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Human Cell

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Classical differentiation protocols upregulate the expression of the axon guidance genes PLXNA2 and SEMA3C in SH-SY5Y neuroblastoma cells

  • Ivan PrestaEmail author
  • Annalidia Donato
  • Natalia Malara
  • Emilio Russo
  • Marta Letizia Hribal
  • Giuseppe Donato
Letter to the Editor
  • 26 Downloads

Dear Editor,

The neuroblastoma-derived SH-SY5Y cell line is extensively used in Parkinson’s disease molecular studies for evaluating neurotoxicity or testing neuroprotective effects of drugs [1]. Usually, differentiation is induced, before treatments, by exposure to diverse compounds, most commonly all-trans retinoic acid (atRA) or phorbol 12-myristate, 13-acetate (PMA), for 7 days at least [2]. Both atRA and PMA treatments entail the emission of cytoplasmic axon-like processes and changes in enzymatic activity and gene expression, toward that typical of adult neurons [3, 4]. In developing nervous system, class A plexins associate with neuropilins acting as eterodimeric receptors for the secreted class 3 semaphorins [5]. This plexins/semaphorins system is involved in axon navigation into the depths of tissues with diverse members of the class 3 semaphorins acting with repulsive or attractive effects. Here, we evaluated the expression of mRNA and protein levels of the PLXNA2 and SEMA3C...

Keywords

PlexinA2 Semaphorin3C Parkinson’s disease Neuronal differentiation SH-SY5Y 

Abbreviations

HRP

Horseradish peroxidase

BSA/TBS-T

Tris buffered saline plus 0.1% Tween 20

PBS

Phosphate-buffered saline

PBS-T

PBS + 0.1% Tween 20

RPS9

Ribosomal Protein S9

SDS-PAGE

Sodium dodecyl sulphate-polyacrylamide gel electrophoresis

FBS

Fetal bovine serum

Notes

Compliance with ethical standards

Conflict of interest

All authors declare they have no affiliations with or involvement in any organization with any financial or non-financial interest.

Supplementary material

13577_2019_246_MOESM1_ESM.docx (997 kb)
Supplementary material 1 (DOCX 997 KB)

References

  1. 1.
    Biedler JL, Schachner M. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 1978;38:3751–7.Google Scholar
  2. 2.
    Påhlman S, Ruusala AI, Abrahamsson L, Mattsson MEK, Esscher T. Retinoic acid-induced differentiation of cultured human neuroblastoma cells: a comparison with phorbolester-induced differentiation. Cell Differ. 1984;14:135–44.CrossRefGoogle Scholar
  3. 3.
    Xicoy H, Wieringa B, Martens GJM. The SH-SY5Y cell line in Parkinson’s disease research: a systematic review. Mol Neurodegener. 2017;12:1–11.CrossRefGoogle Scholar
  4. 4.
    Kunzler A, Zeidán-Chuliá F, Gasparotto J, Girardi CS, Klafke K, Petiz LL, et al. Changes in cell cycle and up-regulation of neuronal markers during SH-SY5Y neurodifferentiation by retinoic acid are mediated by reactive species production and oxidative stress. Mol Neurobiol. 2017;54:6903–16.CrossRefGoogle Scholar
  5. 5.
    Winberg ML, Noordermeer JN, Tamagnone L, Comoglio PM, Spriggs MK, Tessier-Lavigne M, et al. Plexin A is a neuronal semaphorin receptor that controls axon guidance. Cell. 1998;95:903–16.CrossRefGoogle Scholar
  6. 6.
    Pezzini F, Bettinetti L, Di Leva F, Bianchi M, Zoratti E, Carrozzo R, et al. Transcriptomic profiling discloses molecular and cellular events related to neuronal differentiation in SH-SY5Y neuroblastoma cells. Cell Mol Neurobiol. 2017;37:665–82.CrossRefGoogle Scholar
  7. 7.
    Hernández-Montiel HL, Tamariz E, Sandoval-Minero MT, Varela-Echavarría A. Semaphorins 3A, 3C, and 3F in mesencephalic dopaminergic axon pathfinding. J Comp Neurol. 2008;506:387–97.CrossRefGoogle Scholar
  8. 8.
    Hsu HH. Purification and partial characterization of ATP pyrophosphohydrolase from fetal bovine epiphyseal cartilage. J Biol Chem. 1983;258:3463–8.Google Scholar
  9. 9.
    Carballo-Molina OA, Sánchez-Navarro A, López-Ornelas A, Lara-Rodarte R, Salazar P, Campos-Romo A, et al. Semaphorin 3C released from a biocompatible hydrogel guides and promotes axonal growth of rodent and human dopaminergic neurons. Tissue Eng Part A. 2016;22:850–61.CrossRefGoogle Scholar

Copyright information

© Japan Human Cell Society and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Health SciencesUniversity “Magna Graecia” of Catanzaro, V.le EuropaCatanzaroItaly
  2. 2.Department of Medical and Surgical SciencesUniversity “Magna Graecia” of CatanzaroCatanzaroItaly
  3. 3.Department of Clinical and Experimental MedicineUniversity “Magna Graecia” of CatanzaroCatanzaroItaly

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