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Horticulture, Environment, and Biotechnology

, Volume 57, Issue 4, pp 392–403 | Cite as

Comparative analysis of individual glucosinolates, phytochemicals, and antioxidant activities in broccoli breeding lines

  • Jung Su Jo
  • Shiva Ram Bhandari
  • Gwan Ho Kang
  • Jun Gu Lee
Research Report Genetics and Breeding

Abstract

The aim of this research was to evaluate the profile and concentration of individual glucosinolates (GSL), and the total phenol content (TPC), total flavonoid content (TFC), ascorbic acid content, and antioxidant activity of broccoli florets and flower stalks (10 commercial cultivars, 19 F1 hybrids, and 20 inbred lines). All broccoli heads were harvested at their marketable stage, and their flower stalks and florets were subjected to phytochemical analysis. GSL, TPC, TFC, and ascorbic acid content varied significantly depending on broccoli genotype. Altogether, nine GSLs were identified, four of which (glucoraphanin, progoitrin, glucoerucin, and glucobrassicin) were the most common in both broccoli flower stalks and florets. In florets, glucobrassicin was the most abundant GSL (4.46 μmol·g-1 DW), followed by glucoraphanin (1.93 μmol·g-1 DW), whereas glucoraphanin was the most abundant in flower stalks (1.47 μmol·g-1 DW). The concentrations of total GSLs, TPC, and TFC in florets were relatively higher than those in the flower stalks, whereas the concentration of ascorbic acid was higher in the flower stalks than the florets. Almost all F1 hybrids and inbred lines exhibited higher TPC, TFC, ascorbic acid concentration, and antioxidant activities than those in the commercial cultivars. Three F1 hybrids; 5075, 5078, and 5079, and one inbred line (5308) had the highest glucoraphanin and total GSL content. Three inbred lines, 5307, 5311, and 5409 had the higher concentration of glucobrassicin and total GSLs, superior antioxidant activity with low PRO+EPI content. These results suggest that these genotype selections had desirable compositions of individual GSLs and higher nutritional value for commercialization as functional vegetables.

