Human Cell

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Long noncoding RNA LINC00460 aggravates invasion and metastasis by targeting miR-30a-3p/Rap1A in nasopharyngeal carcinoma

  • Xiaodong HuEmail author
  • Weiwei Liu
  • Xue Jiang
  • Bin Wang
  • Liping Li
  • Jian Wang
  • Jinhua Ma
Research Article


Accumulating studies have shown that long noncoding RNAs (lncRNAs) are involved in cancer occurrence and development. Recently a new lncRNA LINC00460 has been reported to be upregulated in several types of cancers. In this study, we investigated the role of LINC00460 in invasion and metastasis of nasopharyngeal carcinoma (NPC). In NPC cell lines named C666-1 and 5-8F, LINC00460 overexpression promoted cancer cell migration, invasion and epithelial-mesenchymal transition (EMT), while depletion of LINC00460 exhibited opposite effects. We further demonstrated that LINC00460 regulated Rap1A expression through competitively binding to miR-30a-3p. LINC00460 knockdown suppressed NPC metastasis in a xenograft mouse model. Taken together, our findings suggested LINC00460 functioned as an oncogene in NPC which promoted invasion and metastasis, shedding lights on LINC00460 as a potential therapeutic target for NPC therapeutics from bench to clinic.


LINC00460 miR-30a-3p Rap1A Nasopharyngeal carcinoma 


Author contributions

XDH and WWL conceived and designed the experiments, XL and BW analyzed and interpreted the results of the experiments, LPL, JW and JHM performed the experiments.

Compliance with ethical standards

Availability of data and materials

All data generated or analyzed during this study are included in this published article.

Informed consent

Written informed consent was obtained from a legally authorized representative(s) for anonymized patient information to be published in this article.

Conflict of interest

Authors declared that there were no conflicts of interests.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. Ethical approval regarding animal study was granted from the Animal Ethics Committee of Cangzhou Central Hospital. Ethical consent was granted from the Medical Ethics Committee of Cangzhou Central Hospital for study involving patients.

Supplementary material

13577_2019_262_MOESM1_ESM.tif (41 kb)
Supplementary material 1 (TIFF 41 kb)
13577_2019_262_MOESM2_ESM.tif (40 kb)
Supplementary material 2 (TIFF 40 kb)


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Copyright information

© Japan Human Cell Society and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  • Xiaodong Hu
    • 1
    Email author
  • Weiwei Liu
    • 1
  • Xue Jiang
    • 1
  • Bin Wang
    • 1
  • Liping Li
    • 1
  • Jian Wang
    • 1
  • Jinhua Ma
    • 1
  1. 1.E.N.T. DepartmentCangzhou Central HospitalCangzhouPeople’s Republic of China

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