Pollen allergic risk assessment of genetically modified virus resistant pepper and functional Chinese cabbage
- 346 Downloads
Pollen of genetically modified (GM) pepper containing the gene for cucumber mosaic virus (CMV) coat protein (CP) and GM Chinese cabbage with high phenylethylisothiocyanate (PEITC) content was investigated for assessment of allergic risk. Amino acid (AA) sequences of the inserted gene products of GM virus resistant pepper and GM Chinese cabbage with high PEITC content were compared with those of known allergens. No known allergen greater than 35% AA sequence homology, over 80 AA window or more than 8 consecutive identical AA was found. Protein patterns of GM/non-GM pepper and Chinese cabbage pollen extracts in SDS-PAGE analysis showed the same distribution of protein bands among the GM and non-GM pepper or Chinese cabbage, respectively. Sera from pollen allergic patients showed some IgE reactivity via immunoblotting and ELISA; however, no differences were observed between the pollen of GM and non-GM pepper or Chinese cabbage, respectively. Based on these results, we conclude that pollens of the virus resistant GM pepper and GM Chinese cabbage with high PEITC have no differences in their protein composition or allergenicity relative to non-GM pepper and Chinese cabbage.
Additional key wordsallergy cucumber mosaic virus (CMV) GM phenylethylisothiocyanate (PEITC)
Unable to display preview. Download preview PDF.
- Bang, S.N., Y.S. Jung, S.J. Eom, G.B. Kim, K.H. Chung, G.P. Lee, D.Y. Son, K.W. Park, J.S. Hong, K.H. Ryu, and C. Lee. 2012. Assessment of the cucumber mosaic virus coat protein by expression evaluation in a genetically modified pepper and Escherichia coli BL21. J. Food Biochem. (in press).Google Scholar
- Bruce, M.C. 2002. Food safety evaluation of crops produced through biotechnology. J. Amer. Coll. Nutr. 21:166–173.Google Scholar
- Codex. 2009. Foods derived from modern biotechnology. 2nd ed. Codex Alimentarius Commission, Rome, Italy. ftp://ftp.fao.org/codex/Publications/Booklets/Biotech/Biotech_2009e.pdf.Google Scholar
- Flavell, R.B., E. Dart, R.L. Fuchs, and R.T. Fraley. 1992. Selectable marker genes: Safe for plants? Bio. Technol. 10:141–144.Google Scholar
- Fuchs, R.L., J.E. Ream, B.G. Hammond, M.W. Naylor, R.M. Leimgruber, and S.A. Berberich. 1993. Safety assessment of the neomycin phosphotransferase II (npt-II) protein. Bio. Technol. 11:1543–1547.Google Scholar
- Goldstein, D.A., B. Tinland, L.A. Gilbertson, J.M. Staub, G.A. Bannon, R.E. Goodman, R.L. McCoy, and A. Silvanovich. 2005. Human safety and genetically modified plants: a review of antibiotic resistance markers and future transformation selection technologies. J. Appl. Microbiol. 99:7–23.PubMedCrossRefGoogle Scholar
- James, C. 2009. Executive summary of global status of commercialized Biotech/GM crops: 2009. ISAAA Briefs. 2009, No. 41. ISAAA: Ithaca, NY, USAGoogle Scholar
- Kim, H.S., S.H. Kim, Y.D. Park. 2003. Development of rescue cloning vector with phosphinothricin resistant gene for effective T-DNA tagging. Kor. J. Hort. Sci. 44:407–411.Google Scholar
- Lee, M.Y., J.H. Lee, H.I. Ahn, M.J. Kim, N.H. Her, J.K. Choi, C.H. Harn, and K.H. Ryu. 2006. Identification and sequence analysis of RNA3 of a resistance-breaking cucumber mosaic virus isolate on Capsicum annuum. Plant Pathol. J. 23:265–270.Google Scholar
- Mittag D., J. Akkerdaas, B.K. Ballmer-Weber, L. Vogel, M. Wensing, W.M. Becker, S.J. Koppelman, A.C. Knulst, A. Helbling, S.L. Hefle, R. Van Ree, and S. Vieths. 2004. Ara h 8, a Bet v 1-homologous allergen from peanut, is a major allergen in patients with combined birch pollen and peanut allergy. J. Allergy Clin. Immunol. 114:1410–1417.PubMedCrossRefGoogle Scholar
- Tawfiq, N., R.K. Heaney, J.A. Plumb, G.R. Fenwick, S.R. Musk, and G. Williamson. 1995. Dietary glucosinolates as blocking agents against carcinogenesis-breakdown products assessed by induction of quinone reductase activity in murine hepal 1c1c7 cells. Carcinogenesis 16:1191–1194.PubMedCrossRefGoogle Scholar