Horticulture, Environment, and Biotechnology

, Volume 56, Issue 2, pp 225–232 | Cite as

Characterization of microspore development and pollen tube growth response to self and cross-pollination in Jeju old local citrus species

  • Panha Pok
  • Eun Ui Oh
  • Kyunguk Yi
  • Jong Hoon Kang
  • Boo Young Ko
  • Ho Bang Kim
  • Kwan Jeong SongEmail author
Research Report


This study was conducted to investigate the seed formation potential by the evaluation of microspore development and self- and cross-compatibility through their pollen tube growth response to self and cross pollination in Jeju old local citrus species. Anther and pollen histological analyses were done to observe microspore development for three species including byungkyul, sadoogam, and dangyooza. Pollens of ten citrus species were tested for pollen viability by staining with Lugol’s solution and fluorescein diacetate. Flowers of all species were emasculated and self- and cross-pollinated just before anthesis. Pistils of nine pollinating combinations between the listed three species were collected at 1, 3, 5, 7, and 9 days after pollination for evaluation of a growth pattern of the pollen tube. Pistils of the other combinations were collected at 9 days after pollination for self- and cross-compatibility evaluation. Pistils were prepared by squashing and staining method with aniline blue. Eight pollen developmental stages were recognized and indicated normal development of microspore. These ten citrus species produced high percentage of viable pollens. Pollen tube behavior in compatible and incompatible pollination was distinguished. Pollen tube performance was largely depended on male-female combination. All of these Jeju old local citrus species were male fertile and have the ability to produce enough viable pollens for self- and cross-pollination. All evaluated citrus species were self- and cross-compatible to each other except dangyooza which was self-incompatible.

Additional key words

cross-compatibility Jeju citrus landraces seed formation self-incompatibility 


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Copyright information

© Korean Society for Horticultural Science and Springer-Verlag GmbH 2015

Authors and Affiliations

  • Panha Pok
    • 1
  • Eun Ui Oh
    • 1
  • Kyunguk Yi
    • 1
  • Jong Hoon Kang
    • 2
  • Boo Young Ko
    • 2
  • Ho Bang Kim
    • 3
  • Kwan Jeong Song
    • 1
    • 4
    Email author
  1. 1.Faculty of Bioscience and Industry, SARIJeju National UniversityJejuKorea
  2. 2.Jeju Special Self-governing Province Agricultural Research & Extension ServicesJejuKorea
  3. 3.Life Sciences Research InstituteBiomedic Co.BucheonKorea
  4. 4.Research Institute for Subtropical Agriculture & BiotchnologyJeju National UniversityJejuKorea

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