In vitro propagation of myco-heterotrophic Gastrodia elata
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Gastrodia elata must establish symbiotic associations with different compatible fungi at seed germination and during vegetative growth. One of the major problems associated with its field production is the degeneration of tubers, which is mainly caused by multiple regeneration via vegetative propagation using immature tubers. To overcome this problem, an in vitro asexual propagation method via artificial pollination was developed. Seeds were first germinated on medium containing a leaf-disc from four different Quercus species, previously infected with Mycena osmundicola. Among the four species tested, Q. acutissima was determined to be the best organic resource and the germination rate reached up to 40.4% when a leaf-disc (2 × 2 cm) was provided. Subsequent infection with Armillaria mellea allowed the protocorms to further develop into immature tubers, which can be directly used for field production. In this study, we established an in vitro symbiotic propagation system of G. elata, which might be an efficient way to prevent degeneration of G. elata tubers and the reduction of yield in the field.
Additional key wordsachlorophyllous orchid Armillaria melle Mycena osmundicola symbiotic propagation
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- Chang, H.M. and P.H. But. 1986. Pharmacology and application of Chinese materia medica, Vol. 1. World Scientific, Singapore.Google Scholar
- Huang, Z.L. 1985. Pharmacologic studies and clinical applications of Gastrodia elata Bl. J. Modern Develop. Trad. Media 5:251–254.Google Scholar
- Kikuchi, G., M. Higuchi, H. Yoshimura, T. Morota, and A. Suzuki. 2008. In vitro symbiosis between Gastrodia elata Blume (Orchidaceae) and Armillaria Kummer (Tricholomataceae) species isolated from the orchid tuber. J. Jap. Bot. 83:77–87.Google Scholar
- Kikuchi, G., M. Higuchi, T. Morota, E. Nagasawa, and A. Suzuki. 2008. Fungal symbiont and cultivation test of Gastrodia elata Blume (Orchidaceae). J. Jap. Bot. 83:88–95.Google Scholar
- Kusano, S. 1911. Gastrodia elata and its symbiotic association with Armillaria mellea. Imperial Univ. Tokyo J. College Agr. 4:1–65.Google Scholar
- Leake, J.R. 2005. Plants parasitic on fungi: unearthing the fungi in myco-heterotrophys and debunking the ’saprophytic’ plant myth. Mycol. 19:113–122.Google Scholar
- Smith, S.E. and D.J. Read. 1997. Mycorrhizal symbiosis. 2nd ed. Academic Press, San Diego.Google Scholar
- Xu, J.T. and S.X. Guo. 1991. The relationship of nutrient between Gastrodia elata — Mycena osmundicola and Armillaria mella and its application in the cultivation. J. Med. Res. 1:31–32.Google Scholar
- Zou, N., X. Bai, J. Lv, J. Yang, G. Xu, and D. Sun. 2010. Study on symbiotic mechanism between Gastrodia elata Blume and Armillaria mellea in tissue culture system. Medicinal Plant 1:91–92.Google Scholar