Comparison of accumulation of stilbene compounds and stilbene related gene expression in two grape berries irradiated with different light sources
- 214 Downloads
In this study, the accumulation of stilbene compounds and the expression of genes related to their syntheses in ‘Campbell Early’ and ‘Kyoho’ grapes (Vitis sp.) were investigated by irradiating the harvested berries with four different light sources (white fluorescent and purple, blue, and red LED lights) for 48 hours. The total concentrations of five stilbene derivatives at 24 hours after irradiation differed in response to different light sources and cultivars. The accumulation of stilbenic compounds in the skins of two grape cultivars and the expression of PAL and STS1 genes were mainly induced under red and blue LED lights. The expression of PAL, CHS, CHI, STS1, STS12, and ROMT was differently induced in response to irradiation with different light sources in both grape cultivars. The mRNA levels of PAL and STS1 were higher than those of CHS, CHI, STS12 and ROMT in the two grapes berries. Overall, the results of this study indicated that red and blue LED induced the accumulation of stilbene compounds and the expression of genes related to their syntheses in grape berries, suggesting that irradiation with LEDs can be used to induce the accumulation of phytochemicals that have positive impacts on human health via the induction of related genes.
Additional key wordsLED phytochemical red light resveratrol Vitis sp.
Unable to display preview. Download preview PDF.
- Ahn, S.Y., S.A. Kim, J.H. Han, S.J. Choi, and H.K. Yun. 2013. Induction of defense-related responses and suppression of grey mold in grapevines treated with defense response signaling molecules. J. Amer. Pomol. Soc. 67:104–116.Google Scholar
- Choi, S.J. 2011. The identification of stilbene compounds and the change of their contents in UV-irradiated grapevine leaves. Kor. J. Hort. Sci. Technol. 29:374–381.Google Scholar
- Choi, S.J. 2012. The influence of UV irradiation on stilbene contents and gray mold incidence in grapevine leaves. Kor. J. Hort. Sci. Technol. 30:493–500.Google Scholar
- Ebisawa, M., K. Shoji, M. Kato, K. Shimomura, F. Goto, and T. Yoshihara. 2008. Supplementary ultraviolet radiation B together with blue light at night increased quercetin content and flavonol synthase gene expression in leaf lettuce (Lactuca sativa L.). Environ. Control Biol. 46:1–11.CrossRefGoogle Scholar
- González-Barrio, R., D. Beltrán, E. Cantos, M.I. Gil, J.C. Espín, and F.A. Tomás-Barberán. 2006. Comparison of ozone and UV-C treatments on the postharvest stilbenoid monomer, dimer, and trimer induction in var. ‘Superior’ white table grapes. J. Agric. Food Chem. 54:4222–4228.CrossRefPubMedGoogle Scholar
- Jeandet, P., R. Bessis, and B. Gautheron. 1991. The production of resveratrol (3,5,4′-trihydroxystilbene) by grape berries in different developmental stages. Amer. J. Enol. Vitic. 42:41–46.Google Scholar
- Johkan, M., K. Shoji, F. Goto, S. Hashida, and T. Yoshihara. 2010. Blue light-emitting diode light irradiation of seedlings improves seedling quality and growth after transplanting in red leaf lettuce. HortScience 45:1809–1814.Google Scholar
- Schmidlin, L., A. Poutaraud, P. Claudel, P. Mestre, E. Prado, M. Santos-Rosa, S. Wiedemann-Merdinoglu, F. Karst, D. Merdinoglu, and P. Hugueney. 2008. A stress-inducible resveratrol o-methyltransferase involved in the biosynthesis of pterostilbene in grapevine. Plant Physiol. 148:1630–1639.CrossRefPubMedCentralPubMedGoogle Scholar
- Tříska, J. and M. Houška. 2012. Physical methods of resveratrol induction in grapes and grape products —A review. Czech J. Food Sci. 30:489–502.Google Scholar
- Tuan, P.A., A.A. Thwe, Y.B. Kim, J.K. Kim, S.-J. Kim, S. Lee, S.-O. Chung, and S.U. Park. 2013. Effects of white, blue, and red light-emitting diodes on carotenoid biosynthetic gene expression levels and carotenoid accumulation in sprouts of tartary buckwheat (Fagopyrum tataricum Gaertn.). J. Agric. Food Chem. 61:12356–12361.CrossRefPubMedGoogle Scholar
- Wang, W., K. Tang, H.-R. Yang, P.-F. Wen, P. Zhang, H.-L. Wang, and W.-D. Huang. 2010. Distribution of resveratrol and stilbene synthase in young grape plant (Vitis vinifera L. cv. Cabernet Sauvignon) and the effect of UV-C on its accumulation. Plant Physiol. Bioch. 48:142–152.CrossRefGoogle Scholar
- Wang, Y., B. Zhou, M. Sun, Y. Li, and S. Kawabata. 2012b. UV-A light induces anthocyanin biosynthesis in a manner distinct from synergistic blue + UV-B light and UV-A/blue light responses in different parts of the hypocotyls in turnip seedlings. Plant Cell Physiol. 53:1470–1480.CrossRefPubMedGoogle Scholar