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Horticulture, Environment, and Biotechnology

, Volume 54, Issue 6, pp 475–483 | Cite as

Water deficit affects plant and soil water status, plant growth, and ginsenoside contents in American ginseng

  • Jinwook Lee
  • Kenneth W. Mudge
Research Report Cultivation Physiology

Abstract

American ginseng (Panax quinquefolius L.) produces pharmacologically active secondary compounds known as ginsenosides which have been shown to be influenced by both genetic and environmental factors. In a greenhouse experiment, effects of water deficit on ginseng plant growth, predawn leaf water potential (ΨLeaf), soil water potential (ΨSoil), leaf abscisic acid (ABA) concentration, and root ginsenoside contents as well as photosynthesis-related physiological responses were studied. Three-year-old seedlings, grown in 200 mL volume of plastic pots, were well watered for 45 days prior to the initiation of water deficit treatments. Plants in the water deficit treatments were irrigated every 10 or 20 days for the mild and severe water deficit treatments, respectively, while the control plants were watered every 4 days. The experiment was terminated after 15, 6, and 3 dry down cycles (60 days) for the control, mild, and severe water deficit treatments, respectively. As water deficit progressed, both ΨSoil and ΨLeaf decreased, but foliar ABA concentration increased. Other physiological responses to water deficit, including transpiration rate, stomatal conductance, and CO2 assimilation rate, were decreased. Water deficit decreased root growth, but unaffected shoot growth. Foliar chlorophyll content was also decreased in the water deficit treatments. The contents of individual ginsenosides Re, Rb1, Rc and Rd, and total ginsenosides were increased in the storage roots of water deficit-treated plants as compared with well-watered controls. Rootlet fresh weight before transplanting (RFWBT) as a covariate had a significant effect on the contents of ginsenoside Rb1, Rc, and Rb2. Overall, the results indicate that water deficit could contribute not only to reducing plant performance but also increasing the levels of ABA and certain ginsenoisdes.

Additional key words

abscisic acid (ABA) drought stress evapotranspiration leaf water potential Panax quinquefolium L. soil water potential 

