Horticulture, Environment, and Biotechnology

, Volume 52, Issue 2, pp 113–120 | Cite as

The activities of catalase and ascorbate peroxidase in olive (Olea europaea L. cv. Gemlik) under low temperature stress

  • Asuman Cansev
  • Hatice Gulen
  • Atilla Eris
Research Report


In this study, one-year-old shoots of the olive (Olea europaea L.) cv. Gemlik were tested at artificial low temperatures (4, −5°C, −10°C, and −20°C) every month for two years. For low temperature treatment, the degree of cell membrane injury in leaves and barks was determined by ion leakage method. In addition, with regard to antioxidative defense mechanism, activities of catalase (CAT, EC and ascorbate peroxidase (APX, EC enzymes were determined. Leaf and bark tissues subjected to 4°C and −5°C injured to a limited extent in all months. However, more than 50% injury occurred by temperatures equal to or colder than −10°C treatments depending on the season. For −10°C and −20°C treatments, the lowest and the highest injury in leaf and bark tissues were detected during winter and summer seasons, respectively. We determined in this study that CAT and APX enzyme activities are generally higher during fall and winter compared with those in summer. On the other hand, CAT and APX enzyme activities started increasing during fall along with a decreasing freezing injury while the activities of these enzymes decreased to some extent during winter when freezing injury was the lowest. In addition, while CAT activity decreased with low temperature treatments, APX activity did not change until −5°C treatment but decreased with decreasing temperatures starting from −10°C depending on the month the tissue was obtained. In conclusion, olive plant shows considerable tolerance to low temperatures that are achieved after daily gradual decreases by increasing cell membrane stability through complicated mechanisms including antioxidative enzyme metabolisms. In addition, APX may be more effective in maintaining cold-hardiness of olive compared with CAT.

Additional key words

antioxidative enzymes antioxidative mechanisms cold acclimation evergreen woody plants freezing injury 


