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miR-29b inhibits non-small cell lung cancer progression by targeting STRN4

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Abstract

Non-small cell lung cancer (NSCLC) is a malignant tumor with a high fatality, low overall cure, and survival rates worldwide. When only palliative therapy is available, the disease leads to malignant proliferation. Previous studies showed miR-29b serves as an NSCLC suppressor by inhibiting cells proliferation, migration, and invasion. However, the mechanism underlying NSCLC progression remains elusive. In this study, we identified Striatin 4 (STRN4), a target of miR-29b, which serves as a pro-oncogenic protein by promoting cells proliferation, migration, and invasion in NSCLC. Besides, the STRN4 was highly expressed in NSCLC and negatively regulated by miR-29b. Down-regulation of STRN4 inhibits NSCLC cells proliferation, migration, invasion, and promotes apoptosis in vitro, whereas overexpression-induced enhanced cell migration and invasion could be reverved by miR-29b. Notably, overexpression of miR-29b and down-regulation of STRN4 by shRNA suppressed cellular proliferation and delayed tumor progression in vivo. Together, these findings identify a miR-29b/STRN4 regulatory pathway in NSCLC progression, which may provide a new sight for the treatment of NSCLC.

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Data accessibility

All data necessary to understand and assess the conclusions of this study are available in the main text. There are no restrictions on data availability in the manuscript.

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Acknowledgements

We are grateful to the supporting from the Science and Technology Program for Public Wellbeing of Chengdu (2015-HM01-00224-SF). We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

Author information

YX participated in the research design. YX and FZ conducted the experiments. PZ and DP performed data analysis. YX and YS wrote the manuscript.

Correspondence to Yangmei Shen.

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The authors declare no potential conflicts of interest.

Ethical approval

Written informed consent was obtained from all the participants. The protocols for human specimen studies were approved by the Ethics Committee of the West China Second University Hospital of Sichuan University, and were conducted in agreement with the principles set forth in the Declaration of Helsinki. All animal protocols were approved by the Animal Care and Use Committee of the West China Second University Hospital of Sichuan University.

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Cite this article

Xie, Y., Zhao, F., Zhang, P. et al. miR-29b inhibits non-small cell lung cancer progression by targeting STRN4. Human Cell 33, 220–231 (2020). https://doi.org/10.1007/s13577-019-00305-w

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Keywords

  • miR-29b
  • STRN4
  • Non-small cell lung cancer
  • Progression
  • Overexpression