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Literature Cited

  1. Alves, A.A.C. and T.L. Setter. 2000. Response of cassava to water deficit; leaf area growth and abscisic acid. Crop Sci. 40:131–137.CrossRefGoogle Scholar
  2. Arnon, D.I. 1949. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 24:1–15.PubMedCentralPubMedCrossRefGoogle Scholar
  3. Assinewe, V., B.R. Baum, D. Gagnon, and J.T. Arnason. 2003. Phytochemistry of wild populations of Panax quinquefolius L. (North American ginseng). J. Agric. Food Chem. 51:4549–4553.PubMedCrossRefGoogle Scholar
  4. Attele, A.S., J.A. Wua, and C. S. Yuan. 1999. Ginseng pharmacology: Multiple constituents and multiple actions. Biochem. Pharmacol. 58:1685–1693.PubMedCrossRefGoogle Scholar
  5. Barbara, K., K. Ewa, K. Jerzy, and C. Aleksander. 2006. The effect of growth regulators on quality parameters and ginsenosides accumulation in Panax quinquefolium L. roots. Plant Growth Regulat. 48:13–19.CrossRefGoogle Scholar
  6. Bauerle, W.L., W.W. Inman, and J.B. Dudley. 2006. Leaf abscisic acid accumulation in response to substrate water content: Linking leaf gas exchange regulation with leaf abscisic acid concentration. J. Amer. Soc. Hort. Sci. 131:295–301.Google Scholar
  7. Bauerle, W.L., T.H. Whitlow, T.L. Setter, and F.M. Vermeylen. 2004. Abscisic acid synthesis in Acer rubrum L. leaves — A vapor-pressure-deficit-mediated response. J. Amer. Soc. Hort. Sci. 129:182–187.Google Scholar
  8. Beyfuss, R.L. 1999. American ginseng production in woodlots. Agroforest. Notes 14:1–4.Google Scholar
  9. Bouchereau, A., N. Clossais-Besnard, A. Bensaoud, L. Leport, and M. Renard. 1996. Water stress effects on rapeseed quality. Eur. J. Agron. 5:19–30.CrossRefGoogle Scholar
  10. Clemente, H.S. and T.E. Marler. 1996. Drought stress influences gas-exchange responses of papaya leaves to rapid changes in irradiance. J. Amer. Soc. Hort. Sci. 121:292–295.Google Scholar
  11. Delfine, S., R. Tognetti, F. Loreto, and A. Alvino. 2002. Physiological and growth responses to water stress in field-grown bell pepper (Capsicum annuum L.). J. Hort. Sci. Biotechnol. 77:697–704.Google Scholar
  12. El-Sharkawy, M.A., A.H. Del Pilar, and C. Hershey. 1992. Yield stability of cassava during prolonged mid-season water stress. Exp. Agric. 28:165–174.CrossRefGoogle Scholar
  13. Ennahli, S. and H.J. Earl. 2005. Physiological limitations to photosynthetic carbon assimilation in cotton under water stress. Crop Sci. 45:2374–2382.CrossRefGoogle Scholar
  14. Fournier, A.R., J.T.A. Proctor, L. Gauthier, S. Khanizadeh, A. Belanger, A. Gosselin, and M. Dorais. 2003. Understory light and root ginsenosides in forest-grown Panax quinquefolius. Phytochemistry 63:777–782.PubMedCrossRefGoogle Scholar
  15. Jochum, G.M., K.W. Mudge, and R.B. Thomas. 2007. Elevated temperatures increase leaf senescence and root secondary metabolite concentrations in the understory herb Panax quinquefolius (Araliaceae). Amer. J. Bot. 94:819–826.CrossRefGoogle Scholar
  16. Kirakosyan, A., E. Seymour, P.B. Kaufman, S. Warber, S. Bolling, and S.C. Chang. 2003. Antioxidant capacity of polyphenolic extracts from leaves of Crataegus laevigata and Crataegus monogyna (Hawthorn) subjected to drought and cold stress. J. Agric. Food Chem. 51:3973–3976.PubMedCrossRefGoogle Scholar
  17. Konsler, T.R., S.W. Zito, J.E. Shelton, and E.J. Staba. 1990. Lime and phosphorus effects on American ginseng. II. Root and leaf ginsenoside content and their relationship. J. Amer. Soc. Hort. Sci. 115:575–580.Google Scholar
  18. Lee, J. and K.W. Mudge. 2013. Gypsum effects on plant growth, nutrients, ginsenosides, and their relationship in American ginseng. Hort. Environ. Biotechnol. 54:228–235.CrossRefGoogle Scholar
  19. Lee, S.S., D.C. Yang, and Y.T. Kim. 1982. Effects of soil water regimes on photosynthesis, growth and development of ginseng plant (Panax ginseng C.A. Meyer). Kor. J. Crop Sci. 27:175–181.Google Scholar
  20. Li, T.S.C. 1995. Asian and American ginseng — A review. Hort-Technology 5:27–34.Google Scholar
  21. Li, T.S.C. and R.G. Berard. 1998. Effects of soil moisture on the growth of American ginseng (Panax quinquefolium L.). J. Ginseng Res. 22:122–125.Google Scholar
  22. Li, T.S.C. and G. Mazza. 1999. Correlations between leaf and soil mineral concentrations and ginsenoside contents in American ginseng. HortScience 34:85–87.Google Scholar
  23. Lim, W., K.W. Mudge, and J.W. Lee. 2006. Effect of water stress on ginsenoside production and growth of American ginseng. HortTechnology 16:517–522.Google Scholar
  24. Lim, W., K.W. Mudge, and F. Vermeylen. 2005. Effects of population, age, and cultivation methods on ginsenoside content of American ginseng (Panax quinquefolium). J. Agric. Food Chem. 53:8498–8505.PubMedCrossRefGoogle Scholar
  25. Liu, Z. 2000. Drought-induced in vivo synthesis of camptothecin in Camptotheca acuminata seedlings. Physiol. Plant. 110:483–488.Google Scholar
  26. Mork, S.K., S.Y. Son, and H. Park. 1981. Root and top growth of Panax ginseng at various soil moisture regime. Kor. J. Crop Sci. 26:115–120.Google Scholar
  27. Proctor, J.T.A. and W.G. Bailey. 1987. Ginseng: Industry, botany, and culture. Hort. Rev. 9:187–236.Google Scholar
  28. Reaves, M.E., 2003. Gas exchange and water relations of red maple Acer rubrum L. ecotypes and cultivars in response to drought. Cornell University, Ithaca, NY.Google Scholar
  29. Setter, T.L., B.A. Flannigan, and J. Melkonian. 2001. Loss of kernel set due to water deficit and shade in maize: Carbohydrate supplies, abscisic acid, and cytokinins. Crop Sci. 41:1530–1540.CrossRefGoogle Scholar
  30. Trejo, C.L., A.L. Clephan, and W.J. Davies. 1995. How do stomata read abscisic acid signals? Plant Physiol. 109:803–811.PubMedCentralPubMedGoogle Scholar
  31. Wang, Z., B. Huang, S.A. Bonos, and W.A. Meyer. 2004. Abscisic acid accumulation in relation to drought tolerance in Kentucky bluegrass. HortScience 39:1133–1137.Google Scholar
  32. Zhang, J. and W.J. Davies. 1990. Changes in the concentration of ABA in xylem sap as a function of changing soil water status can account for changes in leaf conductance and growth. Plant Cell Environ. 13:277–285.CrossRefGoogle Scholar
  33. Zhang, Y.J., Z.K. Xie, Y.J. Wang, P.X. Su, L.P. An, and H. Gao. 2011. Effect of water stress on leaf photosynthesis, chlorophyll content, and growth of oriental lily. Russian J. Plant Physiol. 58:844–850.CrossRefGoogle Scholar

Copyright information

© Korean Society for Horticultural Science 2013

Authors and Affiliations

  1. 1.Department of HorticultureCornell UniversityIthacaUSA
  2. 2.USDA-ARSTree Fruit Research LaboratoryWenatcheeUSA

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