Horticulture, Environment, and Biotechnology

, Volume 53, Issue 6, pp 505–512 | Cite as

Antioxidant enzyme activity and chilling injury during low-temperature storage of Kiwifruit cv. Hongyang exposed to gradual postharvest cooling

  • Qingzhen Yang
  • Jingping RaoEmail author
  • Shunchao Yi
  • Kun Meng
  • Jianfeng Wu
  • Yali Hou
Research Report Postharvest Technology


Kiwifruits (Actinidia chinensis cv. Hongyang) were treated by direct cooling and gradual cooling to investigate the effect of cooling treatment on chilling injury. The direct cooling fruits were immediately cooled at 0°C after harvest. The gradual cooling fruits were held for 3 days at 5 °C (from 5 °C to 0°C), or for 7 days at 2°C (from 2°C to 0°C), or decreased in temperature from 15°C to 5°C by 5°C at 1 days intervals and then maintained at 5°C for 3 days plus a subsequent period of of 7 days at 2 (from 15°C to 0°C). After the above treatments, then those fruit were stored at 0 ± 0.5°C, 90% to 95% RH for 80 days. Gradual cooling (from 15°C to 0°C) significantly maintained higher percentage of accepted fruit and lower chilling injury index and chilling injury incidence of fruit compared with the direct cooling. Some attributes were then assayed in the fruits treated with gradual cooling (from 15°C to 0°C). Gradual cooling (from 15°C to 0°C) inhibited increases in membrane permeability, malondialdehyde content, superoxide anion production rate, and H2O2 content. At the same time, fruit cooled gradually (from 15°C to 0°C) exhibited higher superoxide dismutase, catalase, ascorbate peroxidase, and peroxidase activities than those treated by direct cooling during storage. The present study indicated that enhancement in antioxidant enzyme activity may be attributed to the reduction in CI symptoms by the gradual cooling treatment (from 15°C to 0°C).

Additional key words

cell membranes chilling tolerance cold acclimation lipid peroxidation reactive oxygen species 


