Simultaneous determination of luteolin and luteoloside in dandelions using HPLC

  • Sullim Lee
  • Saem Han
  • Hye Min Kim
  • Jeong Min Lee
  • Jongkee Kim
  • Chun-Geon Park
  • Sanghyun Lee
Research Report

Abstract

The content of the two flavonoids, luteolin and luteoloside, in dandelions was determined by high-performance liquid chromatography. A Nucleosil®100-5C18 column was used for the determination of luteolin and luteoloside concentrations. The mobile phase was a mixture of acetonitrile and water containing 0.1% formic acid (99.9:0.1, v/v). The gradient elution program was 10% to 23% acetonitrile within 60 min at a flow rate of 1.0 mL·min−1. The ultraviolet spectra were recorded at 350 nm for the identification of the two flavonoids. The contents of luteolin and luteoloside were highest in T. officinale flowers (5.817 ± 0.030 mg·g−1) and in T. coreanum leaves (6.205 ± 0.087 mg·g−1), respectively. The total content of luteolin and luteoloside in T. coreanum was about 1.3–2 times higher than that found in T. ohwianum and T. officinale.

Additional key words

isolation phytochemical quantitative analysis structure determination 

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Copyright information

© Korean Society for Horticultural Science 2011

Authors and Affiliations

  • Sullim Lee
    • 1
  • Saem Han
    • 1
  • Hye Min Kim
    • 1
  • Jeong Min Lee
    • 1
  • Jongkee Kim
    • 1
  • Chun-Geon Park
    • 2
  • Sanghyun Lee
    • 1
  1. 1.Department of Integrative Plant ScienceChung-Ang UniversityAnseongKorea
  2. 2.Department of Herbal Crop ResearchNational Institute of Horticultural & Herbal ScienceEumseongKorea

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