, Jeong BR (2014) Effect of silicon on growth and temperature stress tolerance of Nephrolepis exaltata ‘Corditas’. Kor J Hort Sci 32: 142–148Google Scholar
  46. Soundararajan P, Manivannan A, Park YG, Muneer S, Jeong BR (2015) Silicon alleviates salt stress by modulating antioxidant enzyme activities in Dianthus caryophyllus ‘Tula’. Hortic Environ Biotechnol 56: 233–239CrossRefGoogle Scholar
  47. Tian M, Gu Q, Zhu M (2003) The involvement of hydrogen peroxide and antioxidant enzymes in the process of shoot organogenesis of strawberry callus. Plant Sci 165: 701–707CrossRefGoogle Scholar
  48. Toyomasu T, Tsuji H, Yamane H, Nakayama M, Yamaguchi I, Murofushi N, Takahashi N, Inoue Y (1993) Light effects on endogenous levels of gibberellins in photoblastic lettuce seeds. J Plant Growth Regul 12: 85–90CrossRefGoogle Scholar
  49. Triques K, Rival A, Beulé T, Puard M, Roy J, Nato A, Lavergne D, Havaux M, Verdeil JL, Sangare A, Hamon S (1997) Photosynthetic ability of in vitro grown coconut (Cocos nucifera L.) plantlets derived from zygotic embryos. Plant Sci 127: 39–51CrossRefGoogle Scholar
  50. Vallee BL, Auld DS (1990) Zinc coordination, function, and structure of zinc enzymes and other proteins. Biochemistry 29: 5647–5659CrossRefPubMedGoogle Scholar
  51. van Altvorst AC, Koehorst HJJ, Bruinsma T, Jansen J, Custers JBM, Jong JD, Dons JJM (1992) Adventitious shoot formation from in vitro leaf explants of carnation (Dianthus caryophyllus L.). Sci Hortic 51: 223–235CrossRefGoogle Scholar
  52. Wang Y, Wu WH (2013) Potassium transport and signaling in higher plants. Annu Rev Plant Biol 64: 451–476CrossRefPubMedGoogle Scholar
  53. Wheeler RM, Mackowiak CL, Sager JC (1991) Soybean stem growth under high-pressure sodium with supplemental blue lighting. Agron J 83: 903–906CrossRefPubMedGoogle Scholar
  54. Yin L, Wang S, Li J, Tanaka K, Oka M (2013) Application of silicon improves salt tolerance through ameliorating osmotic and ionic stresses in the seedling of Sorghum bicolor. Acta Physiol Plant 35: 3099–3107CrossRefGoogle Scholar
  55. Zeiger E, Field C (1982) Photocontrol of the functional coupling between photosynthesis and stomatal conductance in the intact leaf blue light and par-dependent photosystems in guard cells. Plant Physiol 70: 370–375CrossRefPubMedPubMedCentralGoogle Scholar
  56. Zhan L, Li Y, Hu J, Pang L, Fan H (2012) Browning inhibition and quality preservation of fresh-cut romaine lettuce exposed to high intensity light. Innov Food Sci Emerg Technol 14: 70–76CrossRefGoogle Scholar

Copyright information

© Korean Society for Horticultural Science and Springer-Verlag GmbH 2017

Authors and Affiliations

  • Abinaya Manivannan
    • 1
  • Prabhakaran Soundararajan
    • 1
  • Yoo Gyeong Park
    • 2
  • Hao Wei
    • 1
  • Soo Hoon Kim
    • 1
  • Byoung Ryong Jeong
    • 1
    • 2
    • 3
    Email author
  1. 1.Division of Applied Life Science (BK21 Plus), Graduate SchoolGyeongsang National UniversityJinjuKorea
  2. 2.Institute of Agriculture and Life ScienceGyeongsang National UniversityJinjuKorea
  3. 3.Research Institute of Life ScienceGyeongsang National UniversityJinjuKorea

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