Advertisement

Human Cell

, Volume 32, Issue 1, pp 31–40 | Cite as

Down-regulation of lncTCF7 inhibits cell migration and invasion in colorectal cancer via inhibiting TCF7 expression

  • Baihe Wu
  • Meizhu Chen
  • Minzhao Gao
  • Yunyan Cong
  • Lifeng Jiang
  • Jinqi WeiEmail author
  • Jin HuangEmail author
Research Article

Abstract

The prognosis of colorectal cancer (CRC) is still very poor, owing to the high incidence of metastasis. Long noncoding RNA TCF7 (lncTCF7) has been shown to play critical roles in human CRC development and progression, but the molecular mechanisms of lncTCF7 in CRC are still unknown. This study aimed to explore the functions and molecular mechanisms of lncTCF7 on the migration and invasion of CRC cells. Notably, lncTCF7 was highly expressed in CRC cell lines relative to normal colonic epithelial cells. LncTCF7 knockdown significantly inhibited migration and invasion of CRC cells. In addition, TCF7 was highly expressed in CRC cell lines relative to that in normal colonic epithelial cells and its expression was significantly decreased in CRC cells transfected with si-lncTCF7. RNA immunoprecipitation, chromatin immunoprecipitation, and luciferase reporter assays showed that LncTCF7 recruits BAF170 to activate the TCF7 promoter and regulate TCF7 expression. TCF7 overexpression could promote migration and invasion in CRC cells transfected with si-lncTCF7, which reversed the effect of lncTCF7 on the migration and invasion of CRC cells. In conclusion, our data indicate that the downregulation of lncTCF7 significantly inhibits migration and invasion of CRC cells by inhibiting TCF7 expression, suggesting that lncTCF7 may be a potential target for CRC therapy.

Keywords

Long noncoding RNA LncTCF7 TCF7 BAF170 Colorectal cancer Migration Invasion 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants performed by any of the authors.