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Literature cited

  1. Aebi, H. 1984. Catalase in vitro. Methods Enzymol. 105:121–126.PubMedCrossRefGoogle Scholar
  2. Antunes, M.D.C. and E.M. Sfakiotakis. 2002. Chilling induced ethylene biosynthesis in ‘Hayward’ kiwifruit following storage. Sci. Hortic. 92:29–39.CrossRefGoogle Scholar
  3. Antunes, M.D.C. and E.M. Sfakiotakis. 2008. Changes in fatty acid composition and electrolyte leakage of ‘Hayward’ kiwifruit during storage at different temperatures. Food Chem. 110:891–896.CrossRefGoogle Scholar
  4. Ariel, R.V., A.M. Gustavo, R.C. Alicia, and M.C. Pedro. 2006. Effect of heat treatment on strawberry fruit damage and oxidative metabolism during storage. Postharvest Biol. Technol. 40:116–122.CrossRefGoogle Scholar
  5. Arpaia, M.L., A.A. MIitchell, A.A. Kader, and G. Mayer. 1985. Effects of 2% and varying concentrations of with or without on the storage performance of kiwifruit. J. Am. Soc. Hortic. Sci. 110:200–203.Google Scholar
  6. Aurelio, B.G., V.G. Misael, C.C. José, C.L. Armando, and A.V. José. 2010. Effect of gradual cooling storage on chilling injury and phenylalanine ammonia-lyase activity in tomato fruit. J. Food Biochem. 34:295–307.CrossRefGoogle Scholar
  7. Burdon, J., N. Lallu, K. Francis, and H. Boldingh. 2007. The susceptibility of kiwifruit to low temperature breakdownis associated with pre-harvest temperatures and at-harvestsoluble solids content. Postharvest Biol. Technol. 43:283–290.CrossRefGoogle Scholar
  8. Cao, S.F., Y.H. Zheng, K.T. Wang, P. Jin, and H.J. Rui. 2009. Methyl jasmonate reduces chilling injury and enhances antioxidant enzyme activity in postharvest loquat fruit. Food Chem. 115:1458–1463.CrossRefGoogle Scholar
  9. Cao, S.F., Z.F. Yang, Y.T Cai, and Y.T Zheng. 2011. Fatty acid composition and antioxidant system in relation to susceptibility of loquat fruit to chilling injury. Food Chem. 127:1777–1783.CrossRefGoogle Scholar
  10. Dhindsa, R.S., P.P. Dhindsa, and T.A. Thorpe. 1981. Leaf senescence: Correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J. Exp. Bot. 32:93–101.CrossRefGoogle Scholar
  11. Gerasopoulos, D. and P.D. Drogoudi. 2005. Summer-pruning and preharvest calcium chloride sprays affect storability and low temperature breakdown incidence in kiwifruit. Postharvest Biol. Technol. 36:303–308.CrossRefGoogle Scholar
  12. Hammerschmidt, R., E.M. Nuckles, and J. Kuc. 1982. Ligni cation as amechanismfor induced systemic resistance in cucumber. Physiol. Plant Pathol. Physiol. 20:61–71.CrossRefGoogle Scholar
  13. Hariyadi, P. and L.P. Kirk. 1991. Chilling-induced oxidative stress in cucumber fruits. Postharvest Biol. Technol. 1:33–45.CrossRefGoogle Scholar
  14. Hodges, D.M., G.E. Lester, K.D. Munro, and P.M. Toivonen. 2004. Oxidative stress: Importance for postharvest quality. HortScience 39:924–929.Google Scholar
  15. Imahori, Y., M. Takemura, and J.H. Bai. 2008. Chilling-induced oxidative stress and antioxidant responses in mume (Prunus mume) fruit during low temperature storage. Postharvest Biol. Technol. 49:54–60.CrossRefGoogle Scholar
  16. Jiang, Y.M. and F. Chen. 1995. A study on polyamine change and browning of fruit during cold storage of litchi fruit. Postharvest Biol. Technol. 5:245–250.CrossRefGoogle Scholar
  17. Lallu, N. 1997. Low temperature breakdown in kiwifruit. Acta Hortic. 444:579–585.Google Scholar
  18. Lim, B.S., J.K. Kim, K.C. Gross, Y.S. Hwang, and J.H. Kim. 2005. Gradual postharvest cooling reduces blackening disorder in ‘Niitaka’ pear (Pyrus pyrifolia) fruits. J. Kor. Soc. Hort. Sci. 46:311–316.Google Scholar
  19. Lurie, S. and A. Sabehat. 1997. Prestorage temperature manipulations to reduce chilling injury in tomatoes. Postharvest Biol. Technol. 11:57–62.CrossRefGoogle Scholar
  20. Nakano, Y. and K. Asada. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 22:867–880.Google Scholar
  21. Patterson, B.D., E.A. Macrae, and I. B. Ferguson. 1984. Estimation of hydrogen peroxide in plant extracts using titanium (IV). Anal Biochem. 139:487–492.PubMedCrossRefGoogle Scholar
  22. Sala, J.M. 1998. Involvement of oxidative stress in chilling injury in cold-stored mandarin fruits. Postharvest Biol. Technol. 13:255–261.CrossRefGoogle Scholar
  23. Sfakiotakis, E.M., G. Chlioumis, and D. Gerasopoulos. 2005. Preharvest chilling reduces low temperature breakdown incidence of kiwifruit. Postharvest Biol. Technol. 38:169–174.CrossRefGoogle Scholar
  24. Song, L.L., H.Y. Gao, H.J. Chen, J.L. Mao, Y.J. Zhou, W.X. Chen, and Y.M. Jiang. 2009. Effects of short-term anoxic treatment on antioxidant ability and membrane integrity of postharvest kiwifruit during storage. Food Chem. 114:1216–1221.CrossRefGoogle Scholar
  25. Wang, C.Y. 1982. Physiological and biochemical responses of plants to chilling stress. HortScience 17:173–186.Google Scholar
  26. Wang, A.G. and G.H. Luo. 1990. Quantitative relation between the reaction of hydroxylamine and superoxide anion radicals in plants. Plant Physiol. Commun. 26:55–57.Google Scholar
  27. Wang, C.Y. 1993. Approaches to reduce chilling injury of fruits and vegetables. Hort. Rev. 15:63–95.Google Scholar
  28. Wu, F.H., H.Q. Yang, Y.Z. Chang, J.Y. Cheng, S.F.X. Bai, and J.Y. Yin. 2012. Effects of nitric oxide on reactive oxygen species and antioxidant capacity in chinese bayberry during storage. Scientia Hor. 135:106–111.CrossRefGoogle Scholar
  29. Xiong, X.M., J.P. Rao, S.Q. Dai, and Q.Z. Fang. 2006. Effect of cold shock treatment on the quality and anti-oxidative enzyme activities of nectarine fruits during storage. Acta Bot. Boreal-Occident. Sin. 26:473–477.Google Scholar
  30. Yang, A.P., S.F. Cao, Z.F. Yang, Z.T. Cai, and Z.H. Zheng. 2011a. γ-aminobutyric acid treatment reduces chilling injury and activates the defence response of peach fruit. Food Chem. 129:1619–1622.CrossRefGoogle Scholar
  31. Yang, H.Q., F.H. Wu, and J. Y. Cheng. 2011b. Reduced chilling injury in cucumber by nitric oxide and the antioxidant response. Food Chem. 127:1237–1242.CrossRefGoogle Scholar
  32. Zhao, Y.L., J.H. Li, J. F. Shi, X.Y. Zhang, L. Wang, and H.R. Wang. 2009. Effects of slow cooling treatment on chilling injury and physiological changes of pomegranate fruit during cold storage. Zhongguo Nong Xue Tong Bao. 25:102–105.Google Scholar
  33. Zheng, Y.H., R.W.M. Fung, S.Y. Wang, and C.Y. Wang. 2008. Transcript levels of antioxidative genes and oxygen radical scavenging enzyme activities in chilled zucchini squash in response to superatmospheric oxygen. Postharvest Biol. Technol. 47:151–158.CrossRefGoogle Scholar
  34. Zhu, S.H., L.N. Sun, M.C. Liu, and J. Zhou. 2008. Effect of nitric oxide on reactive oxygen species and antioxidant enzymes in kiwifruit during storage. J. Sci. Food Agric. 88:2324–2331.CrossRefGoogle Scholar

Copyright information

© Korean Society for Horticultural Science 2012

Authors and Affiliations

  • Qingzhen Yang
    • 1
    • 2
  • Jingping Rao
    • 1
    Email author
  • Shunchao Yi
    • 1
  • Kun Meng
    • 1
  • Jianfeng Wu
    • 1
  • Yali Hou
    • 1
  1. 1.College of HorticultureNorthwest A&F UniversityYangling, Shannxi ProvincePR China
  2. 2.Department of Life SciencesYuncheng UniversityYuncheng, Shanxi ProvincePR China

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