Additional key words

ascorbic acid flavonoid glucobrassicin glucoraphanin phenolic plant part 

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Literature Cited

  1. Bhandari SR, Jo JS, Lee JG (2015) Comparison of glucosinolate profiles in different tissues of nine Brassica crops. Molecules 20:15827–15841CrossRefPubMedGoogle Scholar
  2. Bhandari SR, Kwak JH (2014) Seasonal variation in phytochemicals and antioxidant activities in different tissues of various broccoli cultivars. Afr J Biotechnol 13:604–615CrossRefGoogle Scholar
  3. Bhandari SR, Kwak JH (2015a) Chemical composition and antioxidant activity in different tissues of Brassica vegetables. Molecules 20:1228–1243CrossRefPubMedGoogle Scholar
  4. Bhandari SR, Kwak JH (2015b) Seasonal variation in contents of sugars in different parts of broccoli. Korean J Hortic Sci Technol 33:276–282CrossRefGoogle Scholar
  5. Bonnesen C, Eggleston IM, Hayes JD (2001). Dietary indoles and isothiocyanates that are generated from cruciferous vegetables can both stimulate apoptosis and confer protection against DNA damage in human colon cell lines. Cancer Res 61:6120–6130Google Scholar
  6. Brown AF, Yousef GG, Jeffery EH, Klein BP, Wallig MA, Kushad MM, Juvik JA (2002) Glucosinolate profiles in broccoli: variation in levels and implications in breeding for cancer chemoprotection. J Am Soc Hortic Sci 127:807–813Google Scholar
  7. Cartea ME, Francisco M, Soengas P, Velasco P (2011) Phenolic compounds in Brassica vegetables. Molecules 16:251–280CrossRefGoogle Scholar
  8. Chung FL, Conaway CC, Rao CV, Reddy BS (2000) Chemoprevention of colonic aberrant crypt foci in Fischer rats by sulforaphane and phenethyl isothiocyanate. Carcinogenesis 21:2287–2291CrossRefPubMedGoogle Scholar
  9. Clarke DB (2010) Glucosinolates, structures and analysis in food. Anal Methods 2:310–325CrossRefGoogle Scholar
  10. Dinkova-Kostova AT, Kostov RV (2012) Glucosinolates and isothiocyanates in health and disease. Trends Mol Med 18:337–347CrossRefPubMedGoogle Scholar
  11. Fahey JW, Zalcmann AT, Talalay P (2001) The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochemistry 56:5–51CrossRefPubMedGoogle Scholar
  12. Gliszczynska-Swiglo A, Ciska E, Pawlak-Lemanska K, Chmielewski J, Borkowski T, Tyrakowska B (2006) Changes in the content of health-promoting compounds and antioxidant activity of broccoli after domestic processing. Food Addit Contam 23:1088–1098CrossRefPubMedGoogle Scholar
  13. Granado F, Olmedilla B, Herrero C, Perez-Sacristan B, Blanco I, Blazquez S (2006) Bioavailability of carotenoids and tocopherols from broccoli: in vivo and in vitro assessment. Exp Biol Med 231:1733–1738Google Scholar
  14. Jang MW, Ha BJ (2012) Effects of broccoli on anti-inflammation and anti-oxidation according to extraction solvents. J Food Hyg Safety 27:461–465CrossRefGoogle Scholar
  15. Jeffery EH, Brown AF, Kurilich AC, Keck AS, Matusheski N, Klein BP, Juvik JA (2003) Variation in content of bioactive components in broccoli. J Food Compos Anal 16:323–330CrossRefGoogle Scholar
  16. KATI (2014) http://www.kati.net/kati.doGoogle Scholar
  17. Kaur C, Kumar K, Anil D, Kapoor HC (2007) Variations in antioxidant activity in broccoli (Brassica oleracea L.) cultivars. J Food Biochem 31:621–638CrossRefGoogle Scholar
  18. Kim MS, Lee YS, Kwon HY, Kim JS, Sohn HY (2014) Antioxidative antimicrobial, and anti-proliferative activities of the floret and stalk of broccoli (Brassica oleracea L.). Korean J Microbiol Biotechnol 42:58–66CrossRefGoogle Scholar
  19. Kurilich AC, Jeffery EH, Juvik JA, Wallig MA, Klein BP (2002) Antioxidant capacity of different broccoli (Brassica oleracea) genotypes using the oxygen radical absorbance capacity (ORAC) assay. J Agric Food Chem 50:5053–5057CrossRefPubMedGoogle Scholar
  20. Latte KP, Appel KE, Lampen A (2011) Health benefits and possible risks of broccoli-an overview. Food Chem Toxicol 49:3287–3309CrossRefPubMedGoogle Scholar
  21. Lee JG, Bonnema G, Zhang N, Kwak JH, de Vos RCH, Beekwilder J (2013) Evaluation of glucosinolate variation in a collection of turnip (Brassica rapa) germplasm by the analysis of intact and desulfo glucosinolates. J Agric Food Chem 61:3984–3993CrossRefPubMedGoogle Scholar
  22. Lee JG, Kwak JH, Um YC, Lee SG, Jang YA, Choi CS (2012) Variation of glucosinolate contents among domestic broccoli (Brassica oleracea L. var. italica) accessions. Korean J Hortic Sci Technol 30:743–750CrossRefGoogle Scholar
  23. Lee JJ, Shin HD, Lee YM, Kim AR, Lee MY (2009) Effect of broccoli sprouts on cholesterol-lowering and anti-obesity effects in rats fed high fat diet. J Korean Soc Food Sci Nutr 38:309–318CrossRefGoogle Scholar