References

  1. 1.
    Siegel RL, Miller KD, Jemal A. Cancer statistics 2016. CA: Cancer J Clin. 2016;66(1):7–30.Google Scholar
  2. 2.
    Brenner H, Kloor M, Pox CP. Colorectal cancer. Lancet. 2014;383(9927):1490–502.CrossRefGoogle Scholar
  3. 3.
    Prenen H, Vecchione L, Van Cutsem E. Role of targeted agents in metastatic colorectal cancer. Target Oncol. 2013;8(2):83–96.CrossRefGoogle Scholar
  4. 4.
    Miller KD, Siegel RL, Lin CC, Mariotto AB, Kramer JL, Rowland JH, et al. Cancer treatment and survivorship statistics, 2016. CA: Cancer J Clin. 2016;66(4):271–89.Google Scholar
  5. 5.
    Siegel R, DeSantis C, Virgo K, Stein K, Mariotto A, Smith T, et al. Cancer treatment and survivorship statistics, 2012. CA: Cancer J Clin. 2012;62(4):220–41.Google Scholar
  6. 6.
    Nie FQ, Sun M, Yang JS, Xie M, Xu TP, Xia R, et al. Long noncoding RNA ANRIL promotes non-small cell lung cancer cell proliferation and inhibits apoptosis by silencing KLF2 and P21 expression. Mol Cancer Ther. 2015;14(1):268–77.CrossRefGoogle Scholar
  7. 7.
    Lu M, Liu Z, Li B, Wang G, Li D, Zhu Y. The high expression of long non-coding RNA PANDAR indicates a poor prognosis for colorectal cancer and promotes metastasis by EMT pathway. J Cancer Res Clin Oncol. 2017;143(1):71–81.CrossRefGoogle Scholar
  8. 8.
    Kogo R, Shimamura T, Mimori K, Kawahara K, Imoto S, Sudo T, et al. Long noncoding RNA HOTAIR regulates polycomb-dependent chromatin modification and is associated with poor prognosis in colorectal cancers. Can Res. 2011;71(20):6320–6.CrossRefGoogle Scholar
  9. 9.
    Khorkova O, Hsiao J, Wahlestedt C. Basic biology and therapeutic implications of lncRNA. Adv Drug Del Rev. 2015;87:15–24.CrossRefGoogle Scholar
  10. 10.
    Yin DD, Liu ZJ, Zhang E, Kong R, Zhang ZH, Guo RH. Decreased expression of long noncoding RNA MEG3 affects cell proliferation and predicts a poor prognosis in patients with colorectal cancer. Tumour Biol J Int Soc Oncodev Biol Med. 2015;36(6):4851–9.CrossRefGoogle Scholar
  11. 11.
    Sun L, Xue H, Jiang C, Zhou H, Gu L, Liu Y, et al. LncRNA DQ786243 contributes to proliferation and metastasis of colorectal cancer both in vitro and in vivo. Biosci Rep 2016, 36(3).Google Scholar
  12. 12.
    Huang G, Wu X, Li S, Xu X, Zhu H, Chen X. The long noncoding RNA CASC2 functions as a competing endogenous RNA by sponging miR-18a in colorectal cancer. Sci Rep. 2016;6:26524.CrossRefGoogle Scholar
  13. 13.
    Wu J, Wang D. Long noncoding RNA TCF7 promotes invasiveness and self-renewal of human non-small cell lung cancer cells. Hum cell. 2017;30(1):23–9.CrossRefGoogle Scholar
  14. 14.
    Wang Y, He L, Du Y, Zhu P, Huang G, Luo J, et al. The long noncoding RNA lncTCF7 promotes self-renewal of human liver cancer stem cells through activation of Wnt signaling. Cell Stem Cell. 2015;16(4):413–25.CrossRefGoogle Scholar
  15. 15.
    Jin FS, Wang HM, Song XY. Long non-coding RNA TCF7 predicts the progression and facilitates the growth and metastasis of colorectal cancer. Mol Med Rep. 2018;17(5):6902–8.Google Scholar
  16. 16.
    Chen J, Archer TK. Regulating SWI/SNF subunit levels via protein-protein interactions and proteasomal degradation: BAF155 and BAF170 limit expression of BAF57. Mol Cell Biol. 2005;25(20):9016–27.CrossRefGoogle Scholar
  17. 17.
    Weidle UH, Birzele F, Kollmorgen G, Ruger R. Long Non-coding RNAs and their Role in Metastasis. Cancer Genom Proteomics. 2017;14(3):143–60.CrossRefGoogle Scholar
  18. 18.
    Arce L, Yokoyama NN, Waterman ML. Diversity of LEF/TCF action in development and disease. Oncogene. 2006;25(57):7492–504.CrossRefGoogle Scholar
  19. 19.
    Cadigan KM, Waterman ML. TCF/LEFs and Wnt signaling in the nucleus. Cold Spring Harbor Perspect Biol 2012, 4(11).Google Scholar
  20. 20.
    Cui L, Guan Y, Qu Z, Zhang J, Liao B, Ma B, et al. WNT signaling determines tumorigenicity and function of ESC-derived retinal progenitors. J Clin Investig. 2016;126(10):4061.CrossRefGoogle Scholar
  21. 21.
    Hrdlickova R, Nehyba J, Bargmann W, Bose HR. Jr. Multiple tumor suppressor microRNAs regulate telomerase and TCF7, an important transcriptional regulator of the Wnt pathway. PloS One. 2014;9(2):e86990.CrossRefGoogle Scholar

Copyright information

© Japan Human Cell Society and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of GastroenterologyThe Fifth Affiliated Hospital of Sun Yat-sen UniversityZhuhaiChina
  2. 2.Department of Respiratory MedicineThe Fifth Affiliated Hospital of Sun Yat-sen UniversityZhuhaiChina
  3. 3.Department of Medical OncologyThe Fifth Affiliated Hospital of Sun Yat-sen UniversityZhuhaiChina
  4. 4.Medical record roomThe Fifth Affiliated Hospital of Sun Yat-sen UniversityZhuhaiChina

Personalised recommendations