Human Cell

, Volume 33, Issue 1, pp 10–22 | Cite as

Increased in vitro migration of human umbilical cord mesenchymal stem cells toward acellular foreskin treated with bacterial derivatives of monophosphoryl lipid A or supernatant of Lactobacillus acidophilus

  • Ali Shojaeian
  • Ameneh Mehri-Ghahfarrokhi
  • Mehdi Banitalebi-DehkordiEmail author
Research Article


Migration and homing are known as critical steps toward regeneration of damaged tissues via cell therapies. Among various cellular sources of stem cells, the umbilical cord has been thus recognized as an interesting one endowed with high benefits. Accordingly, the main objective of the present study was to determine whether monophosphoryl lipid A (MPLA) or supernatant of Lactobacillus acidophilus (SLA) could increase migration of human umbilical cord mesenchymal stem cells (hUMSCs) toward acellular foreskin or not. In this study, the hUMSCs were isolated and cultured through acellular MPLA- or SLA-treated foreskin. Expression of some migration genes (i.e., VCAM-1, MMP-2, VLA-4, CXCR-4, and VEGF) was also investigated using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Moreover; vimentin, cytokeratin 5 (CK5), and matrix metalloproteinases-2 (MMP-2) were detected via immunohistochemistry (IHC) analysis. The hUMSCs in the presence of MPLA- or SLA-treated foreskin showed more tissue tropism compared with those in the control group. Besides, the scanning electron microscopy (SEM) results established that the hUMSCs had more migratory activity in the presence of MPLA- or SLA-treated foreskin than the untreated one. The IHC analysis results correspondingly indicated that expression of vimentin, CK5, and MMP-2 proteins had augmented in both treatments compared with those in the control group. It was concluded that MPLA had revealed more prominent results than SLA, even though both treatments could be regarded as inducing factors in migration. Ultimately, it was suggested to introduce the use of MPLA and probiotic components as a promising approach to improve therapies in regenerative medicine.


Human umbilical cord mesenchymal stem cells Monophosphoryl lipid A Lactobacillus acidophilus Acellular foreskin Migration 



The authors would like to express sincere thanks to the staffs of Cellular and Molecular Research Center of Basic Health Sciences Institute of Shahrekord University of Medical Sciences for their cooperation. This research project was extracted from a Ph.D. thesis and was supported by a deputy of research and technology of Shahrekord University of Medical Sciences (SKUMS) [Grant Number: 2757].

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All experiments were approved by the Ethics and Clinical Studies Research Committee of SKUMS according to Helsinki Declaration (IR.SKUMS.REC.1397.80).

Informed consent

Informed consents were obtained from all mothers before surgery.


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Copyright information

© Japan Human Cell Society and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  • Ali Shojaeian
    • 1
  • Ameneh Mehri-Ghahfarrokhi
    • 1
  • Mehdi Banitalebi-Dehkordi
    • 2
    Email author
  1. 1.Department of Molecular Medicine, School of Advanced TechnologiesShahrekord University of Medical SciencesShahrekordIran
  2. 2.Cellular and Molecular Research Center, Basic Health Sciences InstituteShahrekord University of Medical SciencesShahrekordIran

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