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Establishment and characterization of novel patient-derived extraskeletal osteosarcoma cell line NCC-ESOS1-C1

Abstract

Extraskeletal osteosarcoma (ESOS) is a rare mesenchymal malignancy producing osteoid and bone in soft tissue without skeletal attachment. ESOS exhibits chemoresistance and poor prognosis, and is distinct from osseous osteosarcoma. The biological characteristics of ESOS are not fully understood, and patient-derived cell lines of ESOS are not available from public cell banks. Here, we established a novel cell line of ESOS and characterized its genetic and biological characteristics as well as examined its response to anti-cancer reagents. The cell line was established using tumor tissue from a 58-year-old female patient with ESOS, and named as NCC-ESOS1-C1. Phenotypes relevant to malignancy such as proliferation and invasion were examined in vitro, and genetic features were evaluated using the NCC Oncopanel assay. The response to inhibitors was monitored by screening of an anti-cancer reagent library. The cells constantly proliferated, showing spheroid formation and invasion capabilities. The NCC Oncopanel revealed the presence of actionable mutations in PIK3CA. Library screening revealed the presence of anti-cancer reagents with significant anti-proliferative effects on NCC-ESOS1-C1 at a low concentration. In conclusion, we established and characterized a novel ESOS cell line, NCC-ESOS1-C1. This cell line will be a useful resource for basic research and preclinical studies.

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Acknowledgements

We thank Drs. Y. Minami, K. Shimizu, T. Mori, T. Uehara M. Sugawara, Y. Araki, S. Toki, T. Hirose, J. Sugaya, and Ms. R. Nakano, Division of Musculoskeletal Oncology, National Cancer Center Hospital, for sampling tumor tissue specimens from surgically resected materials. We also appreciate the technical assistance by Mrs. A. Sei, Division of Rare Cancer Research. We would like to thank Editage (www.editage.jp) for English-language editing and their constructive comments on the manuscript. This research was financially supported by the National Cancer Center Research and Development Fund (Grant no. 29-A-2), and technically assisted by the Fundamental Innovative Oncology Core in the National Cancer Center.

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Correspondence to Tadashi Kondo.

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Supplementary material 8: Supplementary Fig. 1 Representative images of the invasion assay in the cell lines NCC-ESOS1-C1 and MG63. AD Representative images of the invasion assay in NCC-ESOS1-C1 A: 1 × 105 cells after 24 h; B: 2 × 105 cells after 24 h; C: 1 × 105 cells after 48 h; and D: 2 × 105 cells after 48 h. EH: Representative images of the invasion assay in MG63. E: 1 × 105 cells after 24 h; F: 2 × 105 cells after 24 h; G: 1 × 105 cells after 48 h; and H: 2 × 105 cells after 48 h. Scale bar indicates 200 µm. (TIFF 1606 kb)

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Kito, F., Oyama, R., Noguchi, R. et al. Establishment and characterization of novel patient-derived extraskeletal osteosarcoma cell line NCC-ESOS1-C1. Human Cell 33, 283–290 (2020). https://doi.org/10.1007/s13577-019-00291-z

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Keywords

  • Extraskeletal osteosarcoma
  • Patient-derived cancer cell line
  • Drug screening
  • Actionable genes
  • NCC Oncopanel