MiR-323a-3p suppressed the glycolysis of osteosarcoma via targeting LDHA
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Accumulating evidence has demonstrated that there is critical involvement of miRNAs in the initiation and progression of cancers. Here, we showed that miR-323a-3p was significantly down-regulated in osteosarcoma (OS) tissues and cell lines. Overexpression of miR-323a-3p decreased the cell viability, colon formation and induced the apoptosis of OS cells. Using bioinformatics analysis, lactate dehydrogenase A (LDHA) was predicted as one of the down-steam targets of miR-323a-3p. Highly expressed miR-323a-3p significantly decreased both the mRNA and protein levels of LDHA. Inverse correlation between the expression of LDHA and miR-323a-3p was observed in OS tissues. Consistent with the function of LDHA in glycolysis of cancer cells, overexpression of miR-323a-3p attenuated the lactate production of OS cells. These results demonstrated that miR-323a-3p suppressed the growth of OS cells via targeting LDHA and inhibited the glycolysis of OS. This study provides insight into the molecular mechanism of miR-323a-3p in regulating OS.
KeywordsMiR-323a-3p Osteosarcoma LDHA Glycolysis
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The authors declare that they have no conflicts of interests.
- 13.Gambari R, Brognara E, Spandidos DA, Fabbri E. Targeting oncomiRNAs and mimicking tumor suppressor miRNAs: Nuew trends in the development of miRNA therapeutic strategies in oncology (Review). Int J Oncol. 2016;49(1):5–32. https://doi.org/10.3892/ijo.2016.3503.CrossRefPubMedPubMedCentralGoogle Scholar
- 18.Burns JS, Manda G. Metabolic pathways of the warburg effect in health and disease: perspectives of choice, chain or chance. Int J Mol Sci. 2017;18(12). https://doi.org/10.3390/ijms18122755.
- 31.Shahar T, Granit A, Zrihan D, Canello T, Charbit H, Einstein O, et al. Expression level of miRNAs on chromosome 14q32.31 region correlates with tumor aggressiveness and survival of glioblastoma patients. J Neuro Oncol. 2016;130(3):413–22. https://doi.org/10.1007/s11060-016-2248-0.CrossRefGoogle Scholar
- 32.Li J, Xu X, Meng S, Liang Z, Wang X, Xu M, et al. MET/SMAD3/SNAIL circuit mediated by miR-323a-3p is involved in regulating epithelial-mesenchymal transition progression in bladder cancer. Cell Death Dis. 2017;8(8):e3010. https://doi.org/10.1038/cddis.2017.331.CrossRefPubMedPubMedCentralGoogle Scholar