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Literature Cited

  1. Anderson, M.D., T.K. Prasad, and C.R. Stewart. 1995. Changes in isoenzyme profiles of catalase, peroxidase and glutathione reductase during acclimation to chilling in mesocotyls of maize seedlings. Plant Physiol. 109:1247–1257.PubMedGoogle Scholar
  2. Arora, R., M.E. Wisniewski, and R. Scorza. 1992. Cold acclimation in genetically related (Sibling) dedicious and evergreen peach (Prunus persica [L.] Batsch). I. seasonal changes in cold hardiness and polypeptides of bark and xylem tissues. Plant Physiol. 99: 1562–1568.PubMedCrossRefGoogle Scholar
  3. Asada, K. 1992. Ascorbate peroxidase a hydrogen peroxide scavenging enzyme in plants. Physiologia Plantarum 85:235–241.CrossRefGoogle Scholar
  4. Baek, K.H. and D.Z. Skinner. 2003. Alteration of antioxidant enzyme gene expression during cold acclimation of near-isogenic wheat lines. Plant Science 165:1221–1227.CrossRefGoogle Scholar
  5. Bartolozzi, F., P. Rocchi, F. Camerini, and G. Fontanazza. 1999. Changes of biochemical parameters in olive (Olea europaea L.) leaves during an entire vegetative season, and their correlation with frost resistance. Acta Hort. 474:435–440.Google Scholar
  6. Bowler, C., M. Montagu, and D. Inze. 1992. Superoxide dismutase and stress tolerance. Annu. Rev. Plant Physiol. Plant Mol. Biol. 43:83–116.CrossRefGoogle Scholar
  7. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248–254.PubMedCrossRefGoogle Scholar
  8. Cansev, A., H. Gulen, A. Ipek, and A. Eris. 2006. Seasonal changes in various enzyme activities in olive genotypes. Plant Biology Meeting, August 5–9 2006, Boston, MA, USA p. 140.Google Scholar
  9. Cansev, A., H. Gulen, and A. Eris. 2009. Cold-hardiness of olive (Olea europaea l.) cultivars in cold-acclimated and non-acclimated stages: seasonal alteration of antioxidative enzymes and dehydrin-like proteins. J Agr. Sci. 147:51–61.CrossRefGoogle Scholar
  10. Chamnongpol, S., H. Willekens, W. Moeder, C. Langebartels, H. Sandermann, M. Van Montagu, D. Inze, and W. Van Camp. 1998. Defense activation and enhanced pathogen tolerance induced by H2O2 in transgenic tobacco. PNAS USA 95:5818–5823.PubMedCrossRefGoogle Scholar
  11. Chen, Y., M. Zhang, T. Chen, Y. Zhang, and Y. An. 2006. The relationship between seasonal changes in anti-oxidative system and freezing tolerance in the leaves of evergreen woody plants of Sabina. S. Afr. J. Bot. 72:272–279.CrossRefGoogle Scholar
  12. Cho, U.H. and J.O. Park. 2000. Mercury-induced oxidative stress in tomato seedlings. Plant Sci. 156:1–9.PubMedCrossRefGoogle Scholar
  13. D’angeli, S. and M.M. Altamura. 2007. Osmatin induces cold protection in olive trees by affecting programmed cell death and cytosketelon organization. Planta 225:1147–1163.PubMedCrossRefGoogle Scholar
  14. Dat, J., S. Vandenabeele, E. Vranova, M. Van Montagu, D. Inze, and F. Van Breusegem. 2000. Dual action of the active oxygen species during plant stress responses. CMLS 57:779–795.PubMedCrossRefGoogle Scholar
  15. Davis, G.D. and H.R. Swanson. 2001. Activity of stress-related enzymes in the perennial weed leafy spurge (Euphorbia esula L.). Environ. Exper. Bot. 46:95–108.CrossRefGoogle Scholar
  16. Eris, A., H. Gulen, E. Barut, and A. Cansev. 2007. Annual patterns of total soluble sugars and proteins related to cold hardiness in olive (Olea europaea L. cv. Gemlik). J. Hortic. Sci. Biotech. 82:597–604.Google Scholar
  17. Foyer, C.H. 1993. Ascorbic acid. p. 31–58. In: R.C. Alscher, and J.L. Hess (eds.) Antioxidants in higher plants. CRC Press, FL.Google Scholar
  18. Fridovich, I. 1978. The biology of oxygen radicals. Science 201:875–879.PubMedCrossRefGoogle Scholar
  19. Gulen, H. and A. Eris. 2004. Effect of heat stress on peroxidase activity and total protein content in strawberry plants. Plant Sci. 166:739–744.CrossRefGoogle Scholar
  20. Gómez-Del-Compo, M. and D. Barranco. 2005. Field evaluation of frost tolerance in 10 olive cultivars. Plant Gen. Resour. 3:385–390.CrossRefGoogle Scholar
  21. Guo, F.-X., M.X. Zhang, Y. Chen, W.-H. Zhang, S.-J. Xu, J.H. Wang, and L.Z. An. 2006. Relation of several antioxidant enzymes to rapid freezing resistance in suspension cultures cells from Alpine Chorispora bungeana. Cryobiology 52:241–250.PubMedCrossRefGoogle Scholar
  22. Gusta, L.V., M. Wisniewski, N.T. Nesbitt, and K.T. Tanino. 2003. Factors to consider in artificial freeze tests. Acta Hort. 618:493–507.Google Scholar
  23. Halliwell, B. and J.M.C. Gutteridge. 1986. Oxygen free radicals and iron in relation to biology and medicine: some problems and concepts. Arch. Biochem. Biophys. 246:501–514.PubMedCrossRefGoogle Scholar
