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Effects of light intensity and relative humidity on photosynthesis, growth and graft-take of grafted cucumber seedlings during healing and acclimatization

  • Yoonah Jang
  • Eiji Goto
  • Yasuhiro Ishigami
  • Boheum Mun
  • Changhoo Chun
Research Report

Abstract

Healing and acclimatization are key processes for the survival of grafted plants. This study evaluated the influence of light intensity (photosynthetic photon flux, PPF) and relative humidity during the healing and acclimatization period on the photosynthetic characteristics, graft-take, and growth of grafted cucumber (Cucumis sativus L.) seedlings, using a system for the continuous measurement of the CO2 exchange rate, in order to establish optimum environmental conditions for the healing and acclimatization of grafted cucumbers seedlings. Cucumbers (Cucumis sativus L. cv. Joeun Baekdadaki) were grafted onto rootstocks (Cucurbita maxima D. × C. moshata D. cv. New Shintozwa). Six combinations of two levels of relative humidity (95 and 90%) and three levels (0, 142, and 237 μmol·m−2·s−1) of light intensity were set up during healing and acclimatization. Increasing light intensity significantly increased CO2 exchange rates during healing and acclimatization. At 95 and 90% relative humidity, the CO2 exchange rates at 237 μmol·m−2·s−1 light intensity were 1.5 and 1.8 times higher than those at 142 μmol·m−2·s−1 light intensity, respectively. The light intensity during healing and acclimatization also affected the amount and distribution of chloroplasts in scion cotyledon. The amount of chloroplasts increased with the increase of PPF during healing and acclimatization, which covered most of cell wall with little open space left, compared with that of dark condition. As PPF increased, the shoot length, ratio of shoot to root, and specific leaf area decreased but the hypocotyl diameter, leaf area, dry weight, and percent dry matter increased. On the other hand, the relative humidity ranging from 90 to 95% did not significantly affect the CO2 exchange rates during healing, acclimatization, and growth of grafted cucumber seedlings. As a result, PPF during healing and acclimatization affected the growth and quality of grafted cucumber seedlings. This showed that higher PPF condition may improve the growth and quality of grafted cucumber seedlings.

