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Karyotype analysis of Lilium tigrinum by FISH

  • Yoon-Jung Hwang
  • Hyun Hee Kim
  • Jong-Bo Kim
  • Ki-Byung Lim
Research Report

Abstract

The detailed karyotypes of diploid (2n = 2x = 24) and triploid (2n = 3x = 36) of L. tigrinum were constructed based on fluorescence in situ hybridization (FISH) using 5S rDNA and 45S rDNA probes. The lengths of mitotic metaphase chromosomes ranged from 15.92 to 30.18 um, with a total length/genome of 250.46 um in diploid, and 15.24 to 28.16 um, with a total length/genome of 233.42 um in triploid. The chromosome composed of two pairs of metacentrics (Chromosomes 1 and 2), four pairs of subtelocentrics (Chromosomes 3, 4, 5, and 6) and six pairs of telocentrics (Chromosomes 7, 8, 9, 10, 11, and 12) in diploid L. tigrinum, two pairs of metacentrics (Chromosomes 1 and 2), six pairs of subtelocentrics (Chromosomes 3, 4, 5, 6, 7, and 11) and four pairs of telocentrics (Chromosomes 8, 9, 10, and 12) in triploid L. tigrinum. Ten (Chromosomes 1, 2, 6, 7, and 11) or twelve (Chromosomes 1, 2, 4, 6, 7, and 11) loci of 45S rDNA were detected in the diploid chromosomes whereas fifteen loci were detected in triploid chromosomes (Chromosomes 1, 2, 6, 7, and 11). Two loci of 5S rDNAs were located close to each other at the both diploid and triploid chromosome 3. New cytotypes of secondary constriction were observed in diploid (1-1-1-0) and triploid (3-2-1-1).

Additional key words

chromosome morphology cytotype lily species rDNA secondary constriction 

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Copyright information

© Korean Society for Horticultural Science 2011

Authors and Affiliations

  • Yoon-Jung Hwang
    • 1
  • Hyun Hee Kim
    • 2
  • Jong-Bo Kim
    • 3
  • Ki-Byung Lim
    • 1
  1. 1.Department of Horticultural ScienceKyungpook National UniversityDaeguKorea
  2. 2.Department of Life Science, Plant Biotechnology InstituteSahmyook UniversitySeoulKorea
  3. 3.Department of Biotechnology, College of Biomedical & Health SciencesKonkuk UniversityChoongjuKorea

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