Human Cell

, Volume 33, Issue 1, pp 29–36 | Cite as

Integrins expressed on the surface of human endometrial stromal cells derived from a female patient experiencing spontaneous abortion

  • Jie Ohn Sohn
  • Hye Jin Park
  • Se Hee Kim
  • Min Ji Kim
  • Hyun Jin Song
  • Jung Im Yun
  • Jeong Mook LimEmail author
  • Seung Tae LeeEmail author
Research Article


Here, as a basic study in revealing the correlation between extracellular matrix components and spontaneous abortion, we defined the types of integrins expressed on the surface of endometrial stromal (ES) cells retrieved from the uterus of a patient experiencing spontaneous abortion. For these, the types of integrin subunits in the ES cells retrieved from a woman with spontaneous abortion were identified at the transcriptional and translational levels, and functional assay was conducted for confirming the combinations of integrin α and β subunits. Among the genes encoding 25 integrin subunits, significantly high transcription was seen in integrins α1, α2, α3, α4, α5, αV, β1, β3, and β5. Translation of integrins α1, α3, α5, αV, and β1 on the cell surface was detected in almost all ES cells, whereas integrins α2, α4, β3, and β4 were expressed translationally only in some ES cells. Subsequently, ES cells showed significantly increased adhesion to collagen I, laminin, fibronectin, and vitronectin, and functional blocking of integrin α1, α3, α5, and αV significantly inhibited adhesion to these molecules. These results demonstrated that active heterodimers composed of integrins α1β1, α3β1, α5β1, and αVβ1 were co-localized on the surface of ES cells derived from a patient experiencing spontaneous abortion.


Human Infertility Spontaneous abortion Endometrial stromal cell Integrins 



This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICR and Future Planning (NRF-2017R1A2B4009777).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional review board of Seoul Women’s Hospital as well as the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

13577_2019_278_MOESM1_ESM.docx (22 kb)
Supplementary material 1 (DOCX 21 kb)
13577_2019_278_MOESM2_ESM.docx (16 kb)
Supplementary material 2 (DOCX 16 kb)


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Copyright information

© Japan Human Cell Society and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Agricultural BiotechnologySeoul National UniversitySeoulSouth Korea
  2. 2.Department of Animal Life ScienceKangwon National UniversityChuncheonSouth Korea
  3. 3.Institute of Animal ResourcesKangwon National UniversityChuncheonSouth Korea
  4. 4.Fertility Medical CenterSeoul Women’s HospitalBucheonSouth Korea
  5. 5.Laboratory of Stem Cell Biomodulation, Department of Applied Animal ScienceKangwon National UniversityChuncheonSouth Korea
  6. 6.Research Institute of Agriculture and Life SciencesSeoul National UniversitySeoulSouth Korea

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