Simultaneous determination of luteolin and luteoloside in dandelions using HPLC
The content of the two flavonoids, luteolin and luteoloside, in dandelions was determined by high-performance liquid chromatography. A Nucleosil®100-5C18 column was used for the determination of luteolin and luteoloside concentrations. The mobile phase was a mixture of acetonitrile and water containing 0.1% formic acid (99.9:0.1, v/v). The gradient elution program was 10% to 23% acetonitrile within 60 min at a flow rate of 1.0 mL·min−1. The ultraviolet spectra were recorded at 350 nm for the identification of the two flavonoids. The contents of luteolin and luteoloside were highest in T. officinale flowers (5.817 ± 0.030 mg·g−1) and in T. coreanum leaves (6.205 ± 0.087 mg·g−1), respectively. The total content of luteolin and luteoloside in T. coreanum was about 1.3–2 times higher than that found in T. ohwianum and T. officinale.
Additional key wordsisolation phytochemical quantitative analysis structure determination
- Khan, I.A. and E.A. Abourashed. 2010. Leung’s Encyclopedia of Common Natural Ingredients. Used in Food, Drugs and Cosmetics, John Wiley & Sons, New Jersey, U.S.A.Google Scholar
- Lee, H.H. and S.Y. Lee. 2008. Cytotoxic and antioxidant effects of Taraxacum coreanum Nakai and T. officinale WEB. extracts. Korean J. Medicinal Crop Sci. 16:79–85.Google Scholar
- Markham, K.R., V.M. Chari., and T.J. Mabry. 1982. Carbon-13 NMR spectroscopy of flavonoids. In “The flavonoids: Advances in research”, Chapman & Hall, London, England.Google Scholar
- Tita, B., U. Bello, P. Faccendini, R. Bartolini, and P. Bolle. 1993. Taraxacum officinale W.: Pharmacological effect of ethanol extract. Pharmacol. Res. 27:23–24.Google Scholar
- Wolbis, M., M. Królikowska, and P. Bednarek. 1993. Polyphenolic compounds in Taraxacum officinale. Acta Pol. Pharm.-Drug Res. 50:153–158.Google Scholar