Additional key words

CO2 exchange rate Cucumis sativus L. Cucurbita maxima D. × C. moshata D. graft 

Literature Cited

  1. Kim, Y.H. 2000. Effects of air temperature, relative humidity, and photosynthetic photon flux on the evapotranspiration rate of grafted seedlings under artificial lighting. p. 91–97. In: C. Kubota and C. Chun (eds.). Transplant production in the 21st century. Kluwer Academic Publishers, The Netherlands.Google Scholar
  2. Kim, Y.H. and H.S. Park. 2001. Evapotranspiration rate of grafted seedlings affected by relative humidity and photosynthetic photon flux under artificial lighting. J. Kor. Soc. Agricultural Machinery 26:379–384.Google Scholar
  3. ai]Kim, Y.H., C.S. Kim, J.W. Lee, and S.G. Lee. 2001. Effect of vapor pressure deficit on the evaportranspiration rate and graft-taking of grafted seedlings population under artificial lighting. J. Bio-Environ. Control 10:232–236.Google Scholar
  4. Kitaya, Y., N. Genhua, K. Toyoki, and M. Ohashi. 1998. Photosynthetic photon flux, photoperiod, and CO2 concentration affect growth and morphology of lettuce plug transplants. Hortscience 33:988–991.Google Scholar
  5. Kitaya, Y. 2005. Photosynthesis and environments. p. 104–105. In: T. Nagano and K. Omasa (eds.). Agricultural meteorology environmentology. Asakurashoten, Tokyo.Google Scholar
  6. Kozai, T. and C. Chun. 2002. Closed systems with artificial lighting for production of high quality transplants using minimum resource and environmental pollution. Acta Hort. 578:27–33.Google Scholar
  7. Kozai, T., C. Chun, and K. Ohyama, 2004. Closed system with lamps for commercial production of transplants using minimal resources. Acta Hort. 630:239–254.Google Scholar
  8. Lee, J. M., and M. Oda. 2003. Grafting of herbaceous vegetable and ornamental crops. Hort. Rev. 28:61–121.Google Scholar
  9. Lee, N., H.Y. Wetzstein, and H.E. Sommer. 1985. Effects of quantum flux density on photosynthesis and chloroplast ultrastructure in tissue-cultured plantlets and seedlings of Liquidambar styraciflua L. towards improved acclimatization and field survival. Plant Physiol. 75:637–641.CrossRefGoogle Scholar
  10. Li, Q., M. Deng, J. Chen, and R.J. Henny. 2009. Effects of light intensity and paclobutrazol on growth and interior performance of Pachira aquatic Aubl. Hortscience 44:1291–1295.Google Scholar
  11. Long, S.P., P.K. Farage, and R.L. Garcia. 1996. Measurement of leaf and canopy photosynthetic CO2 exchange in the field. J. Experimental Bot. 47:1629–1642.CrossRefGoogle Scholar
  12. Luft, J.H. 1973. Embedding media-old and new. p. 1–34, In: J.K. Koehler (ed.). Advance techniques in biological electron microscopy. Springer-Verlag, Berlin and New York.Google Scholar
  13. McMillen, G.G. and J.H. McClendon. 1983. Dependence of photosynthetic rates on leaf density thickness in deciduous woody plants grown in sun and shade. Plant Physiol. 72:674–678.PubMedCrossRefGoogle Scholar
  14. Nobuoka, T., M. Oda, and H. Sasaki. 1996. Effects of relative humidity, light intensity, and leaf temperature on transpiration of tomato scions. J. Japan Soc. Hort. Sci. 64:859–865.CrossRefGoogle Scholar
  15. Nobuoka, T., T. Nishimoto, and K. Toi. 2005. Wind and light promote graft-take and growth of grafted tomato seedlings. J. Japan Soc. Hort. Sci. 74:170–175.CrossRefGoogle Scholar
  16. Oguchi, R., K. Hikosaka, and T. Hirose. 2003. Does the photosynthetic light-acclimation need change in leaf anatomy? Plant Cell Environ. 26:505–512.CrossRefGoogle Scholar
  17. Rivero, R.M., J.M. Ruiz, and L. Romero. 2003. Role of grafting in horticultural plants under stress conditions. Food Agr. Environ. 1:70–74.Google Scholar
  18. Shibuya, T. and T. Kozai. 1998. Effects of air current speed on net photosynthetic and evapotranspiration rates of a tomato plug sheet under artificial light. Environ. Control Biol. 36:131–136.Google Scholar
  19. Shibuya, T., S. Kawaguchi, T. Seike, and M. Kiyota. 2003. Effects of opening and closing of a plastic tunnel on microclimate and gas exchange of a grafted tomato-transplant community during the acclimatization stage. Environ. Control Biol. 41:301–306.Google Scholar
  20. Shibuya, T., J. Tsuruyama, Y. Kitaya, and M. Kiyota. 2006. Enhancement of photosynthesis and growth of tomato seedlings by forced ventilation within the canopy. Scientia Hort. 109:218–222.CrossRefGoogle Scholar
  21. van Iersel, M.W. and B. Bugbee. 2000. A multi-chamber, semi-continuous, crop carbon dioxide exchange system: Design, calibration, and data interpretation. J. Amer. Soc. Hort. Sci. 125:86–92.Google Scholar

Copyright information

© Korean Society for Horticultural Science 2011

Authors and Affiliations

  • Yoonah Jang
    • 1
  • Eiji Goto
    • 2
  • Yasuhiro Ishigami
    • 2
  • Boheum Mun
    • 3
  • Changhoo Chun
    • 4
  1. 1.National Institute of Horticultural & Herbal ScienceRural Development AdministrationSuwonKorea
  2. 2.Department of Bioprodution Science, Faculty of HorticultureChiba UniversityMatsudo, ChibaJapan
  3. 3.Research Coordination DivisionRural Development AdministrationSuwonKorea
  4. 4.Department of Plant Science, College of Agriculture and Life SciencesSeoul National UniversitySeoulKorea

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