  24. Havir, E.A. and N.A. Mchale. 1987. Biochemical and developmental characterization of multiple forms of catalase in tobacco leaves. Plant Physiol. 84:450–455.PubMedCrossRefGoogle Scholar
  25. Iturbe-Ormaetxe, I., P.R. Escuredo, C. Arrese-Igor, and M. Becana. 1998. Oxidative damage in pea plants exposed to water deficit or paraquat. Plant Physiol. 116:173–181.CrossRefGoogle Scholar
  26. Kendall E.J. and B.D. Mckersie. 1989. Free radical and freezing injury to cell membranes of winter wheat. Physiol. Plant 76:86–94.CrossRefGoogle Scholar
  27. Kuk, Y.I., J.S. Shin, N.R. Burgos, T.E. Hwang, O.H. Han, B.H. Cho, S. Jung, and J.O. Guh. 2003. Antioxidative enzymes offer protection from chilling damage in rice plants. Crop Sci. 43:2109–2117.CrossRefGoogle Scholar
  28. Lindén, L. 2002. Measuring cold hardening in woody plants. University of Helsinki, Department of Applied Biology, Publication no. 10. Helsinki.Google Scholar
  29. Mahajan, S. and N. Tuteja. 2005. Cold, salinity and drought stresses. An overview. Arch. Biochem. Biophys. 444:139–158.PubMedCrossRefGoogle Scholar
  30. Moran, J.F., M. Becana, I. Iturbe-Ormaetxe, S. Frechilla, R.V. Klucas, and P. Aparicio-Tejo. 1994. Drought induces oxidative stress in pea plants. Planta 194:346–352.CrossRefGoogle Scholar
  31. Nakano, Y. and K. Asada. 1980. Spinach chloroplasts scavenge hydrogen peroxide on illumination. Plant Cell Physiol. 21:1295–1307.Google Scholar
  32. O’kane, D., V. Gill, P. Boyd, and R. Burdon. 1996. Chilling oxidative stress and antioxidant responses in Arabidopsis thaliana callus. Planta 198:371–377.PubMedCrossRefGoogle Scholar
  33. Pacifici, R.E. and K.J.A. Davies. 1990. Protein degradation as an index of oxidative stress. Methods Enzymol. 186:485–502.PubMedCrossRefGoogle Scholar
  34. Palliotti, A. and G. Bongi. 1996. Freezing injury in the olive leaf and effects of mefluidide treatment. J. Horticul. Sci. 71:57–63.Google Scholar
  35. Parvanova, D, S. Ivanov, T. Konstantinova, E. Karanov, A. Atanassov, T. Tsvetkov, V. Alexiieva, and D. Djilanov. 2004. Transgenic tabocco plants accumulating osmolytes show reduced oxidative damage under freezing stress. Plant Physiol. Biochem. 42:57–63.PubMedCrossRefGoogle Scholar
  36. Prasad, T.K. 1996. Mechanisms of chilling-induced oxidative stress injury and tolerance: changes in antioxidant system, oxidation of proteins and lipids and protease activities. Plant J. 10:1017–1026.CrossRefGoogle Scholar
  37. Prasad, T.K. 1997. Role of catalase in inducing chilling tolerance in pre-emergence maize seedlings. Plant Physiol. 114:1369–1376.PubMedGoogle Scholar
  38. Prasad, T.K., M.D. Anderson, B.A. Martin, and C.R. Stewart. 1994. Evidence for chilling-induced oxidative stress in maize seedlings and a regulatory role for hydrogen peroxide. Plant Cell 6:65–74.PubMedCrossRefGoogle Scholar
  39. Rao, M.V., G. Paliyath, and D.P. Ormrod. 1996. Ultraviolet-B- and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana. Plant Physiol. 110:125–136.PubMedCrossRefGoogle Scholar
  40. Saruyama, H. and M. Tanida. 1995. Effect of chilling on activated oxygen-scavenging enzymes in low temperature-sensitive and -tolerant cultivars of rice (Oryza sativa L.). Plant Sci. 109:105–113.CrossRefGoogle Scholar
  41. Scandalios, J.G. 1993. Oxygen stress and superoxide dismutases. Plant Physiology 101:7–12.PubMedGoogle Scholar
  42. Sudhakar, C., A. Lakshmi, and S. Giridarakumar. 2001. Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Morus alba L.) under NaCl salinity. Plant Sci. 161:613–619.CrossRefGoogle Scholar
  43. Steponkus, P.L. 1984. Role of plasma membrane in freezing injury and cold-acclimation. Annu. Rev. Plant Phys. Plant Mol. Biol. 35:543–584.CrossRefGoogle Scholar
  44. Tao, D.L., G. Öquist, and G. Wingsle. 1998. Active oxygen scavengers during cold acclimation of Scots pine seedlings in relation to freezing tolerance. Cryobiology 37:38–45.PubMedCrossRefGoogle Scholar
  45. Walker, M.A. and B.D. Mckersie. 1993. Role of ascorbate-glutathione antioxidant system in chilling resistance of tomato. J Plant Physiol. 141:234–239.Google Scholar
  46. Yoshida, S. and M. Uemura. 1990. Responses of the plasma membrane to cold acclimation and freezing stress. In: Larsson, C.H. and I.M. Møller (eds.): The plant plasma membrane. Springer, Berlin, 293–320.Google Scholar
  47. Zhou, R. and H. Zhao. 2004. Seasonal pattern of antioxidant enzyme system in the roots of perennial forage grasses grown in alpine habitat, related to freezing tolerance. Physiol. Plant 121:399–408.CrossRefGoogle Scholar

Copyright information

© Korean Society for Horticultural Science 2011

Authors and Affiliations

  1. 1.Horticulture DepartmentFaculty of Agriculture Uludag UniversityBursaTurkey

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