Long noncoding RNAs (lncRNAs) have been found to be abnormally expressed in cancer, and lncRNA small nucleolar RNA host genes (SNHGs) play critical roles in tumour progression. SNHG3 has been identified as an oncogene in multiple tumour types. However, the role of SNHG3 in breast cancer has not been reported. In this study, we found that SNHG3 was upregulated and associated with tumour malignancy in patients with breast cancer. SNHG3 knockdown inhibited the growth and metastatic capabilities of breast cancer cells in vitro and vivo. We used bioinformatics prediction and functional assay validation to determine that SNHG3 upregulation inhibited miR-384 activity and led to hepatoma-derived growth factor (HDGF) overexpression in breast cancer cells. The findings of this study show that SNHG3 functions as an oncogene in breast cancer and promotes breast cancer cell proliferation and invasion by regulating the miR-384/HDGF axis. The present study might provide a new target for the treatment of breast cancer.
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This work was supported by the Medicine and Health Science Technology Development Projects of Shandong Province (No. 2016WSO749).
Conflict of interest
The authors declare that they have no conflict of interest.
Ethical consent was granted from the Medical Ethics Committee of Zibo Central Hospital for studies involving patients. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. Ethical approval of the animal study was granted by the Animal Ethics Committee of Zibo Central Hospital.
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Fig. S1 si-SNHG3-1 inhibits breast cancer cell proliferation, migration and invasionin vitro.(a) qRT-PCR verified the interference efficiency of SNHG3-1 in breast cancer cell lines. (b) A CCK-8 assay was performed to measure the proliferation of breast cancer cells after transfection with si-SNHG3-1 or si-NC. (c) A colony formation assay was performed to measure the proliferation of breast cancer cells after transfection. (d) Transwell assays were performed to determine the effects of SNHG3 knockdown on breast cancer cell migration and invasion. The experiments were performed independently in triplicate, and the data are presented as the mean ± SEM, ***P <0.001. (JPEG 858 kb)
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Ma, Q., Qi, X., Lin, X. et al. LncRNA SNHG3 promotes cell proliferation and invasion through the miR-384/hepatoma-derived growth factor axis in breast cancer. Human Cell 33, 232–242 (2020) doi:10.1007/s13577-019-00287-9
- LncRNA SNHG3
- Breast cancer