  24. Lopez-Cervantes J, Tirado-Noriega LG, Sanchez-Machado DI, Campas-Baypoli ON, Cantu-Soto EU, Nunez-Gastelum JA (2013) Biochemical composition of broccoli seeds and sprouts at different stages of seedling development. Intl J Food Sci Technol 48:2267–2275Google Scholar
  25. Menichini F, Tundis R, Bonesi M, Loizzo MR, Conforti F, Statti G, De Cindio B, Houghton PJ, Menichini F (2009) The influence of fruit ripening on the phytochemical content and biological activity of Capsicum chinense Jacq. Cv Habanero. Food Chem 114:553–560CrossRefGoogle Scholar
  26. MIFAFF (2012) HTTP://LIBRARY.MIFAFF.GO.KR/SKYBLUEIMAGE/7204.PDFGoogle Scholar
  27. Nachshon-Kedmi M, Fares FA, Yannai S (2004) Therapeutic activity of 3,3’-diindolylmethane on prostate cancer in an in vivo model. Prostate 61:153–160CrossRefPubMedGoogle Scholar
  28. Nath A, Bagchi B, Misra LK, Deka BC (2011) Changes in post-harvest phytochemical qualities of broccoli florets during ambient and refrigerated storage. Food Chem 127:1510–1514CrossRefGoogle Scholar
  29. Park MY, Yoon MK, Kwak JH (2014) Antimicrobial and antioxidant activities in different parts and cultivars of broccoli. Korean J Hortic Sci Technol 32:408–414.CrossRefGoogle Scholar
  30. Perez-Balibrea S, Moreno DA, Garcia-Viguera C (2011) Genotypic effects on the phytochemical quality of seeds and sprouts from commercial broccoli cultivars. Food Chem 125:348–354CrossRefGoogle Scholar
  31. Pourcel L, Routaboul JM, Cheynier V, Lepiniec L, Debeaujon I (2006) Flavonoid oxidation in plants: from biochemical properties to physiological functions. Trends Plant Sci 12:29–36CrossRefPubMedGoogle Scholar
  32. Rosa EAS, Rodrigues AS (2001) Total and individual glucosinolate content in 11 broccoli cultivars grown in early and late seasons. HortScience 36:56–59Google Scholar
  33. Sarikamis G, Marquez J, MacCormack R, Bennett RN, Roberts J, Mithen R (2006) High glucosinolate broccoli: a delivery system for sulforaphane. Mol Breeding 18:219–228CrossRefGoogle Scholar
  34. Schreiner MC, Peters PJ, Krumbein AB (2006) Glucosinolates in mixed-packaged mini broccoli and mini cauliflower under modified atmosphere. J Agric Food Chem 54:2218–2222CrossRefPubMedGoogle Scholar
  35. Shofran BG, Purrington ST, Breidt F, Fleming HP (2006) Antimicrobial properties of sinigrin and its hydrolysis products. J Food Sci 63:621–624CrossRefGoogle Scholar
  36. Singleton VL, Rossi Jr JA (1965) Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Amer. J Enol Viticult 16:144–158Google Scholar
  37. Spitz MR, Duphorne CM, Detry MA, Pillow PC, Amos CI, Lei L, de Andrade M, Gu X, Hong WK, Wu X (2000) Dietary intake of isothiocyanates: evidence of a joint effect with glutathione S-transferase polymorphisms in lung cancer risk. Cancer Epidemiol Bio-markers Prev 9:1017–1020Google Scholar
  38. Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Byrne DH (2006) Comparison of ABTS, DPPH, FRAP and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compos Anal 19:669–675CrossRefGoogle Scholar
  39. Traka M, Mithen R (2009) Glucosinolates, isothiocyanates and human health. Phytochem Rev 8:269–282CrossRefGoogle Scholar
  40. Vallejo F, Tomas-Barberan FA, Benavente-Garcia AG, Garcia-Viguera C (2003) Total and individual glucosinolate contents in inflorescences of eight broccoli cultivars grown under various climatic and fertilisation conditions. J Sci Food Agric 83:307–313CrossRefGoogle Scholar
  41. Wang J, Gu H, Yu H, Zhao Z, Sheng X, Zhang X (2012) Genotypic variation of glucosinolates in broccoli (Brassica oleracea var. italica) florets from China. Food Chem 133:735–741CrossRefGoogle Scholar
  42. Williams DJ, Critchley C, Pun S, Nottingham S, O’ Hare TJ (2008) Epithiospecifier protein activity in broccoli: The link between terminal alkenyl glucosinolates and sulphoraphane nitrile. Phytochemistry 69:2765–2773CrossRefPubMedGoogle Scholar
  43. Zhou K, Yu L (2006) Total phenolic contents and antioxidant properties of commonly consumed vegetables grown in Colorado. LWT-Food Sci Technol 39:1155–1162CrossRefGoogle Scholar

Copyright information

© Korean Society for Horticultural Science and Springer-Verlag GmbH 2016

Authors and Affiliations

  • Jung Su Jo
    • 1
  • Shiva Ram Bhandari
    • 1
  • Gwan Ho Kang
    • 2
  • Jun Gu Lee
    • 1
    • 3
  1. 1.Department of Horticulture, College of Agriculture & Life SciencesChonbuk National UniversityJeonjuKorea
  2. 2.Breeding Research Institute, Koregon Co., LtdGimjeKorea
  3. 3.Institute of Agricultural Science & TechnologyChonbuk National UniversityJeonjuKorea

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